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- FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (By similarity).
- CATALYTIC ACTIVITY: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
- SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit (By similarity).
- SUBCELLULAR LOCATION: Cytoplasm.
- PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa (By similarity).
- PTM: S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol (By similarity).
- SIMILARITY: Belongs to the peptidase C14 family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 277 AA [This is the length of the unprocessed precursor] |
Molecular weight: 31612 Da [This is the MW of the unprocessed precursor] |
CRC64: 0BF3A4590A2828A3 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MENNETSVDS KSIKNFEVKT IHGSKSMDSG IYLDSSYKMD YPEMGVCIII NNKNFHKSTG
70 80 90 100 110 120
MTPRSGTDVD AAKLRETFMA LKYEVRNKND LTREEIVELM KNASKEDHSK RSSFVCVILS
130 140 150 160 170 180
HGDEGVIFGT DGPIDLKKLT SYFRGDYCRS LIGKPKLFII QACRGTELDC GIETDSGTED
190 200 210 220 230 240
DMTCQKIPVE ADFLYAYSTA PGYYSWRNPK DGSWFIQSLC SMLKLYAHKL EFMHILTRVN
250 260 270
RKVATEFESF SLDSTFHAKK QIPCIVSMLT KELYFYH
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Q60431 in FASTA format |
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ExPASy Home page
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