ID   DDL_BARQU               Reviewed;         306 AA.
AC   Q6FZ74;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   04-NOV-2008, entry version 40.
DE   RecName: Full=D-alanine--D-alanine ligase;
DE            EC=6.3.2.4;
DE   AltName: Full=D-alanylalanine synthetase;
DE   AltName: Full=D-Ala-D-Ala ligase;
GN   Name=ddl; OrderedLocusNames=BQ08830;
OS   Bartonella quintana (Rochalimaea quintana).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=803;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Toulouse;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic
RT   derivative of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
CC   -!- FUNCTION: Cell wall formation (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC       alanyl-D-alanine.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; BX897700; CAF26362.1; -; Genomic_DNA.
DR   RefSeq; YP_032495.1; -.
DR   GeneID; 2867175; -.
DR   GenomeReviews; BX897700_GR; BQ08830.
DR   KEGG; bqu:BQ08830; -.
DR   NMPDR; fig|283165.1.peg.765; -.
DR   HOGENOM; Q6FZ74; -.
DR   BioCyc; BQUI283165:BQ08830-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_00047; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR005905; D_ala_D_ala.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   Pfam; PF01820; Dala_Dala_lig_N; 1.
DR   TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW   Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT   CHAIN         1    306       D-alanine--D-alanine ligase.
FT                                /FTId=PRO_1000030430.
FT   DOMAIN      102    300       ATP-grasp.
FT   NP_BIND     128    183       ATP (By similarity).
FT   METAL       253    253       Magnesium or manganese 1 (By similarity).
FT   METAL       267    267       Magnesium or manganese 1 (By similarity).
FT   METAL       267    267       Magnesium or manganese 2 (By similarity).
FT   METAL       269    269       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   306 AA;  33288 MW;  1ACFFD24DB7374BD CRC64;
     MKGEHVAVLM GGGSSERSVS LSSGTACADI LEARGYRVSR VDVDSHIASV LERLQPDVAF
     NALHGPFGED GCIQGVLEYL KIPYTHSGVM ASALAMDKGR AKIVVASVGV SVAPSCIMSR
     FAVGREHPME PPYVIKPLCE GSSFGVVIVK ENESAPPRNV VGAEWVYADE VIVEKYIPGR
     EFTCAVLGNE ALDVCEIFPD KHFQFYNYDS KYKSGGSLHI CPAQLSPNIY QNVQRMSLAA
     HQAIGCRGVS RSDFRFNEET GELVWLEINT QPGMTPTSLF PDIAKASGRT YGDIVQWMVE
     DASCMR
//