NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Brown Norway;
DOI=10.1101/gr.1987704; PubMed=15060004 [NCBI, ExPASy, EBI, Israel, Japan]
Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.;
"The genomic sequence and comparative analysis of the rat major histocompatibility complex.";
Genome Res. 14:631-639(2004).

Comments

FUNCTION: Uses estradiol as its preferred substrate.
CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)⁺ = estrone + NAD(P)H.
CATALYTIC ACTIVITY: Testosterone + NAD⁺ = androst-4-ene-3,17-dione + NADH.
PATHWAY: Steroid metabolism; estrogen biosynthesis .
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BX883042; CAE83931.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_997694.1; -.

UniGene

Rn.137469

3D structure databases

ModBase

Q6MGB5.

Protein-protein interaction databases

IntAct

Q6MGB5; -.

Organism-specific databases

RGD

1303158; Hsd17b8.

Gene expression databases

ArrayExpress

Q6MGB5; -.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

Q6MGB5.

Genome annotation databases

Ensembl

ENSRNOG00000000466; Rattus norvegicus. [Contig view]

GeneID

361802; -.

KEGG

rno:361802; -.

NMPDR

fig|10116.3.peg.17523; -.

Phylogenomic databases

HOVERGEN

Q6MGB5; -.

Other

ProtoNet

Q6MGB5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; Lipid synthesis; NAD; Oxidoreductase; Steroid biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 259`	`259`	`Estradiol 17-beta-dehydrogenase 8.`	`PRO_0000304877`
`NP_BIND`	`13 37`	`25`	`NAD (By similarity).`
`ACT_SITE`	`167 167`		`Proton acceptor (By similarity).`
`BINDING`	`154 154`		`Substrate (By similarity).`
`MOD_RES`	`66 66`		`N6-acetyllysine (By similarity).`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 26791 Da [This is the MW of the unprocessed precursor]

CRC64: 5E23ECCE525E6781 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASQLRLRSA LALVTGAGSG IGRAISVRLA AEGAAVAACD LDGAAAQDTV RLLGNPGSED 

        70         80         90        100        110        120 
REPRGKHAAF QADVSEGPAA KRLLEQVQAC FFRPPSVVVS CAGITRDEFL LHMSEEDWDR 

       130        140        150        160        170        180 
VIAVNLKGTF LVTQAAAQAL VSSGGRGSII NISSIVGKVG NIGQTNYASS KAGVIGLTQT 

       190        200        210        220        230        240 
AARELGRHGI RCNSVLPGFI ATPMTQKMPE KVKDKVTAMI PLGHMGDPED VADVVAFLAS 

       250 
EDSGYITGAS VEVSGGLFM

Q6MGB5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools