[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

CATALYTIC ACTIVITY: Isocitrate + NAD⁺ = 2-oxoglutarate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC079113; AAH79113.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_446033.1; -.

UniGene

Rn.1093

3D structure databases

ModBase

Q68FX0.

Organism-specific databases

RGD

621881; Idh3B.

Gene expression databases

ArrayExpress

Q68FX0; -.

Family and domain databases

InterPro

IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00175; mito_nad_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

Q68FX0.

Genome annotation databases

Ensembl

ENSRNOG00000007316; Rattus norvegicus. [Contig view]

GeneID

94173; -.

KEGG

rno:94173; -.

NMPDR

fig|10116.3.peg.19717; -.

Phylogenomic databases

HOVERGEN

Q68FX0; -.

Other

ProtoNet

Q68FX0.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 34`	`34`	`Mitochondrion (By similarity).`
`CHAIN`	`35 385`	`351`	`Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial.`	`PRO_0000014447`
`METAL`	`251 251`		`Magnesium or manganese (By similarity).`
`BINDING`	`133 133`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`251 251`		`Substrate (By similarity).`
`SITE`	`171 171`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`218 218`	`1`	`Critical for catalysis (By similarity).`

Sequence information

Length: 385 AA [This is the length of the unprocessed precursor]

Molecular weight: 42354 Da [This is the MW of the unprocessed precursor]

CRC64: DC91921B48EEED1E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAALSNVRWL TRAVLGARNS GAWRGLRTAA SAQAASQSQA QDVRVEGAFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAAAVPVEF KEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIYTPME 

       130        140        150        160        170        180 
YKGELASYDM QLRRKLDLFA NVVHVKSLPG YKTRHNNLDL VIIREQTEGE YSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RTKSQRIAKF AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL HPHGG

Q68FX0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools