RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

[TaxID: 128951]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae; Ebola-like viruses.

Virus hosts

Epomops franqueti (Franquet's epauleted bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Chain P.S.G., Ichou M.A., Malfatti S.A., Hajjaj A., Vergez L.M., Paragas J., Do L.H., Jahrling P.B., Smith K.L., McCready P.M., Ibrahim M.S.; Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBCELLULAR LOCATION: Cytoplasm. Virion (By similarity).
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY354458; AAQ55053.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q6V1Q2.

Family and domain databases

InterPro

IPR017235; RNA-dir_RNA_pol_filovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037548; RNA_pol_Filoviridae; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2212`	`2212`	`Large structural protein.`	`PRO_0000245050`
`DOMAIN`	`625 809`	`185`	`RdRp catalytic.`

Sequence information

Length: 2212 AA [This is the length of the unprocessed precursor]

Molecular weight: 252791 Da [This is the MW of the unprocessed precursor]

CRC64: 56B8C5F52ED3C572 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRNCKLPKHI YRLKYDVTVT 

        70         80         90        100        110        120 
KFLSDVPVAT LPIDFIVPIL LKALSGNGFC PVEPRCQQFL DEIIKYTMQD ALFLKYYLKN 

       130        140        150        160        170        180 
VGAQEDCVDD HFQEKILSSI QGNEFLHQMF FWYDLAILTR RGRLNRGNSR STWFVHDDLI 

       190        200        210        220        230        240 
DILGYGDYVF WKIPISMLPL NTQGIPHAAM DWYQASVFKE AVQGHTHIVS VSTADVLIMC 

       250        260        270        280        290        300 
KDLITCRFNT TLISKIAEIE DPVCSDYPNF KIVSMLYQSG DYLLSILGSD GYKIIKFLEP 

       310        320        330        340        350        360 
LCLAKIQLCS KYTERKGRFL TQMHLAVNHT LEEITEMRAL KPSQAQKIRE FHRTLIRLEM 

       370        380        390        400        410        420 
TPQQLCELFS IQKHWGHPVL HSETAIQKVK KHATVLKALR PIVIFETYCV FKYSIAKHYF 

       430        440        450        460        470        480 
DSQGSWYSVT SDRNLTPGLN SYIKRNQFPP LPMIKELLWE FYHLDHPPLF STKIISDLSI 

       490        500        510        520        530        540 
FIKDRATAVE RTCWDAVFEP NVLGYNPPHK FSTKRVPEQF LEQENFSIEN VLSYAQKLEY 

       550        560        570        580        590        600 
LLPQYRNFSF SLKEKELNVG RTFGKLPYPT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 

       610        620        630        640        650        660 
QKESLLHQAS WHHTSDDFGE HATVRGSSFV TDLEKYNLAF RYEFTAPFIE YCNRCYGVKN 

       670        680        690        700        710        720 
VFNWMHYTIP QCYMHVSDYY NPPHNLTLEN RDNPPEGPSS YRGHMGGIEG LQQKLWTSIS 

       730        740        750        760        770        780 
CAQISLVEIK TGFKLRSAVM GDNQCITVLS VFPLETDADE QEQSAEDNAA RVAASLAKVT 

       790        800        810        820        830        840 
SACGIFLKPD ETFVHSGFIY FGKKQYLNGV QLPQSLKTAT RMAPLSDAIF DDLQGTLASI 

       850        860        870        880        890        900 
GTAFERSISE TRHIFPCRIT AAFHTFFSVR ILQYHHLGFN KGFDLGQLTL GKPLDFGTIS 

       910        920        930        940        950        960 
LALAVPQVLG GLSFLNPEKC FYRNLGDPVT SGLFQLKTYL RMIEMDDLFL PLIAKNPGNC 

       970        980        990       1000       1010       1020 
TAIDFVLNPS GLNVPGSQDL TSFLRQIVRR TITLSAKNKL INTLFHASAD FEDEMVCKWL 

      1030       1040       1050       1060       1070       1080 
LSSTPVMSRF AADIFSRTPS GKRLQILGYL EGTRTLLASR IINNNTETPV LDRLRKITLQ 

      1090       1100       1110       1120       1130       1140 
RWSLWFSYLD HCDNILAEAL TQITCTVDLA QILREYSWAH ILEGRPLIGA TLPCMIEQFK 

      1150       1160       1170       1180       1190       1200 
VFWLKPYEQC PQCSNAKQPG GKPFVSVAVK KHIVSAWPNA SRLSWTIGDG IPYIGSRTED 

      1210       1220       1230       1240       1250       1260 
KIGQPAIKPK CPSAALREAI ELASRLTWVT QGSSNSDLLI KPFLEARVNL SVQEILQMTP 

      1270       1280       1290       1300       1310       1320 
SHYSGNIVHR YNDQYSPHSF MANRMSNSAT RLIVSTNTLG EFSGGGQSAR DSNIIFQNVI 

      1330       1340       1350       1360       1370       1380 
NYAVALFDIK FRNTEATDIQ YNRAHLHLTK CCTREVPAQY LTYTSTLDLD LTRYRENELI 

      1390       1400       1410       1420       1430       1440 
YDNNPLKGGL NCNISFDNPF FQGKQLNIIE DDLIRLPHLS GWELAKTIMQ SIISDSNNSS 

      1450       1460       1470       1480       1490       1500 
TDPISSGETR SFTTHFLTYP KIGLLYSFGA FVSYYLGNTI LRTKKLTLDN FLYYLTTQIH 

      1510       1520       1530       1540       1550       1560 
NLPHRSLRIL KPTFKHASVM SRLMSIDPHF SIYIGGAAGD RGLSDAARLF LRTSISSFLT 

      1570       1580       1590       1600       1610       1620 
FVKEWIINRG TIVPLWIVYP LEGQNPTPVN NFLYQIVELL VHDSSRQQAL KTTISDHVHP 

      1630       1640       1650       1660       1670       1680 
HDNLVYTCKS TASNFFHASL AYWRSRHRNS NRKYLARDSS TRSSTNNSDG HIERSQEQTT 

      1690       1700       1710       1720       1730       1740 
RDPHDGTERN LVLQMSHEIK RTTIPQENTH QGPSFQSFLS YSACGTANPK LNFDRSRHNV 

      1750       1760       1770       1780       1790       1800 
KSQDHNSASK REGHQIISHR LVLPFFTLSQ GTRQLTSSNE SQTQDEISKY LRQLRSVIDT 

      1810       1820       1830       1840       1850       1860 
TVYCRFTGIV SSMHYKLDEV LWEIESFKSA VTLAEGEGAG ALLLIQKYQV KTLFFNTLAT 

      1870       1880       1890       1900       1910       1920 
ESSIESEIVS GMTTPRMLLP VMSKFHNDQI EIILNNSASQ ITDITNPTWF KDQRARLPKQ 

      1930       1940       1950       1960       1970       1980 
VEVITMDAET TENINRSKLY EAVYKLILHH IDPSVLKAVV LKVFLSDTEG MLWLNDNLAP 

      1990       2000       2010       2020       2030       2040 
FFATGYLIKP ITSSARSSEW YLCLTNFLST TRKMPHQNHL SCKQVILTAL QLQIQRSPYW 

      2050       2060       2070       2080       2090       2100 
LSHLTQYADC DLHLSYIRLG FPSLEKVLYH RYNLVDSKRG PLVSITQHLA HLRAEIRELT 

      2110       2120       2130       2140       2150       2160 
NDYNQQRQSR TQTYHFIRTA KGRITKLVND YLKFFLIVQA LKHNGTWQAE FKKLPELISV 

      2170       2180       2190       2200       2210 
CNRFYHIRDC NCEERFLVQT LYLHRMQDSE VKLIERLTGL LSLFPDGLYR FD

Q6V1Q2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools