NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 10895 / NRRL Y-1056 / CBS 109.51;
DOI=10.1126/science.1095781; PubMed=15001715 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.;
"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome.";
Science 304:304-307(2004).

Comments

FUNCTION: First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end (By similarity).
CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H₂O = a polynucleotide + phosphate.
COFACTOR: Divalent ions (By similarity).
SUBUNIT: The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the fungal TPase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016819; AAS53240.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_985416.1; -.

3D structure databases

ModBase

Q755F7.

Organism-specific databases

AGD

AFL134W; -.

Family and domain databases

InterPro

IPR004206; mRNA_triPase.
Graphical view of domain structure.

Pfam

PF02940; mRNA_triPase; 1.
Pfam graphical view of domain structure.

BLOCKS

Q755F7.

Genome annotation databases

4621643; -.

fig|33169.1.peg.3061; -.

Phylogenomic databases

HOGENOM

Q755F7; -.

Other

ProtoNet

Q755F7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Hydrolase; mRNA capping; mRNA processing; Nucleus.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 478`	`478`		`mRNA-capping enzyme subunit beta.`	`PRO_0000210111`

Sequence information

Length: 478 AA [This is the length of the unprocessed precursor]

Molecular weight: 53999 Da [This is the MW of the unprocessed precursor]

CRC64: 1D734613FA2401FC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSEKQEGSP RRVLSLSDLV NREDSKDASG NTAGAALKPL SNDEVRRRLA HEDYSSNTVS 

        70         80         90        100        110        120 
QVDDETDTDD GLGEVAFDRA DFRFDFEGHQ RSGGRASGEA EAESGGPGHE KATQQPSDAI 

       130        140        150        160        170        180 
ATSPDRESTE PRAMDIFEER ASLESKKNNL RKDLRVLNEI ASTARPGRYK VAPIWAQKWK 

       190        200        210        220        230        240 
PTVRALQNVN SKDLMKIDVS FTQVIPDDDL TKSVQDWVYA TLLSIPPEQR QYVEVEMKFG 

       250        260        270        280        290        300 
ILMDRSSDSQ RVTPPVSSQT VYMEADARMK PDVDERVFVE LNRYVKGISE LTENTGKFNI 

       310        320        330        340        350        360 
IESHNKDEMY RAGINTQRPR FLRMSKDVKT GRVGEFIEKR RISQLLLFSP KDSYDVKISI 

       370        380        390        400        410        420 
NVELPVPEND PPEKYMGQAP LNSRTKERIS YIHNDSCTRI DITKVTNHNK GKRDDAEVTH 

       430        440        450        460        470 
EIELELNSQA LLAAFDKIAQ DSKDYATIVR TFLNNGTIIR RKLTSLSYEI FEGGKKVV

Q755F7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools