NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
DOI=10.1073/pnas.0401356101; PubMed=15184674 [NCBI, ExPASy, EBI, Israel, Japan]
Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W.;
"Genome sequence of Picrophilus torridus and its implications for life around pH 0.";
Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).

Comments

FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE017261; AAT43143.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_023336.1; -.

3D structure databases

ModBase

Q6L1K9.

Enzyme and pathway databases

BioCyc

PTOR263820:PTO0558-MON; -.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

TIGRFAMs

TIGR00354; polC; 1.

BLOCKS

Q6L1K9.

Genome annotation databases

GeneID

2844296; -.

GenomeReviews

AE017261_GR; PTO0558.

KEGG

pto:PTO0558; -.

NMPDR

fig|263820.1.peg.558; -.

Phylogenomic databases

HOGENOM

Q6L1K9; -.

Genome annotation databases

CMR

Q6L1K9; PTO0558.

Other

ProtoNet

Q6L1K9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 1080`	`1080`		`DNA polymerase II large subunit.`	`PRO_0000294699`

Sequence information

Length: 1080 AA [This is the length of the unprocessed precursor]

Molecular weight: 121576 Da [This is the MW of the unprocessed precursor]

CRC64: 02273EA4918062BC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIDHSLFDYN ERLRKETYEC YEIAKKARAM GLDVSDEVEI PIANDMAERV EELIHISGIA 

        70         80         90        100        110        120 
GEIRELSKSM SREELSLHIS KKVASLLKDN RVEALDKAVR VGLAILTEGI LVAPLEGIKD 

       130        140        150        160        170        180 
VKINKNDDGT EYVSIVYSGP IRSAGGTAQA LSVLIADIVR RELNIGSFSP TDDEIERYIE 

       190        200        210        220        230        240 
EVEAYNRLKH LQYMPTPDEI RLVLKNSPVM IDGEGSEEEE VSGHRDMKRI TTNRIRGGMC 

       250        260        270        280        290        300 
LVLCEGLIQK AKKVLKYTNV MHLNEWNILE NIGKNKSDEK TENKSEKYLK DIIAGRPVFG 

       310        320        330        340        350        360 
YPNRPGGFRL RYGRSRVSGL AAASINPVTM KILNDFIAIG SQIKVELPGK AAAITPCDSI 

       370        380        390        400        410        420 
DGPMVLTRSG DHIKVKSIEQ AEKIKNDIER ITDLGEILIA YGDFLENNRN LDLSPFTREW 

       430        440        450        460        470        480 
WEYYLNDELS IYKSRDPDQF DAVNISRKYK MPMFPGYDYF WHDITMEELN LLINAIANGI 

       490        500        510        520        530        540 
INDDSMMLDA SVSEILIKLG IEFKKHNNKL ILHEYYPLIV SCGFDLINEK IVKVSDERKN 

       550        560        570        580        590        600 
DVLGTVNALS GIEIKPRAPV RVGARLGRPE KAGDRKMKPK VHGLFPLMNY GGSTRSIINA 

       610        620        630        640        650        660 
ARSGSIMDIE LGARICRACG TETPFVRCPK CGMPTEDSDS EKKFKIDISK ALDDAALRVS 

       670        680        690        700        710        720 
TDIGTIKELK GVKKLMSRRS VIEPLEKAIL RAKHGISINK DGTCRYDMSD IPITHFKYNE 

       730        740        750        760        770        780 
ISLTRQRLME LGYSDSDINE IYPQDIIIPR DAASYLLNVS KFIDDLLVNY YGLGPYYMCN 

       790        800        810        820        830        840 
SEDDLIGHLV IGLAPHTSGG IVGRIIGFSD VNGCYAHPFF HAAKRRNCDG DEDSIMLLLD 

       850        860        870        880        890        900 
GLLNFSKKYL PSTTGGLMDA PLVLTLILDP EEIDKEALNV DTLQRYPLDF YIATEKNAPP 

       910        920        930        940        950        960 
ASIENMMKTM KVLIKDGRYT GISYSFDTGD ISYGTRLSAY KTIGSMEEKI EKQLGLARIL 

       970        980        990       1000       1010       1020 
RSVDENDVAA RVLSSHFLPD IYGNFRSFFT QEFRCTKCNA KYRRVPLSGR CLRCGSSNII 

      1030       1040       1050       1060       1070       1080 
LTIHHGSIVK YLNETKKVMN EYKLPDYLVF RINRLLEQIE STFDIENGND TTLDALIENE

Q6L1K9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools