RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Taro vein chlorosis virus (TAVCV)

[TaxID: 303300]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus; unclassified Nucleorhabdovirus.

Virus host

Colocasia esculenta (Elephant's ear) (Taro) [TaxID: 4460]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1099/vir.0.80591-0; PubMed=15659770 [NCBI, ExPASy, EBI, Israel, Japan] Revill P., Trinh X., Dale J., Harding R.; "Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus."; J. Gen. Virol. 86:491-499(2005).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY674964; AAV92087.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_224083.1; -.

3D structure databases

ModBase

Q5GA85.

Ontologies

GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q5GA85.

Genome annotation databases

GeneID

5076497; -.

Other

ProtoNet

Q5GA85.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1928`	`1928`	`Large structural protein.`	`PRO_0000297841`
`DOMAIN`	`596 782`	`187`	`RdRp catalytic.`

Sequence information

Length: 1928 AA [This is the length of the unprocessed precursor]

Molecular weight: 217363 Da [This is the MW of the unprocessed precursor]

CRC64: 042E4E6FBC396429 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDSDYPDLDP EALTVLDSIR EGIQDEEEED NNDKILSGTG DYHLKSALRT LDDMTRHPIF 

        70         80         90        100        110        120 
NKEYQKAVRH FGISPTMMMT PTAVLKLTVS QTKINKAVGF LFGDILVRLD SLPWAVDCYD 

       130        140        150        160        170        180 
SIQAEIKTMH SHMMIFATPS WVEDVHNKVS SLVEYDHDAT LIWATVITLK NYLPAWREKG 

       190        200        210        220        230        240 
ASLLDWRSVQ YDPESEYLVM KVDRDFIIYV GSDICVMEIG KQTLWAPVPY ILNGADKVAE 

       250        260        270        280        290        300 
RYNVKYYCAL CDELDIPDRI SLEKLNQIIE VGDDCLQALG NKGYDIIGSY EALLAGIIQA 

       310        320        330        340        350        360 
RDNPQVIPDR ELLQRTTLND PGNTIGVTFL KRWDALMEDL NPEQIACAHG LYRIWGHPAV 

       370        380        390        400        410        420 
DILGGINKMR EVASIVKLPS SKILTDIGRQ FKEMFFTSYH SVHKHYPKHL IREYKSDSYI 

       430        440        450        460        470        480 
HECLKDNRTL NSKVLSYHFP DWDSVALEKN FEVPYSWNLV HNLKDKAISP SRSELYETLS 

       490        500        510        520        530        540 
TRNSIFGASN RRGILKSLTM ETVQLRAFLQ DVNDKGLPDN DKIIGVYPKE RELKIKARLF 

       550        560        570        580        590        600 
SLMSFKLRLY FVSTEALLGD KILKYFPQIT MSLDMLSMIK KMFRVSGQTT RGDDSVTVIF 

       610        620        630        640        650        660 
NLDFVKWNLQ MRKIICSPVF TQLGALFGMP NLFDITHDLF RESVIYLCSG EGDLRGDPVF 

       670        680        690        700        710        720 
GVAPDGVWSW TGDESGKEGL RQKGWTILTV VTIMLIAKRH HVDVSLMGGG DNQVLGITIG 

       730        740        750        760        770        780 
GMVRDSVGEL TQDSCKLAQC TIKRFTQDLI TTFGDLGLPL KASETWVSDS LFMYNKHMFY 

       790        800        810        820        830        840 
KGMPLRSPLK AVSRIFPLAN DSIMTLDNMI NNISSGVKAA CMKERHGIPL VFIKTMAYRR 

       850        860        870        880        890        900 
VAELSLIMHP LTVCFKKPEL PDHGIVARSG KKLKIPVTSK NLRQYFSLCT LGSSTMGHPG 

       910        920        930        940        950        960 
TLHLPDIIMR GFPDPLTSHL SFISEMRRYI VDPGLASVVD KLSHLSTSPT TEYAKLVEDP 

       970        980        990       1000       1010       1020 
TSINHDAPTH GLNEIRQMSR DFLMSTTLAT NPHLKSLFSL LDRGTEKDFY DALCSAQELD 

      1030       1040       1050       1060       1070       1080 
VKVLHEIAGA TLYGYTNGIA SRIDQTGTVR ALNENIDVLR RLALAETRYI GYLMARDTRE 

      1090       1100       1110       1120       1130       1140 
HDLKPSSCSR ITAQQYRDLS WRKPILGVTV PHPMEMCQIM SSTETIYHDA VVCWSDRVSG 

      1150       1160       1170       1180       1190       1200 
SEIYQSMGQG KIYQGSYTKE RFKATDIAAA YGNEDILVKA VRLQKLINWR YDEGSNFAKI 

      1210       1220       1230       1240       1250       1260 
ISLTLEAITD ANTEGFHRSK EEIKGEFDHR RGVTGDISGG IPNFLVTPTS HFSSTTSSWV 

      1270       1280       1290       1300       1310       1320 
SHSRGGKNEN IHFQSVLINL LYRAMVYRGS VPGLPEMIWY SKEKCSDCIT EIKDPDPIKT 

      1330       1340       1350       1360       1370       1380 
TPSLHTLPSA KGNPFAYIES VNVKLDYHHQ IEITKGMEEE YLINSLWDGQ NVSGEEESGL 

      1390       1400       1410       1420       1430       1440 
LLYLMLIGSR QISESFILLM RERINAATAL QYMLNRVILA RKLGLDSQFP IRSTSCVNLL 

      1450       1460       1470       1480       1490       1500 
LGTDDNILSC RDRFNIELLS GSWESGVSCD MSVLYRDDLL TASELHVNVY LQNVPLQLAL 

      1510       1520       1530       1540       1550       1560 
SRAASSTQLQ SCLECQAIVL DRPSQRELMR YLHWCCPYHT ANAPPRILRI HSEKLIKGIE 

      1570       1580       1590       1600       1610       1620 
LRTDNPLLVP YVCNPVTLER VEKVPVMVDS WELPVHMHST WDSILPQLYL TLKSLLSQVT 

      1630       1640       1650       1660       1670       1680 
ISSLIVDDDI TLINLAASVM LDLRRELPVY INVKGFSGTE INNKFDNLKL LPPNIRSSVS 

      1690       1700       1710       1720       1730       1740 
VYHENRSLIE ASAAVWLPEP SSVESVGADW LVLWGDTWRM GAGVPGHVLV TESKLSTGMA 

      1750       1760       1770       1780       1790       1800 
WVLHRDPLAS QSVLELCGAV QIWEKKALDW DMREGHCVPI QMNRTLACSR LGSRGVRWTM 

      1810       1820       1830       1840       1850       1860 
WSTAAGLDKV TRILRSKLLS LSGNPGSSYH WRKGCQKILK AYVLSLYAHC VDNMLEASGR 

      1870       1880       1890       1900       1910       1920 
LVGVSVHGSL SGIVPICDMH SSDRIQRAQY LFLKERCQGG PFILRNRLER RINLLSPVHD 


LLDGPSQS

Q5GA85 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools