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UniProtKB/Swiss-Prot entry Q6P073


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UB2J2_MOUSE
Primary accession number Q6P073
Secondary accession numbers Q8C6A1 Q91Y64 Q9CWY5 Q9DC18 Q9QX58
Integrated into Swiss-Prot on September 13, 2004
Sequence was last modified on July 5, 2004 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 42)
Name and origin of the protein
Protein name Ubiquitin-conjugating enzyme E2 J2
Synonyms EC 6.3.2.19
Non-canonical ubiquitin-conjugating enzyme 2
NCUBE2
Gene name
Name: Ube2j2
Synonyms: Ncube2
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, AND SUBCELLULAR LOCATION.
STRAIN=C57BL/6J;
TISSUE=Fetus;
DOI=10.1074/jbc.M007640200; PubMed=11278356 [NCBI, ExPASy, EBI, Israel, Japan]
Tiwari S., Weissman A.M.;
"Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s).";
J. Biol. Chem. 276:16193-16200(2001).
[2]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, AND SUBCELLULAR LOCATION.
PubMed=12082160 [NCBI, ExPASy, EBI, Israel, Japan]
Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T.;
"A role for mammalian Ubc6 homologues in ER-associated protein degradation.";
J. Cell Sci. 115:3007-3014(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Lung, and Skin;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Thymus;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF296656; AAK52607.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U93242; AAF21504.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK004626; BAB23421.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK010302; BAB26835.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK076265; BAC36280.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC065779; AAH65779.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001034246.1; -.
NP_001034247.1; -.
NP_001034248.1; -.
NP_067377.4; -.
UniGene Mm.371673
3D structure databases
HSSP P34477; 1PZV. [HSSP ENTRY / PDB]
SMR Q6P073; 11-171.
ModBase Q6P073.
Organism-specific databases
MGI MGI:2153608; Ube2j2.
Gene expression databases
ArrayExpress Q6P073; -.
GermOnline ENSMUSG00000023286; Mus musculus.
Ontologies
GO
GO:0005783; Cellular component: endoplasmic reticulum (inferred from direct assay from MGI).
QuickGo view.
Family and domain databases
InterPro IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.
Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
PANTHER PTHR11621; UBQ-conjugat_E2; 1.
Pfam PF00179; UQ_con; 1.
Pfam graphical view of domain structure.
ProDom PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00212; UBCc; 1.
SMART graphical view of domain structure.
PROSITE PS00183; UBIQUITIN_CONJUGAT_1; FALSE_NEG.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q6P073.
Genome annotation databases
Ensembl ENSMUSG00000023286; Mus musculus. [Contig view]
GeneID 140499; -.
KEGG mmu:140499; -.
Phylogenomic databases
HOGENOM Q6P073; -.
HOVERGEN Q6P073; -.
Other
SOURCE Ube2j2; Mus musculus.
ProtoNet Q6P073.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Ligase; Membrane; Transmembrane; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   259  259     Ubiquitin-conjugating enzyme E2 J2. PRO_0000082597
TOPO_DOM   1   226  226     Cytoplasmic (Potential). 
TRANSMEM   227   247  21     Anchor for type IV membrane protein (Potential). 
TOPO_DOM   248   259  12     Lumenal (Potential). 
ACT_SITE   94    94        Glycyl thioester intermediate. 
MUTAGEN   94    94        C->S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. 
CONFLICT   25    25        K -> T (in Ref. 3; BAC36280). 
CONFLICT   32    32        I -> M (in Ref. 3; BAC36280). 
CONFLICT   49    49        R -> G (in Ref. 3; BAC36280). 
CONFLICT   80    80        M -> I (in Ref. 3; BAB26835). 
CONFLICT   84    84        N -> H (in Ref. 3; BAB26835). 
CONFLICT   87    87        F -> I (in Ref. 3; BAB26835). 
CONFLICT   133   133        E -> K (in Ref. 3; BAB23421). 
CONFLICT   150   150        N -> I (in Ref. 3; BAB26835). 
CONFLICT   157   157        C -> G (in Ref. 3; BAC36280). 
CONFLICT   162   162        E -> K (in Ref. 3; BAB23421). 
CONFLICT   186   186        L -> W (in Ref. 3; BAC36280). 
CONFLICT   217   218        AG -> GW (in Ref. 2; AAF21504). 
CONFLICT   237   237        F -> L (in Ref. 3; BAC36280). 
CONFLICT   246   246        A -> P (in Ref. 1; AAK52607). 
Sequence information
Length: 259 AA [This is the length of the unprocessed precursor] Molecular weight: 28954 Da [This is the MW of the unprocessed precursor] CRC64: 0C539BC0ACEAC7C7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSNNSNKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 

        70         80         90        100        110        120 
YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 

       130        140        150        160        170        180 
FMVEKGPTLG SIETSDFTKK QLAAQSLVFN LKDKVFCELF PEVVEEIKQK QKAQDELSNR 

       190        200        210        220        230        240 
PQNLPLPDVV PDGELHRGQH GIQLLNGHAP AAGPNLAGLP QANRHHGLLG GALANLFVIV 

       250 
GFAAFAYTVK YVLRSIAQE 

Q6P073 in FASTA format

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