[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1016/j.gene.2005.10.043; PubMed=16413149 [NCBI, ExPASy, EBI, Israel, Japan] Gil R., Sabater-Munoz B., Perez-Brocal V., Silva F.J., Latorre A.; "Plasmids in the aphid endosymbiont Buchnera aphidicola with the smallest genomes. A puzzling evolutionary story."; Gene 370:17-25(2006).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1126/science.1130441; PubMed=17038625 [NCBI, ExPASy, EBI, Israel, Japan] Perez-Brocal V., Gil R., Ramos S., Lamelas A., Postigo M., Michelena J.M., Silva F.J., Moya A., Latorre A.; "A small microbial genome: the end of a long symbiotic relationship?"; Science 314:312-313(2006).

Comments

FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
CATALYTIC ACTIVITY: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD⁺ = 3-carboxy-4-methyl-2-oxopentanoate + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY438025; AAR99734.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q5WPZ9.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862;	Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0009098;	Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01033; -; 1.
PBIL [Tree]

InterPro

IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00169; leuB; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

Q5WPZ9.

Genome annotation databases

GenomeReviews

AY438025_GR; BCc_PL3.

CMR

Q5WPZ9; BCc_PL3.

Other

ProtoNet

Q5WPZ9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 367`	`367`	`3-isopropylmalate dehydrogenase.`	`PRO_0000274189`
`NP_BIND`	`80 93`	`14`	`NAD (By similarity).`
`NP_BIND`	`288 300`	`13`	`NAD (By similarity).`
`METAL`	`230 230`		`Magnesium or manganese (By similarity).`
`METAL`	`254 254`		`Magnesium or manganese (By similarity).`
`METAL`	`258 258`		`Magnesium or manganese (By similarity).`
`BINDING`	`101 101`		`Substrate (By similarity).`
`BINDING`	`111 111`		`Substrate (By similarity).`
`BINDING`	`140 140`		`Substrate (By similarity).`
`BINDING`	`230 230`		`Substrate (By similarity).`
`SITE`	`147 147`	`1`	`Important for catalysis (By similarity).`
`SITE`	`198 198`	`1`	`Important for catalysis (By similarity).`

Sequence information

Length: 367 AA [This is the length of the unprocessed precursor]

Molecular weight: 41114 Da [This is the MW of the unprocessed precursor]

CRC64: EE0B76D6D4B38690 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKKKEYRIA VLPGDGIGPE VMQEAYKILN VINKKFDFNI YTNEYDIGGI AIDKYGAPLP 

        70         80         90        100        110        120 
KKTLNGCIES HAILFGSIGG KEWTHLPADE QPERGGLLPL RKFFNLFVNL RPIKLHFSLK 

       130        140        150        160        170        180 
KLSPLKKEII QDGFDILCVR ELIGGIYFGL PKGKNTFDKK NISAFDTEIY STKEIEKIAD 

       190        200        210        220        230        240 
IAFQLSLKRK KKVFSIDKAN VLESSMLWRE VVIQVSKRYP NVELIHLYID NAAMQLIKNP 

       250        260        270        280        290        300 
NQFDVILCSN LFGDILSDEC AALTGSLGML PSASLNESFF GLFEPAGGSA PDLKGKNIAN 

       310        320        330        340        350        360 
PIAQILSLAL LFKYSLQLEK ISHLIELSVL KTLEKGYRTL DISNGKKYIT TDQMGNYIAK 


TLSELIN

Q5WPZ9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools