[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 56884 / 366; DOI=10.1002/(SICI)1097-0061(199607)12:9<893::AID-YEA973>3.0.CO;2-I; PubMed=8840507 [NCBI, ExPASy, EBI, Israel, Japan] Andaluz E., Larriba G., Calderone R.; "A Candida albicans gene encoding a DNA ligase."; Yeast 12:893-898(1996).
[2]	SEQUENCE REVISION TO C-TERMINUS. Larriba G.; Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=SC5314; DOI=10.1073/pnas.0401648101; PubMed=15123810 [NCBI, ExPASy, EBI, Israel, Japan] Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.; "The diploid genome sequence of Candida albicans."; Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
[4]	FUNCTION, AND INDUCTION. DOI=10.1002/(SICI)1097-0061(19990915)15:12<1199::AID-YEA447>3.0.CO;2-S; PubMed=10487922 [NCBI, ExPASy, EBI, Israel, Japan] Andaluz E., Ciudad A., Rubio Coque J., Calderone R., Larriba G.; "Cell cycle regulation of a DNA ligase-encoding gene (CaLIG4) from Candida albicans."; Yeast 15:1199-1210(1999).
[5]	FUNCTION. DOI=10.1128/IAI.69.01.137-147.2001; PubMed=11119499 [NCBI, ExPASy, EBI, Israel, Japan] Andaluz E., Calderone R., Reyes G., Larriba G.; "Phenotypic analysis and virulence of Candida albicans LIG4 mutants."; Infect. Immun. 69:137-147(2001).
[6]	FUNCTION. PubMed=12702284 [NCBI, ExPASy, EBI, Israel, Japan] Andaluz E., Ciudad T., Larriba G.; "An evaluation of the role of LIG4 in genomic instability and adaptive mutagenesis in Candida albicans."; FEMS Yeast Res. 2:341-348(2002).

Comments

FUNCTION: Involved in ds DNA break (DSB) repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (NHEJ). Not required for the repair of DSBs induced by ionizing radiation or UV light. Has a important role in morphogenesis, positively affecting the capacity to form hyphae.
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: Cell cycle-regulated. Expression peaks in late G1 and during the morphological transition.
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 1 BRCT domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X95001; CAA64457.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AACQ01000088; EAK96245.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AACQ01000087; EAK96312.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P52496.

Organism-specific databases

CGD

CAL0001558; LIG4.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF00533; BRCT; 2.
PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 1.
PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 928`	`928`	`DNA ligase 4.`	`PRO_0000059584`
`DOMAIN`	`673 769`	`97`	`BRCT 1.`
`DOMAIN`	`821 927`	`107`	`BRCT 2.`
`ACT_SITE`	`304 304`		`N6-AMP-lysine intermediate (By similarity).`
`CONFLICT`	`531 534`		`VYYS -> FIIV (in Ref. 1; CAA64457).`
`CONFLICT`	`564 564`		`A -> S (in Ref. 1; CAA64457).`
`CONFLICT`	`698 698`		`T -> R (in Ref. 1; CAA64457).`
`CONFLICT`	`894 894`		`E -> K (in Ref. 1; CAA64457).`

Sequence information

Length: 928 AA [This is the length of the unprocessed precursor]

Molecular weight: 107997 Da [This is the MW of the unprocessed precursor]

CRC64: E15D5151593E462E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTYFLNDIRP PSPNDITPSF TLLTKELFDK LDGVRKESLG DFRTVTEKKA FIIKTFINTF 

        70         80         90        100        110        120 
RTHIGNDIYP SAKLIFPEKS GRIYFIKEVA LARLLIKMYK IPKESEDYIT LHDWNKSYQR 

       130        140        150        160        170        180 
SRRFSIDEKK IRDLPLQASR IISKRRPIVD KLEEYTVPQI NSSLDQLALE KVSQGQIDIL 

       190        200        210        220        230        240 
KPLFDNLSIP EVRWLIHILL NKSILTSMER FFFNTWHPDG YRVFSICNDL QKTLQFSTNP 

       250        260        270        280        290        300 
DLRLDPSQLA IHPCFKFKPQ LSERLTTSYK TLVKKLQRKH EMDPPYEKKF QELGLENKFY 

       310        320        330        340        350        360 
IEEKMDGDRM LLHKDGDSFK FFSRRLKDYS YLYGESFQFG ALTKFLAHAF AGNIQSVILD 

       370        380        390        400        410        420 
GEMVAYDYER NVILPFGTLK SSAIQESVRQ FTTIDQYEQQ TAYPFFLVFD ILFLNGKDLT 

       430        440        450        460        470        480 
NYPLFFRKNI LNRILRPIPN RFEVLDTRLG SSSEDIERAI REVVSSRCEG LVLKNVQSKY 

       490        500        510        520        530        540 
EIDGFRNPDW IKVKPEYLEK FGENLDLVVI GKSPAIKNSY MCGLKSVTDG VYYSFCTCAN 

       550        560        570        580        590        600 
GIEIEEFDKI ERLTHGKWIK TDVAMPPESL IKFGTKIPTF WIHPSDSLVL EIRARSIDTR 

       610        620        630        640        650        660 
AGTSYAVGST LHNNHCRKIR EDKSIDECVT LQEYTHIKAN YINDLNKAQT ALGKKREPVY 

       670        680        690        700        710        720 
SLDNESKLKK VKVESDLFSG IEFLIMSDKR EADGEVTTIE EMKAMVKQYG GKIVNSVDLA 

       730        740        750        760        770        780 
TNYQIMVITE RELPVSSQYL SKGIDLVKPI WIYECIKRGC VLQLEPYFIF ASKNWDNFNH 

       790        800        810        820        830        840 
MVDQYGDSYI IHHPLNIVVP KLSESELEDL RNGFDWGDLK PWIYLFKGLS FYVCGNNLSA 

       850        860        870        880        890        900 
RFLKERIERF SGDLSKHFIE CCYIVIPDNH SRPLMLREID KMSNQISREM VIDENGGSSR 

       910        920 
IPHFVTEAFV QASIKMNYIP DPDDYKFR

P52496 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools