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UniProtKB/Swiss-Prot entry Q7RTX0


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TS1R3_HUMAN
Primary accession number Q7RTX0
Secondary accession numbers Q5TA49 Q8NGW9
Integrated into Swiss-Prot on January 4, 2005
Sequence was last modified on March 29, 2005 (Sequence version 2)
Annotations were last modified on    June 10, 2008 (Entry version 51)
Name and origin of the protein
Protein name Taste receptor type 1 member 3 [Precursor]
Synonym Sweet taste receptor T1R3
Gene name
Name: TAS1R3
Synonyms: T1R3, TR3
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE.
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes.";
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[3]
IDENTIFICATION, AND FUNCTION.
DOI=10.1073/pnas.072090199; PubMed=11917125 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E.;
"Human receptors for sweet and umami taste.";
Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002).
[4]
FUNCTION.
DOI=10.1290/1543-706X(2003)039<0080:TRTIAE>2.0.CO;2; PubMed=12892531 [NCBI, ExPASy, EBI, Israel, Japan]
Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M.;
"Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose.";
In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003).
[5]
REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, AND MUTAGENESIS OF ALA-537 AND PHE-540.
DOI=10.1074/jbc.M406779200; PubMed=15299024 [NCBI, ExPASy, EBI, Israel, Japan]
Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M.;
"The cysteine-rich region of T1R3 determines responses to intensely sweet proteins.";
J. Biol. Chem. 279:45068-45075(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB065647; BAC05873.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL139287; CAI23184.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BK000152; DAA00013.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_689414.1; -.
UniGene Hs.74375
3D structure databases
ModBase Q7RTX0.
Protein family/group databases
GPCRDB Q7RTX0; TS1R3_HUMAN.
Organism-specific databases
HGNC HGNC:15661; TAS1R3.
GeneLynx TAS1R3; Homo sapiens.
GenAtlas TAS1R3.
MIM 605865; gene. [NCBI / EBI]
PharmGKB PA38012; -.
GeneCards Q7RTX0.
Gene expression databases
ArrayExpress Q7RTX0; -.
CleanEx HS_TAS1R3; -.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred by curator from UniProtKB).
GO:0046982; Molecular function: protein heterodimerization activity (non-traceable author statement from UniProtKB).
GO:0008527; Molecular function: taste receptor activity (inferred from direct assay from UniProtKB).
GO:0001582; Biological process: detection of chemical stimulus involved in sensory perception of sweet taste (inferred from direct assay from HGNC).
GO:0007186; Biological process: G-protein coupled receptor protein signaling pathway (inferred by curator from HGNC).
GO:0050917; Biological process: sensory perception of umami taste (inferred from direct assay from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR001828; ANF_lig_bd_rcpt.
IPR000337; GPCR_3.
IPR000068; GPCR_3_Ca_sens_rcpt-rel.
IPR011500; GPCR_3_NCR.
Graphical view of domain structure.
Pfam PF00003; 7tm_3; 1.
PF01094; ANF_receptor; 1.
PF07562; NCD3G; 1.
Pfam graphical view of domain structure.
PRINTS PR00592; CASENSINGR.
PR00248; GPCRMGR.
PROSITE PS00979; G_PROTEIN_RECEP_F3_1; FALSE_NEG.
PS00980; G_PROTEIN_RECEP_F3_2; 1.
PS00981; G_PROTEIN_RECEP_F3_3; FALSE_NEG.
PS50259; G_PROTEIN_RECEP_F3_4; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q7RTX0.
Genome annotation databases
Ensembl ENSG00000169962; Homo sapiens. [Contig view]
GeneID 83756; -.
KEGG hsa:83756; -.
Phylogenomic databases
HOGENOM Q7RTX0; -.
HOVERGEN Q7RTX0; -.
Other
DrugBank DB00168; Aspartame.
SOURCE TAS1R3; Homo sapiens.
GPCRDB-Snakes Q7RTX0.
ProtoNet Q7RTX0.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
G-protein coupled receptor; Glycoprotein; Membrane; Polymorphism; Receptor; Sensory transduction; Signal; Taste; Transducer; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    20  20     Potential. 
CHAIN   21   852  832     Taste receptor type 1 member 3. PRO_0000012961
TOPO_DOM   21   570  550     Extracellular (Potential). 
TRANSMEM   571   591  21     1 (Potential). 
TOPO_DOM   592   603  12     Cytoplasmic (Potential). 
TRANSMEM   604   624  21     2 (Potential). 
TOPO_DOM   625   639  15     Extracellular (Potential). 
TRANSMEM   640   660  21     3 (Potential). 
TOPO_DOM   661   682  22     Cytoplasmic (Potential). 
TRANSMEM   683   703  21     4 (Potential). 
TOPO_DOM   704   729  26     Extracellular (Potential). 
TRANSMEM   730   750  21     5 (Potential). 
TOPO_DOM   751   762  12     Cytoplasmic (Potential). 
TRANSMEM   763   783  21     6 (Potential). 
TOPO_DOM   784   789  6     Extracellular (Potential). 
TRANSMEM   790   810  21     7 (Potential). 
TOPO_DOM   811   852  42     Cytoplasmic (Potential). 
REGION   536   545  10     Required for brazzein responsiveness. 
CARBOHYD   85    85        N-linked (GlcNAc...) (Potential). 
CARBOHYD   130   130        N-linked (GlcNAc...) (Potential). 
CARBOHYD   264   264        N-linked (GlcNAc...) (Potential). 
CARBOHYD   285   285        N-linked (GlcNAc...) (Potential). 
CARBOHYD   380   380        N-linked (GlcNAc...) (Potential). 
CARBOHYD   411   411        N-linked (GlcNAc...) (Potential). 
CARBOHYD   432   432        N-linked (GlcNAc...) (Potential). 
CARBOHYD   475   475        N-linked (GlcNAc...) (Potential). 
VARIANT   757   757  1     C -> R (in dbSNP:rs307377 [NCBI]). VAR_020788 
MUTAGEN   537   537        A->G: Retains partial activity toward brazzein; however response to other sweeteners tested is suppressed. 
MUTAGEN   537   537        A->P: Receptor unresponsive to all sweeteners tested. 
MUTAGEN   537   537        A->T,S,E,V: Abolished the response to brazzein. 
MUTAGEN   540   540        F->A,H: Reduces the response to brazzein and monellin. 
MUTAGEN   540   540        F->L: Reduces the response to monellin. 
MUTAGEN   540   540        F->Y,P: Reduces the response to brazzein; P-540 also enhances responses to the small molecule sweeteners. 
CONFLICT   852   852        E -> EAPILHPARHHSSGRPGGGTQAYAQGRARRGRGGTQLPTL PFAQWVDSIMTVTSTRDRAQDLTPARRQAHTGSPVRVPGS APSRA (in Ref. 1; BAC05873). 
Sequence information
Length: 852 AA [This is the length of the unprocessed precursor] Molecular weight: 93386 Da [This is the MW of the unprocessed precursor] CRC64: 9690DEC23E75EB3E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP 

        70         80         90        100        110        120 
VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA 

       130        140        150        160        170        180 
GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF 

       190        200        210        220        230        240 
PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE 

       250        260        270        280        290        300 
GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS 

       310        320        330        340        350        360 
EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG 

       370        380        390        400        410        420 
LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP 

       430        440        450        460        470        480 
VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT 

       490        500        510        520        530        540 
ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF 

       550        560        570        580        590        600 
CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ 

       610        620        630        640        650        660 
ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV 

       670        680        690        700        710        720 
ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV 

       730        740        750        760        770        780 
HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGCYNR ARGLTFAMLA YFITWVSFVP 

       790        800        810        820        830        840 
LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ 

       850 
NDGNTGNQGK HE 

Q7RTX0 in FASTA format

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