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UniProtKB/Swiss-Prot entry Q7XDC8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MDHC_ORYSJ
Primary accession number Q7XDC8
Secondary accession numbers Q0IWZ1 Q109J3 Q9FWH5
Integrated into Swiss-Prot on December 20, 2005
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 42)
Name and origin of the protein
Protein name Malate dehydrogenase, cytoplasmic
Synonyms EC 1.1.1.37
PP37
Gene name
OrderedLocusNames: Os10g0478200, LOC_Os10g33800
ORFNames: OSJNBa0055P24.3
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
DOI=10.1080/10425170410001710565; PubMed=15620222 [NCBI, ExPASy, EBI, Israel, Japan]
Lin C.F., Jiang R.H., Jiang L.Z., Qian X.Y., Attia K., Yang J.S.;
"Cloning, characterization and prokaryotic expression of cytosolic malate dehydrogenase from Oryza sativa.";
DNA Seq. 15:314-318(2004).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1126/science.1083523; PubMed=12791992 [NCBI, ExPASy, EBI, Israel, Japan]
Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
"In-depth view of structure, activity, and evolution of rice chromosome 10.";
Science 300:1566-1569(2003).
[3]
PROTEIN SEQUENCE OF 2-11; 41-50; 144-153 AND 151-160, AND TISSUE SPECIFICITY.
STRAIN=cv. Nipponbare;
TISSUE=Panicle, Sheath, and Stem;
DOI=10.1093/nar/gkh020; PubMed=14681440 [NCBI, ExPASy, EBI, Israel, Japan]
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003.";
Nucleic Acids Res. 32:D388-D392(2004).
[4]
IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, AND PHOSPHORYLATION.
STRAIN=cv. Nipponbare;
DOI=10.1007/s11103-005-4013-1; PubMed=16028114 [NCBI, ExPASy, EBI, Israel, Japan]
Khan M.M.K., Jan A., Karibe H., Komatsu S.;
"Identification of phosphoproteins regulated by gibberellin in rice leaf sheath.";
Plant Mol. Biol. 58:27-40(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF353203; AAK26431.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC037425; AAG13573.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP008216; BAF26774.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DP000086; AAP54283.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DP000086; ABG66141.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001064860.1; -.
UniGene Os.21850
3D structure databases
HSSP P11708; 4MDH. [HSSP ENTRY / PDB]
ModBase Q7XDC8.
Organism-specific databases
Gramene Q7XDC8; -.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR011274; Malate_DHase_NAD-dep_euk.
IPR010945; Malate_DHase_SF1.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR23382; MDH_SF1; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
ProDom PD003052; Mal_dehydrog; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01759; MalateDH-SF1; 1.
TIGR01758; MDH_euk_cyt; 1.
PROSITE PS00068; MDH; 1.
BLOCKS Q7XDC8.
Genome annotation databases
GeneID 4348905; -.
KEGG osa:4348905; -.
NMPDR fig|39947.1.peg.13505; -.
Other
ProtoNet Q7XDC8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   332  331     Malate dehydrogenase, cytoplasmic. PRO_0000113418
NP_BIND   12    18  7     NAD (By similarity). 
NP_BIND   130   132  3     NAD (By similarity). 
ACT_SITE   188   188        Proton acceptor (By similarity). 
BINDING   93    93        Substrate carboxyl (By similarity). 
BINDING   99    99        Substrate carboxyl (By similarity). 
BINDING   106   106        NAD (By similarity). 
BINDING   113   113        NAD (By similarity). 
BINDING   132   132        Substrate (By similarity). 
BINDING   163   163        Substrate carboxyl (By similarity). 
Sequence information
Length: 332 AA [This is the length of the unprocessed precursor] Molecular weight: 35569 Da [This is the MW of the unprocessed precursor] CRC64: EB1E3187DCC4DF42 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPATES LNGLKMELVD 

        70         80         90        100        110        120 
AAFPLLKGIV ATTDVVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEAH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQISE KLNVQVTDVK 

       190        200        210        220        230        240 
NAIIWGNHSS TQYPDVNHAT VKTPSGEKPV RELVADDEWL NTEFISTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
QSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PAGLIYSFPV TCSGGEWTIV 

       310        320        330 
QGLPIDEFSR KKMDATAQEL SEEKTLAYSC LN 

Q7XDC8 in FASTA format

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