RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Hirame rhabdovirus (strain Korea/CA 9703/1997) (HIRRV)

[TaxID: 453457]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Novirhabdovirus.

Virus hosts

Acanthopagrus schlegeli (Black porgy) [TaxID: 72011]
Paralichthys olivaceus (Japanese flounder) [TaxID: 8255]
Plecoglossus altivelis (Ayu) [TaxID: 61084]
Sebastes inermis [TaxID: 160818]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/j.virusres.2004.06.004; PubMed=15567027 [NCBI, ExPASy, EBI, Israel, Japan]
Kim D.H., Oh H.K., Eou J.I., Seo H.J., Kim S.K., Oh M.J., Nam S.W., Choi T.J.;
"Complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish.";
Virus Res. 107:1-9(2005).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF104985; AAQ73462.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_919035.1; -.

3D structure databases

ModBase

Q77SJ8.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q77SJ8.

Genome annotation databases

GeneID

2559539; -.

Other

ProtoNet

Q77SJ8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1986`	`1986`	`Large structural protein.`	`PRO_0000297836`
`DOMAIN`	`560 757`	`198`	`RdRp catalytic.`

Sequence information

Length: 1986 AA [This is the length of the unprocessed precursor]

Molecular weight: 224461 Da [This is the MW of the unprocessed precursor]

CRC64: 3A6254B6704D0C59 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDFFDLDIEI KQERLPAECS LNSPLNISLS SQLTDRMTPQ NENIRRQRER IRTHTKTHSR 

        70         80         90        100        110        120 
IKHLSKLDND STRLHARLTE DLIKLQHLEV DSPVFDNWAL LTSYYAALDY TLPERASFDW 

       130        140        150        160        170        180 
GQAAPYWNLY TQLRTILLQS QKIRKKDRGV REIYSCGPLR LEFVEGTVLY FTDKQSGGEF 

       190        200        210        220        230        240 
TKSGELPSIT PYADFLAWVK IISQRAQAVL MAVILRVTDK GLSPLPESLL SVYQTVDDIL 

       250        260        270        280        290        300 
KRAGQPAIDL LKLWEPLVIT KLGELLGDRF GLEEDFRLTI RGEATRLAKK LAITNGLNRL 

       310        320        330        340        350        360 
MTVLDSQTEA QPLFQFFGLF KHFAYPRVFS RDTIQAIQEV SDRPSSISAA EFLHDQCEIR 

       370        380        390        400        410        420 
KEFYIRYLKA YHRAPGLDLS ALSPSSFLRE SLEHGKIPNE KSPHYSNKEW YFIKFTKSIE 

       430        440        450        460        470        480 
WPISDTLSTF LSDKAITRDR AAWIEEGHSG RDMSEKRLLL KFIKENFSSV AEIVAAADAI 

       490        500        510        520        530        540 
YNNEGDRLIA LKVKEMELKI KGRGFGLMTF MPRLLQVLRE SIAKKTSKLF PEITMTSSDL 

       550        560        570        580        590        600 
DMKKRKFMLS KRSDDRRGFI HINKSLDINK FCTSQRQFNS SAVFSSLDEM MGTFPLFSRV 

       610        620        630        640        650        660 
HEIFEKTWIV DGSASDPPDL SHFTRILEEC RLHGIEAPHV WADGVFSGLK GGIEGLCQYV 

       670        680        690        700        710        720 
WTICLLLRVE RVMQKTKLTH YILAQGDNVI ITIIVPVEIH RDGIISDQES RRLLTLSRNI 

       730        740        750        760        770        780 
DLSLESELEK SGLTLKIEET LTSENISIYG KDLHCPQHLT LALKKAASAA IISSEQYQDI 

       790        800        810        820        830        840 
PTFLSGLGTS LESLSECVNS KTGAHLFGVL MGVAGWKDLA THQTWRGWRY PYHKAPLSGR 

       850        860        870        880        890        900 
VRASDMKIGK GEAVELTIPV MSPRQQGKET LRELLANSLL GSALGMLAFP TPIDLEKRGV 

       910        920        930        940        950        960 
GDYITHRLAI ARKALLSEKL DPHIEKRVRS ACNLPLSSRV DLSKLFDSPF SLNLATEEDA 

       970        980        990       1000       1010       1020 
TAVIKRQAKK TLRLQEIGND KLRAQIGNMD KGIAALDADL AGAETINPRL NHMIRDITDE 

      1030       1040       1050       1060       1070       1080 
KESEMFVTKF ASARTMRTLA MGDSSEVPIV VLLEKKSQQK ELYTIWRARR PHATMWKCST 

      1090       1100       1110       1120       1130       1140 
VLAKGLRDIS WGKTIVGVTS PSPIEAMETT HIDPTDWEDS RSRETLSINY YLSRAGIDEQ 

      1150       1160       1170       1180       1190       1200 
TAKLTRGSLV PYYGTQTKPL IAKAYLELKG NPRTNKALLL LSVRESLVKT GSNLDELIMQ 

      1210       1220       1230       1240       1250       1260 
LCSHALDIDA ASLPALRAQE EATAGEGLRG GIKESMSPVG PDNFYTNITH KVFNRKWVTP 

      1270       1280       1290       1300       1310       1320 
YHVNIADFII QGLIETRKHL ILNEKMDGLL PLSSVKCTAC FRKKEREFFD IPEGPTWKND 

      1330       1340       1350       1360       1370       1380 
STTSDPAYTY FTTWCDLPRV STLPTMDQQS ATRLLGRGLS LNRPTAGEII TKFYSMSMES 

      1390       1400       1410       1420       1430       1440 
QRLLHPVDLL LGYGEGVVFG YIRSQHIHHG ALFQTKRETL TNKLRKFILD TKTQHAKQIG 

      1450       1460       1470       1480       1490       1500 
YLFQDEDSLH ELMAQGLCPY VPRSIPLTIT ELTNACAITT IRATEVILSA GSRVALMPVQ 

      1510       1520       1530       1540       1550       1560 
AIDETDVDNS RLAANTMQTI LGDSRPMNPV YLDCDLTTNM TAWESSIELD VLKSENFHID 

      1570       1580       1590       1600       1610       1620 
GLLMDLTARE LPISDTPWKQ RDWTCSNDPR IIAKGIKTKS LFIHQGVAEA LNMTPDLLVV 

      1630       1640       1650       1660       1670       1680 
IGGGLGGCAV PYLQEWADVP LIFATLFDER ERISEDGDLV VPPEILVRGM APRMIERELL 

      1690       1700       1710       1720       1730       1740 
EAELCDVTND GNRRLLTRLV KKNRGKGTVV LIDEIENRGA PESLLQSSLQ DLVRRLDKVC 

      1750       1760       1770       1780       1790       1800 
TLTSIHTVRE STVEQFAQRT NSIKRDRKTV TLHWNRYNRR DQFEALVIVK GEETRSDYHV 

      1810       1820       1830       1840       1850       1860 
STATAAQAFR KIDEQLEVEG RLSATRWSLP TLPAREKEIL FGYVSSVFLK TNLVLSADDM 

      1870       1880       1890       1900       1910       1920 
DRETLLETIE DTAPGLISWK EKLEHRDHAF RSDIDEKGIT QDKVFNLICL AWVITGLRYG 

      1930       1940       1950       1960       1970       1980 
IWETDAQSII TKTVYITRGP KLCPLGEKPK RVFASFKLQS DKRVEDAKGF LSALLHLEGF 


FPLGRQ

Q77SJ8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools