ID   DNLI4_NEUCR             Reviewed;        1046 AA.
AC   Q7SB49;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   04-NOV-2008, entry version 39.
DE   RecName: Full=DNA ligase 4;
DE            EC=6.5.1.1;
DE   AltName: Full=DNA ligase IV;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] 4;
DE   AltName: Full=DNA repair and recombination protein mus-53;
GN   Name=lig-4; Synonyms=mus-53; ORFNames=NCU06264;
OS   Neurospora crassa.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
OC   Neurospora.
OX   NCBI_TaxID=5141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=17003123; DOI=10.1073/pnas.0604477103;
RA   Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H.;
RT   "Nonhomologous chromosomal integration of foreign DNA is completely
RT   dependent on MUS-53 (human Lig4 homolog) in Neurospora.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   MEDLINE=22598136; PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Involved in ds DNA break repair. Has a role in non-
CC       homologous integration (NHI) pathways where it is required in the
CC       final step of non-homologus end-joining.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + diphosphate +
CC       (deoxyribonucleotide)(n+m).
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC   -!- SIMILARITY: Contains 2 BRCT domains.
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DR   EMBL; AB261106; BAF34364.1; -; Genomic_DNA.
DR   EMBL; AABX02000025; EAA33632.1; -; Genomic_DNA.
DR   RefSeq; XP_962868.1; -.
DR   GeneID; 3879007; -.
DR   KEGG; ncr:NCU06264; -.
DR   NMPDR; fig|5141.1.peg.4052; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR   InterPro; IPR001357; BRCT.
DR   InterPro; IPR000977; DNA_ligase.
DR   InterPro; IPR012309; DNA_ligase_A_C.
DR   InterPro; IPR012310; DNA_ligase_A_M.
DR   InterPro; IPR016059; DNA_ligase_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF00533; BRCT; 3.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   SMART; SM00292; BRCT; 2.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS50172; BRCT; 2.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA recombination;
KW   DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus;
KW   Repeat.
FT   CHAIN         1   1046       DNA ligase 4.
FT                                /FTId=PRO_0000278384.
FT   DOMAIN      738    836       BRCT 1.
FT   DOMAIN      932   1045       BRCT 2.
FT   ACT_SITE    327    327       N6-AMP-lysine intermediate (By
FT                                similarity).
SQ   SEQUENCE   1046 AA;  118910 MW;  072B0B5B806A3146 CRC64;
     MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEQPRNHS KTFPFSDLFR TLFNPLIDCK
     PSTSGGTVRG PKPGRGGHFS KVSYHEQRRH IIERFMSRWR SEVGNDFYPA MRLILPDKDR
     DRGVYGLKEN TIGKLLVKVM KIDRNSEDGY NLMHWKLPGG QSGVSRSVGD FAGRCLEVVS
     KRAMRAQPGD LTIADVNVLL DRLAAASGEA EQLPIFEEFY RQMNAEEMMW LVRIILKDMR
     VGATERTFLN LWHPDAEALF SVSSSLRRVC WELFDPEFRL EQQETGIKLM QCFQPQLAQF
     QMTTTWEKLV KNLGVTEENP EFWIEEKLDG ERMQMHMIED DTVPGGFRFA FWSRKAKDYT
     YLYGESLGDE QSALTRHLHK AFDDGVRNLI LDGEMITWDI DIDKMVPFGT LKTAALEQQK
     NPSKAGPRPL YRVFDILLLN DKPLTEYTLN DRRRALERAV VGVHRRLEIL PFERATSPDA
     IEPLLRRVVA EASEGLVLKN PRSRYSLNSR NNDWIKVKPE YMSDFGESLD CVVVGGYFGS
     GRRGGTLSSF LCGVRVSQNF IKSGNASAEK CLSFVKVGGG FKAEDYAEIR HHTEGKWQDW
     DPSSPPTEYI ELGGGEKLQY EKPDVWIRPS DSVVISVKAA SITQSDQFAM GWTLRFPRFR
     KLRLDRAWDS ALDMDEFEVL RSKIKDQEQE RKKMEMENRK RKPATKRARK DLVIAGMSDP
     SSSSAATPVI APKETREASK RLFEGLDFCV LSDSLKPNKM TKPALEKLIK DHGGRIHQQV
     MDHSGQGKII IPIADKNVIK VASLRKANPE MDIIRPKWIF DCLVQPMPFT KQKENKKGYL
     LPFEPTHLFH SGSEETSEEA EQAVDKFGDS YAGDLADINE LKAIMEGMES DDYVSDSDWD
     SDSGRGRGGG DGFDMNHFLD HLEEQGTSLD DLRSFMFRRC RVFFALPSAG NGDGAAESKA
     LRLKNYIRFG NGKVVDELET ATHVVVVTAP LGESSKKEER EIAAELRYKI SLREMGSPMP
     RIVKGEWVED SWKEGTVVDE EEYVAG
//