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UniProtKB/Swiss-Prot entry Q60555

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name 3BHS1_MESAU
Primary accession number Q60555
Secondary accession number Q60556
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 57)
Name and origin of the protein
Protein name 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Synonym 3-beta-HSD I
Includes 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
     (EC 1.1.1.145)
     (3-beta-hydroxy-5-ene steroid dehydrogenase)
     (Progesterone reductase)
Steroid Delta-isomerase
     (EC 5.3.3.1)
     (Delta-5-3-ketosteroid isomerase)
Gene name
Name: HSD3B1
From
Mesocricetus auratus (Golden hamster) [TaxID: 10036] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Cricetidae; Cricetinae; Mesocricetus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal gland;
DOI=10.1016/0960-0760(95)00197-2; PubMed=8547173 [NCBI, ExPASy, EBI, Israel, Japan]
Rogerson F.M., Lehoux J.-G., Mason J.I.;
"Expression and characterization of isoforms of 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase in the hamster.";
J. Steroid Biochem. Mol. Biol. 55:481-487(1995).
Comments
  • FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes hormonal steroids. Also able to act as 3-keto-reductase reducing 5-alpha-dihydrotestosterone to the corresponding 3-beta-androstan-3-beta,17-beta-diol.
  • CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH.
  • CATALYTIC ACTIVITY: A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.
  • PATHWAY: Lipid metabolism; steroid biosynthesis.
  • SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
  • TISSUE SPECIFICITY: High levels in adrenal, kidney and male liver. Low levels in female liver.
  • SIMILARITY: Belongs to the 3-beta-HSD family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L38709; AAB52546.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
ModBase Q60555.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0031966; Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR002225; 3Beta_OHSteriod_DHase/Estase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF01073; 3Beta_HSD; 1.
Pfam graphical view of domain structure.
BLOCKS Q60555.
Phylogenomic databases
HOVERGEN Q60555; -.
Other
ProtoNet Q60555.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion; Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   373  372     3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1. PRO_0000087777
TRANSMEM   288   308  21     Potential. 
Sequence information
Length: 373 AA [This is the length of the unprocessed precursor] Molecular weight: 41754 Da [This is the MW of the unprocessed precursor] CRC64: 4975BC9F2D93D159 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MPGWSCLVTG AGGFLGQRII RMLVQEKELQ EVRALDKVFR PETREEFCKL QTKTKVTVLE 

        70         80         90        100        110        120 
GDILDAQCLR RACQGISVVI HTAAAIDVFG AIPRQTIIDI NLKGTLNLLE ACVQASVPAF 

       130        140        150        160        170        180 
IYTSSIDVAG PNSYKEIVLN GHEEQQHEST WSDPYPYSKK MAEKAVLAAN GSSLKNGGTL 

       190        200        210        220        230        240 
HTCALRPMYI YGEKSPLISV TIIRAVKNSG ILDVTGKFST VNPVYVNNAA WAHILAARGL 

       250        260        270        280        290        300 
QDPRKSPNIQ GQFYYISDDT PHQSYDDLNY VLSKDWGLRP DSSWRPPVAL LYWLGFLLEL 

       310        320        330        340        350        360 
VSFLLRPVYN YQPPFNRHLV TLSNTVFTFS YKKAQRDLGY EPLVGWEEAR ENTSEWIGSL 

       370 
VEQHKGTLNT KAQ
Q60555 in FASTA format

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