NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=PCC 7421;
DOI=10.1093/dnares/10.4.137; PubMed=14621292 [NCBI, ExPASy, EBI, Israel, Japan]
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.;
"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids.";
DNA Res. 10:137-145(2003).

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000045; BAC91566.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_926571.1; -.

3D structure databases

ModBase

Q7NFA1.

Enzyme and pathway databases

BioCyc

GVIO251221:GLL3625-MON; -.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGR02029; AcsF; 1.

Genome annotation databases

GeneID

2602029; -.

GenomeReviews

BA000045_GR; gll3625.

KEGG

gvi:gll3625; -.

NMPDR

fig|251221.1.peg.3625; -.

Phylogenomic databases

HOGENOM

Q7NFA1; -.

Genome annotation databases

CMR

Q7NFA1; gll3625.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 346`	`346`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_0000217529`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 40171 Da [This is the MW of the unprocessed precursor]

CRC64: 60FA7605D5EB8749 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVDALREANT ASQSERRETL LTPRFYTTDF EALAKLDISA MEADFEALLA EFQADYNREH 

        70         80         90        100        110        120 
FQRDAGFVEQ AHQIDPQWRA LMLEFLERSC TSEFSGFLLY KEISRRLKAA NPLLAECFKA 

       130        140        150        160        170        180 
MSRDEARHAG FLNKAMGDYD LGLDLSNLAK QKKYTFFKPK FIFYATYLSE KIGYWRYIAI 

       190        200        210        220        230        240 
FRHLERHPEN RIHPLFNYFK NWCQDENRHG DFFGLILKSQ PQLLKGPINR LWVRFFLLSF 

       250        260        270        280        290        300 
CLTMYLNDRK PHNLYSALGL DASHYAMEVL RQTNLDSGKV FPLILDLDNP RFQRHLDRCA 

       310        320        330        340 
DLNLQLEALS GKSPLARLPV IAALAGTIAQ LFLLKPVAPP APEMVR

Q7NFA1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools