ID   EBF2_ARATH              Reviewed;         623 AA.
AC   Q708Y0;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   22-JUL-2008, entry version 43.
DE   RecName: Full=EIN3-binding F-box protein 2;
GN   Name=EBF2; OrderedLocusNames=At5g25350; ORFNames=F18G18.90;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION, TISSUE
RP   SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION WITH CUL1;
RP   SKP1A/ASK1; SKP1B/ASK2; EIN3 AND EIL1.
RX   PubMed=14675533; DOI=10.1016/S0092-8674(03)00968-1;
RA   Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S.,
RA   Koncz C., Genschik P.;
RT   "EIN3-dependent regulation of plant ethylene hormone signaling by two
RT   Arabidopsis F box proteins: EBF1 and EBF2.";
RL   Cell 115:679-689(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016721; PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of SCF(EBF1) E3 ubiquitin ligase complexes,
CC       which may mediate the ubiquitination and subsequent proteasomal
CC       degradation of target proteins (probably including EIN3 and EIL1).
CC       Regulator of the ethylene signaling cascade by modulating the
CC       stability of EIN3 and EIL1 proteins.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC       Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, EIN3, and EIL1.
CC   -!- INTERACTION:
CC       P43291:ASK1; NbExp=1; IntAct=EBI-593623, EBI-401164;
CC       Q94AH6:CUL1; NbExp=1; IntAct=EBI-593623, EBI-532411;
CC       O24606:EIN3; NbExp=1; IntAct=EBI-593623, EBI-593576;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Ubiquitous.
CC   -!- INDUCTION: EIN3-dependent induction by ethylene.
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- SIMILARITY: Contains 16 LRR (leucine-rich) repeats.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AJ609239; CAE75865.1; -; Genomic_RNA.
DR   EMBL; AY485830; AAR27072.1; -; mRNA.
DR   EMBL; AC006258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK227858; BAE99835.1; -; mRNA.
DR   RefSeq; NP_197917.1; -.
DR   UniGene; At.19865; -.
DR   IntAct; Q708Y0; -.
DR   GeneID; 832607; -.
DR   GenomeReviews; BA000015_GR; AT5G25350.
DR   KEGG; ath:AT5G25350; -.
DR   NMPDR; fig|3702.1.peg.24733; -.
DR   TAIR; At5g25350; -.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR001810; F-box.
DR   InterPro; IPR001611; LRR.
DR   InterPro; IPR006553; LRR_cys_sub-typ.
DR   Pfam; PF00646; F-box; 1.
DR   Pfam; PF00560; LRR_1; 3.
DR   SMART; SM00256; FBOX; 1.
DR   SMART; SM00367; LRR_CC; 1.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Ethylene signaling pathway; Leucine-rich repeat;
KW   Nucleus; Repeat; Ubl conjugation pathway.
FT   CHAIN         1    623       EIN3-binding F-box protein 2.
FT                                /FTId=PRO_0000272246.
FT   DOMAIN       52    106       F-box.
FT   REPEAT       69     86       LRR 1.
FT   REPEAT       87    138       LRR 2.
FT   REPEAT      139    166       LRR 3.
FT   REPEAT      167    193       LRR 4.
FT   REPEAT      194    236       LRR 5.
FT   REPEAT      237    263       LRR 6.
FT   REPEAT      264    287       LRR 7.
FT   REPEAT      288    312       LRR 8.
FT   REPEAT      313    341       LRR 9.
FT   REPEAT      342    367       LRR 10.
FT   REPEAT      368    392       LRR 11.
FT   REPEAT      393    448       LRR 12.
FT   REPEAT      449    475       LRR 13.
FT   REPEAT      476    503       LRR 14.
FT   REPEAT      504    525       LRR 15.
FT   REPEAT      526    549       LRR 16.
SQ   SEQUENCE   623 AA;  66061 MW;  990E2D9B098A11B6 CRC64;
     MSGIFRFSGD EDCLLGGSMY LSPGSCPGVY YPARKRLRVA ATSFYSGFEE KQTSIDVLPE
     ECLFEILRRL PSGQERSACA CVSKHWLNLL SSISRSEVNE SSVQDVEEGE GFLSRSLEGK
     KATDLRLAAI AVGTSSRGGL GKLQIRGSGF ESKVTDVGLG AVAHGCPSLR IVSLWNLPAV
     SDLGLSEIAR SCPMIEKLDL SRCPGITDSG LVAIAENCVN LSDLTIDSCS GVGNEGLRAI
     ARRCVNLRSI SIRSCPRIGD QGVAFLLAQA GSYLTKVKLQ MLNVSGLSLA VIGHYGAAVT
     DLVLHGLQGV NEKGFWVMGN AKGLKKLKSL SVMSCRGMTD VGLEAVGNGC PDLKHVSLNK
     CLLVSGKGLV ALAKSALSLE SLKLEECHRI NQFGLMGFLM NCGSKLKAFS LANCLGISDF
     NSESSLPSPS CSSLRSLSIR CCPGFGDASL AFLGKFCHQL QDVELCGLNG VTDAGVRELL
     QSNNVGLVKV NLSECINVSD NTVSAISVCH GRTLESLNLD GCKNITNASL VAVAKNCYSV
     NDLDISNTLV SDHGIKALAS SPNHLNLQVL SIGGCSSITD KSKACIQKLG RTLLGLNIQR
     CGRISSSTVD TLLENLWRCD ILY
//