[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). TISSUE=Brain cortex; The German cDNA consortium; Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: Isocitrate + NAD⁺ = 2-oxoglutarate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	Q5R678-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	Q5R678-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_014518.

SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR858162; CAH90401.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR860617; CAH92738.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q5R678.

Family and domain databases

InterPro

IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00175; mito_nad_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

Q5R678.

Phylogenomic databases

HOVERGEN

Q5R678; -.

Other

ProtoNet

Q5R678.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 27`	`27`	`Mitochondrion (By similarity).`
`CHAIN`	`28 366`	`339`	`Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial.`	`PRO_0000014439`
`METAL`	`233 233`		`Magnesium or manganese (By similarity).`
`METAL`	`257 257`		`Magnesium or manganese (By similarity).`
`METAL`	`261 261`		`Magnesium or manganese (By similarity).`
`BINDING`	`115 115`		`Substrate (By similarity).`
`BINDING`	`125 125`		`Substrate (By similarity).`
`BINDING`	`146 146`		`Substrate (By similarity).`
`BINDING`	`233 233`		`Substrate (By similarity).`
`SITE`	`153 153`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`200 200`	`1`	`Critical for catalysis (By similarity).`
`VAR_SEQ`	`1 50`		`Missing (in isoform 2).`	`VSP_014518`

Sequence information

Length: 366 AA [This is the length of the unprocessed precursor]

Molecular weight: 39619 Da [This is the MW of the unprocessed precursor]

CRC64: C89B61DC234430DA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAGPAWISKV SRLLGAFHNP KQVTRGFTGG VQTVTLIPGD GIGPEISAAV MKIFDAAKAP 

        70         80         90        100        110        120 
IQWEERNVTA IQGPGGKWMI PSEAKESMDK NKMGLKGPLK TPIAAGHPSM NLLLRKTFDL 

       130        140        150        160        170        180 
YANVRPCVSI EGYKTPYTDV NIVTIRENTE GEYSGIEHVI VDGVVQSIKL ITEGASKRIA 

       190        200        210        220        230        240 
EFAFEYARNN HRSNVTAVHK ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV 

       250        260        270        280        290        300 
QDPSQFDVLV MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD 

       310        320        330        340        350        360 
MANPTALLLS AVMMLRHMGL FDHAARIEAA CFATIKDGKS LTKDLGGNAK CSDFTEEICR 


RVKDLD

Q5R678 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools