Protein p19
Transmembrane protein 1A
Serine protease p27
     (p27)
     (EC 3.4.21.-)
Protein p20
RNA-directed RNA polymerase p57
     (p57)
     (EC 2.7.7.48)

Gene name

Name:

ORF1

From

Human astrovirus-1 (HAstV-1)

[TaxID: 12456]

Taxonomy

Viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; Mamastrovirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=8021608 [NCBI, ExPASy, EBI, Israel, Japan] Willcocks M.M., Brown T.D., Madeley C.R., Carter M.J.; "The complete sequence of a human astrovirus."; J. Gen. Virol. 75:1785-1788(1994).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=8254779 [NCBI, ExPASy, EBI, Israel, Japan] Lewis T.L., Greenberg H.B., Herrmann J.E., Smith L.S., Matsui S.M.; "Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein."; J. Virol. 68:77-83(1994).
[3]	NSP1A POLYPROTEIN PROCESSING. DOI=10.1128/JVI.76.4.2003-2008.2002; PubMed=11799197 [NCBI, ExPASy, EBI, Israel, Japan] Geigenmuller U., Chew T., Ginzton N., Matsui S.M.; "Processing of nonstructural protein 1a of human astrovirus."; J. Virol. 76:2003-2008(2002).

Comments

FUNCTION: Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by ribosomal frameshifting.

Name	nsp1ab
Isoform ID	Q67726-1
Note: Generated by a ribosomal frameshift at position 919.
This is the isoform sequence displayed in this entry.

Name	nsp1a
Isoform ID	P0C6K4-1
This isoform is stored in UniProtKB/Swiss-Prot entry P0C6K4.

PTM: Cleaved presumably by viral and host proteases.
SIMILARITY: Belongs to the astroviridae polyprotein 1AB family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z25771; CAA81033.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

C49529; C49529.

3D structure databases

ModBase

Q67726.

Family and domain databases

InterPro

IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Coiled coil; Hydrolase; Membrane; Nucleotide-binding; Nucleotidyltransferase; Protease; Ribosomal frameshifting; RNA replication; RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1436`	`1436`	`Non-structural polyprotein 1AB.`	`PRO_0000327271`
`CHAIN`	`1 175`	`175`	`Protein p19 (Potential).`	`PRO_0000327272`
`CHAIN`	`176 419`	`244`	`Transmembrane protein 1A (Potential).`	`PRO_0000327273`
`CHAIN`	`420 664`	`245`	`Serine protease p27 (Potential).`	`PRO_0000327274`
`CHAIN`	`665 933`	`269`	`Protein p20 (Potential).`	`PRO_0000327275`
`CHAIN`	`934 1436`	`503`	`RNA-directed RNA polymerase p57 (Potential).`	`PRO_0000327276`
`TRANSMEM`	`156 176`	`21`	`Potential.`
`TRANSMEM`	`239 259`	`21`	`Potential.`
`TRANSMEM`	`286 306`	`21`	`Potential.`
`TRANSMEM`	`313 333`	`21`	`Potential.`
`TRANSMEM`	`344 364`	`21`	`Potential.`
`DOMAIN`	`1181 1307`	`127`	`RdRp catalytic.`
`COILED`	`104 142`	`39`	`Potential.`
`COILED`	`587 620`	`34`	`Potential.`
`ACT_SITE`	`461 461`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`489 489`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`551 551`		`Charge relay system; for serine protease activity (By similarity).`
`SITE`	`175 176`	`2`	`Cleavage (Potential).`
`SITE`	`419 420`	`2`	`Cleavage (Potential).`
`SITE`	`664 665`	`2`	`Cleavage (Potential).`
`SITE`	`933 934`	`2`	`Cleavage (Potential).`

Sequence information

Length: 1436 AA [This is the length of the unprocessed precursor]

Molecular weight: 163874 Da [This is the MW of the unprocessed precursor]

