NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DG302;
PubMed=7539419 [NCBI, ExPASy, EBI, Israel, Japan]
Garmyn D., Ferain T., Bernard N., Hols P., Delplace B., Delcour J.;
"Pediococcus acidilactici ldhD gene: cloning, nucleotide sequence, and transcriptional analysis.";
J. Bacteriol. 177:3427-3437(1995).

Comments

FUNCTION: Has both D-lactate and D-glycerate dehydrogenase activities. Equally active on pyruvate and hydroxypyruvate.
CATALYTIC ACTIVITY: (R)-lactate + NAD⁺ = pyruvate + NADH.
SUBUNIT: Homodimer.
SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X70925; CAA50275.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P30901; 2DLD. [HSSP ENTRY / PDB]

ModBase

Q59642.

Family and domain databases

InterPro

IPR006139; D-isomer_2_OHA_DHase.
IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00389; 2-Hacid_dh; 1.
PF02826; 2-Hacid_dh_C; 1.
Pfam graphical view of domain structure.

PROSITE

PS00065; D_2_HYDROXYACID_DH_1; 1.
PS00670; D_2_HYDROXYACID_DH_2; 1.
PS00671; D_2_HYDROXYACID_DH_3; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 331`	`331`	`D-lactate dehydrogenase.`	`PRO_0000075958`
`ACT_SITE`	`234 234`		`By similarity.`
`ACT_SITE`	`263 263`		`By similarity.`
`ACT_SITE`	`295 295`		`Proton donor (By similarity).`

Sequence information

Length: 331 AA [This is the length of the unprocessed precursor]

Molecular weight: 37210 Da [This is the MW of the unprocessed precursor]

CRC64: C2E6653EC661AEB8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKIIAYGIRD DEKPYLDEWV TKNHIEVKAV PDLLDSSNID LAKDYDGVVA YQQKPYTADL 

        70         80         90        100        110        120 
FDKMHEFGIH AFSLRNVGLD NVPADALKKN DIKISNVPAY SPRAIAELSV TQLLALLRKI 

       130        140        150        160        170        180 
PEFEYKMAHG DYRWEPDIGL ELNQMTVGVI GTGRIGRAAI DIFKPFGAKV IAYDVFRNPA 

       190        200        210        220        230        240 
LEKEGMYVDT LEELYQQANV ITLHVPALKD NYHMLDEKAF GQMQDGTFIL NFARGTLVDT 

       250        260        270        280        290        300 
PALLKALDSG KVAGAALDTY ENEVGIFDVD HGDQPIDDPV FNDLMSRRNV MITPHAAFYT 

       310        320        330 
RPAVKNMVQI ALDNNRDLIE KNSSKNEVKF E

Q59642 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools