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[1]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1038/nature01554; PubMed=12712197 [NCBI, ExPASy, EBI, Israel, Japan]
Galagan J.E.,
Calvo S.E.,
Borkovich K.A.,
Selker E.U.,
Read N.D.,
Jaffe D.B.,
FitzHugh W.,
Ma L.-J.,
Smirnov S.,
Purcell S.,
Rehman B.,
Elkins T.,
Engels R.,
Wang S.,
Nielsen C.B.,
Butler J.,
Endrizzi M.,
Qui D.,
Ianakiev P., ![]()
Bell-Pedersen D.,
Nelson M.A.,
Werner-Washburne M.,
Selitrennikoff C.P.,
Kinsey J.A.,
Braun E.L.,
Zelter A.,
Schulte U.,
Kothe G.O.,
Jedd G.,
Mewes H.-W.,
Staben C.,
Marcotte E.,
Greenberg D.,
Roy A.,
Foley K.,
Naylor J.,
Stange-Thomann N.,
Barrett R.,
Gnerre S.,
Kamal M.,
Kamvysselis M.,
Mauceli E.W.,
Bielke C.,
Rudd S.,
Frishman D.,
Krystofova S.,
Rasmussen C.,
Metzenberg R.L.,
Perkins D.D.,
Kroken S.,
Cogoni C.,
Macino G.,
Catcheside D.E.A.,
Li W.,
Pratt R.J.,
Osmani S.A.,
DeSouza C.P.C.,
Glass N.L.,
Orbach M.J.,
Berglund J.A.,
Voelker R.,
Yarden O.,
Plamann M.,
Seiler S.,
Dunlap J.C.,
Radford A.,
Aramayo R.,
Natvig D.O.,
Alex L.A.,
Mannhaupt G.,
Ebbole D.J.,
Freitag M.,
Paulsen I.,
Sachs M.S.,
Lander E.S.,
Nusbaum C.,
Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
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- FUNCTION: Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).
- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine.
- ENZYME REGULATION: Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 (By similarity).
- SUBCELLULAR LOCATION: Nucleus (By similarity).
- MISCELLANEOUS: In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
- SIMILARITY: Belongs to the DOT1 family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 531 AA [This is the length of the unprocessed precursor] |
Molecular weight: 59294 Da [This is the MW of the unprocessed precursor] |
CRC64: D1ED4AFD7EBDD1BB [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSIFNQKSKF KIKTEIRTVR ETVERPKLKA STSSSSIPTT GSSQHSNVRI AGAGSSLSRA
70 80 90 100 110 120
SSTPRLNGTS HTTSTSSSAR ASLTKVTTIP TARRIQSASP IPSINGDGRS SRKRRAIKAS
130 140 150 160 170 180
SRSPISPHFS EDSDSDSNGD ENWEDNLDAR KRRKRVHDER PVDVNRKLRH PKIWKGEEAD
190 200 210 220 230 240
QVERIPGPAI IHAAKVASLE HKCKPALGLK NEEVAVRLQY PGARYPERYE LVEGKDKIDA
250 260 270 280 290 300
VKDIEKVVQL AASTYLTDEE GKIFLDHQTG ILRKLVKSKN RSDGPGFKAA LREYNDEFLK
310 320 330 340 350 360
LMRKGVIVRN LDKMHGIPRE LVDFILDQVY DRTVAPRVEL LAKYQNGSDN VYGELNHPFI
370 380 390 400 410 420
SDILERTNLT SDKVFVDLGS GVGNVTLQAA LEIGCESWGC EMMENACNLA EAQYDEFLAR
430 440 450 460 470 480
CRLWGVKPGK VRLERGDFRK NEKILEVLKR ADVVLVNNQA FTSQLNDNLV SMFLDLKIGC
490 500 510 520 530
KIISLKTFVH DNKNAENDVA TSILDVEHLT YPEGYVSWTG ASGTYCISTR R
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Q7SB74 in FASTA format |
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