CRC64: CEC41D44BCACD32F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAYGEPYYSS KPDKDFNFGS TMARRQMTPT MVTKLPKFVR NSPQAYDWIV RGLIFPTIGK 

        70         80         90        100        110        120 
TYFQRVVVIT GGLEDGTYGS FAFDGKEWVG IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ 

       130        140        150        160        170        180 
EEKATLALDV QFLQHENVRL KEMIPKPEPR KIQMKWIIMG AVLTFLSLIP GGYAHSQTNN 

       190        200        210        220        230        240 
TIFTDMIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRAHWLRTVF 

       250        260        270        280        290        300 
YYIHYYEMWN IFMFVLAIGT VMRSARPGTD LVTLATSHLS GFRMAVLPTI PFHTTMTLWV 

       310        320        330        340        350        360 
MNTLMVCYYF DNLLAITLAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT 

       370        380        390        400        410        420 
LTGTTTSLFV VILTCRFIRM ATVFIGTRFE IRDANGKVVA TVPTRIKNVA FDFFQKLKQS 

       430        440        450        460        470        480 
GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HVVGNNTFVN VCYEGLMYEA 

       490        500        510        520        530        540 
KVRYMPEKDI AFLTCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL 

       550        560        570        580        590        600 
SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PADFHPVKAP SQVELLKEEI 

       610        620        630        640        650        660 
ERLKAQLNSA TENATTVVTQ QPSAALEQKS VSDSDVVDLV RTAMEREMKV LRDEINGILA 

       670        680        690        700        710        720 
PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY 

       730        740        750        760        770        780 
PDYDDEDYYD EDDDGWGMVG DDVEFDYTEV INFDQAKPIP APRTTKQKIC PEPEVESQPL 

       790        800        810        820        830        840 
DLSQKKEKQS EYEQQVVKST KPQQLEHEQQ VVKPIKPQKS EPQPYSQTYG KAPIWESYDF 

       850        860        870        880        890        900 
DWDEDDAKFI LPAPHRLTKA DEIVLGSKIV KLRTIIETAI KTQNYSALPE AVFELDKAAY 

       910        920        930        940        950        960 
EAGLEGFLQR VKSKNKAPEK QGPKKLQRAP EDQGAQNYHS LDAWKLLLEP PRERRCVPAN 

       970        980        990       1000       1010       1020 
FPLLGHLPIN RPIFDDKKPR DDLLGLLPEP TWHAFEEYGP TTWGPQAFIK SFDKFFYAEP 

      1030       1040       1050       1060       1070       1080 
IDFFSEYPQL CAFADWATYR EFRYLEDTRV IHITATEKNT DSTPAYPKMN YFDTEENYLE 

      1090       1100       1110       1120       1130       1140 
AHGWAPYIRE FTRVYKGDKP EVLWYLFLKK EIIKEEKIRN SDIRQIVCAD PIYTRIGACL 

      1150       1160       1170       1180       1190       1200 
EAHQNALMKQ HTDTSVGQCG WSPMEGGFKK TMQRLVNKGN KHFIEFDWTR YDGTIPPALF 

      1210       1220       1230       1240       1250       1260 
KHIKEIRWNF INKDQREKYR HVHEWYVNNL LNRHVLLPSG EVTLQTRGNP SGQFSTTMDN 

      1270       1280       1290       1300       1310       1320 
NMVNFWLQAF EFAYFNGPDR DLWKTYDTVV YGDDRLSTTP SVPDDYEERV ITMYRDIFGM 

      1330       1340       1350       1360       1370       1380 
WVKPGKVICR DSIVGLSFCG FTVNENLEPV PTSPEKLMAS LLKPYKILPD LESLHGKLLC 

      1390       1400       1410       1420       1430 
YQLLAAFMAE DHPFKVYVEH CLSRTAKQLR DSGLPARLTE EQLHRIWRGG PKKCDG

Q67726 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools