RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Rabies virus (strain silver-haired bat-associated) (RABV) (SHBRV)

[TaxID: 445793]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.

Virus host

Mammalia [TaxID: 40674]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1073/pnas.0407289101; PubMed=15520387 [NCBI, ExPASy, EBI, Israel, Japan]
Faber M., Pulmanausahakul R., Nagao K., Prosniak M., Rice A.B., Koprowski H., Schnell M.J., Dietzschold B.;
"Identification of viral genomic elements responsible for rabies virus neuroinvasiveness.";
Proc. Natl. Acad. Sci. U.S.A. 101:16328-16332(2004).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviruses protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY705373; AAU11519.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q66T60.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2128`	`2128`	`Large structural protein.`	`PRO_0000294418`
`DOMAIN`	`612 800`	`189`	`RdRp catalytic.`
`REGION`	`1563 2128`	`566`	`Interaction with P protein (By similarity).`

Sequence information

Length: 2128 AA [This is the length of the unprocessed precursor]

Molecular weight: 243090 Da [This is the MW of the unprocessed precursor]

CRC64: A039BB2078B76EFD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMIDPGEVYD DPIDLIESEA EPKGNPTIPN ILRNSDYNLN SPLIEDPARL MLEWLKTGNR 

        70         80         90        100        110        120 
PLRMTLTDNC SRSHKVLKDY FKRVDLGSLK VGGAAAQSMI SLWLYGAHSE SNRSRKCIND 

       130        140        150        160        170        180 
LAQFYYKSSP IEKLLNCTLG NRGLKTPPEG VLSCLARVDY DKAFGRYLAN IYSSYLFFHV 

       190        200        210        220        230        240 
ITLYMNALDW DEEKTILALW RDITSIDTGK DLVKFKDQIW GLLIVTKDFV YSQSTGCLFD 

       250        260        270        280        290        300 
RNYTLMLKDL FLSRFNSLMI LLSPPEPRYS DDLISQLCQL YIAGDQVLSM CGNSGYEVIK 

       310        320        330        340        350        360 
ILEPYVVNSL VQRAEKFRPL IHSLGDFPTF IRDKVGQLEG TFGPSAKRFF KVLDQFDNIH 

       370        380        390        400        410        420 
DLVFVYGCYR HWGHPYIDYR KGLSKLYDQV HLKKVIDKSY QECLASDLAR RILRWGFDKY 

       430        440        450        460        470        480 
SKWYLDSRLL SRDHPLIPYI KTQTWPPRHI VDLVGDTWHK LPITQIFEIP ESMDPSEILD 

       490        500        510        520        530        540 
DKSHSFTRTR LASWLSENRG GPVPSEKVII TALSKPPVNP REFLKSIDLG GLPDEDLIIG 

       550        560        570        580        590        600 
LKPKERELKI EGRFFALMSW NLRLYFVITE KLLANYILPL FDALTMTDNL NKVFKKLIDR 

       610        620        630        640        650        660 
VTGQGLLDYS RVTYAFRLDY EKWNNHQRLE STEDVFSVLD QVFGLKRVFS RTHEFFQRSW 

       670        680        690        700        710        720 
IYYSDRSDLI GLWEDQIYCL DMSDGPTCWN GQDGGLEGLR QKGWSLVSLL MIDRESQTRN 

       730        740        750        760        770        780 
TRTKILAQGD NQVLCPTYML SPGLSREGLL YELESISRNA LSIYRAIEEG ASKLGLIIKK 

       790        800        810        820        830        840 
EETMCSYDFL IYGKTPLFRG NILVPESKRW ARVSCISNDQ IVNLANTMST VSTNALTVAQ 

       850        860        870        880        890        900 
HSQSLIKPMR DFLLMSVQAV FHYLLFSPIL KGRVYKILSA EGDNFLLAMS RIIYLDPSLG 

       910        920        930        940        950        960 
GVSGMSLGRF HIRQFSDPVS EGLSFWREIW LSSNESWIHA LCQEAGNPDL GERTLESFTR 

       970        980        990       1000       1010       1020 
LLEDPTTLNI KGGASPTILL KDAIRKALYD EVDEVENSEF REAILLSKTH RDNFILFLKS 

      1030       1040       1050       1060       1070       1080 
IEPLFPRFLS ELFSSSFLGI PESIIGLIQN SRTIRRQFRR SLSRTLEESF YNSETHGINR 

      1090       1100       1110       1120       1130       1140 
MTQTPQRVGR VWPCSSERAD LLREISWGRK VVGTTVPHPS EMLGLIPKSS ISCTCGAAGG 

      1150       1160       1170       1180       1190       1200 
GNPRISVSVL PSFDQSFFSR GPLKGYLGSS TSMSTQLFHA WEKVTNVHVV KRALSLKESI 

      1210       1220       1230       1240       1250       1260 
NWFITRNSNR AQTLIRNIMS LTGPDFPLEE APVFKRTGSA LHRFKSARYS EGGYSSVCPN 

      1270       1280       1290       1300       1310       1320 
LLSHISVSTD TMSDLTQDGR NYDFMFQPLM LYAQTWTSEL VQKDTRLKDS TFHWHLRCNK 

      1330       1340       1350       1360       1370       1380 
CIRPIDDMTL DTSQVFEFPD VSRRISRMVS GAVPHFRKLP DIRLRPGDFE SLSGKEKSRH 

      1390       1400       1410       1420       1430       1440 
IGSAQGLLYS ILVAIHDSGY NDGTIFPVNI YSKVSPRDYL RGLARGVLIG SSICFLTRMT 

      1450       1460       1470       1480       1490       1500 
NININRPLEL ISGVISYILL RLDNHPSLYI MLREPSLRGE IFSIPQKVPA AYPTTMKEGN 

      1510       1520       1530       1540       1550       1560 
RSILCYLQHV LRYEREAITA SPENDWLWIF SDFRSSKMTY LTLITYQSHL LLQRVDKNLS 

      1570       1580       1590       1600       1610       1620 
KSMRANLRQM SSLMRQVLGG HGEDTLESDE DIQRLLKDSL RRTRWVDQEV RHAARTMTGS 

      1630       1640       1650       1660       1670       1680 
YSPHRRVSRK AGCSEWVCSA QQVAVSTSAN PAPASELDIR TLSRRLQNPL ISGLRVVQWA 

      1690       1700       1710       1720       1730       1740 
TGAHYKLKPI LDDLNVFPSL CLVVGDGSGG ISRAVLNMFP DARLVFNSLL EVNDLMASGT 

      1750       1760       1770       1780       1790       1800 
HPLPPSAIMS GGDDIISRVI DFDSIWEKPS DLRNLTTWRY FQSVQEQVNM SYDLIICDAE 

      1810       1820       1830       1840       1850       1860 
VTDIASINRI TLLMSDFALS IDGPLYLVFK TYGTMLVNPD YRAIQHLSRA FPAVTGFITQ 

      1870       1880       1890       1900       1910       1920 
MTSSFSSELY LRFSKRGKFF RDAEYLTSST LREMSLVLFN CSSPKSEMQR ARSLNYQDLV 

      1930       1940       1950       1960       1970       1980 
RGFPDEVISN PYNEMIITLI DSDVESFLVH KMVDDLELQR GTLSKVSIII AIMIVFSNRV 

      1990       2000       2010       2020       2030       2040 
FNVSKPLTDP LFYPPFDPKI LRHFNICCST MMYLSTALGD VPSFARLHDL YNRPITYYFR 

      2050       2060       2070       2080       2090       2100 
KQVIRGNIYL SWSWSDDTSV FKRVACNSSL SLSSHWIRLI YKIVKTTRLV GSIEDLSGEI 

      2110       2120 
EKHLRGYNRW ITLDDIRSRS SLLDYSCL

Q66T60 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools