RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Lettuce necrotic yellows virus (LNYV)

[TaxID: 32612]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Cytorhabdovirus.

Virus hosts

Embergeria [TaxID: 43191]
Lactuca sativa (Garden lettuce) [TaxID: 4236]
Reichardia tingitana [TaxID: 43208]
Sonchus hydrophilus [TaxID: 255580]
Sonchus oleraceus (Common sowthistle) [TaxID: 50207]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=318;
DOI=10.1016/j.virusres.2005.10.024; PubMed=16313992 [NCBI, ExPASy, EBI, Israel, Japan]
Dietzgen R.G., Callaghan B., Wetzel T., Dale J.L.;
"Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus.";
Virus Res. 118:16-22(2006).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ746199; CAG34091.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ867584; CAI30426.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_425092.1; -.

3D structure databases

ModBase

Q4W382.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q4W382.

Genome annotation databases

GeneID

3844362; -.

Other

ProtoNet

Q4W382.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2068`	`2068`	`Large structural protein.`	`PRO_0000297837`
`DOMAIN`	`564 750`	`187`	`RdRp catalytic.`

Sequence information

Length: 2068 AA [This is the length of the unprocessed precursor]

Molecular weight: 236583 Da [This is the MW of the unprocessed precursor]

CRC64: 45AD5205F9A1B6EE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLWNEETTP KKNAYDSLPD YHLQNPLYAI TDQLSMLKRG KRLNYRLTSS YKLMKSQSTD 

        70         80         90        100        110        120 
IKEGDPILLK EWARNWFSET ELYVVDQTLS DCENRLSLDE TDDLHFDTHL LRESVRMWRC 

       130        140        150        160        170        180 
EFPHDDWVRM RGMQNLLIVM NAISSRRPPP PRHTCIIPGL SKITVEGGVV LVTSSLLGFQ 

       190        200        210        220        230        240 
PEGDKETTVF AADWVRAVSD VYTERFLVLS GAILGRCLSD EHYPQVLDLE FIINWGDTVL 

       250        260        270        280        290        300 
RRKGNKGFKL LKAYEALVLG VVQGKSESDF IDPDRFLRNT LNDIIDDDKD LGAYAVLLIQ 

       310        320        330        340        350        360 
RLQQIDSAHH LIQLFGLHRI WGHPLVDPAK GMEKMIIIGQ KDIVRETSRP EVMGVHFKKL 

       370        380        390        400        410        420 
MAQGYREKHG VYPNVKGNGE LETLIKNNHD WKEVGSLDND NHWRLLRFDK TFSIPESFNL 

       430        440        450        460        470        480 
SMIVADKSVS PTLSELKENI LRKKTVMNQE LRRGVLRWIN HDSIDPREFL EQVNDKKFPD 

       490        500        510        520        530        540 
DHKIIGLRSK EREMNPTPRM FALMSHLMRV YVVITESMLS EHILPYFPQI TMTDSQLDLT 

       550        560        570        580        590        600 
KKTYSTVKNQ AAKIRRSGAL YDTKTVCMSL DFEKWNGHMR KESTFHVFEA LGDLFGMENL 

       610        620        630        640        650        660 
YNETYDIFKD SYFYLADGSY VPSMDAQGNF TPEPPYSFTG HKGGQEGLRQ KGWTIFTVVC 

       670        680        690        700        710        720 
LDWICRKHNC TYKIMGMGDN QVLQLTMYTY RVDASGKATE RGREDMRRVL FGLFDDLLDV 

       730        740        750        760        770        780 
FSDLGLPLKP LETWISEDLF VYGKYPVLKG VPLSMDLKKI MRIFPFSNQE TMTIENALNT 

       790        800        810        820        830        840 
IAGNAQAATQ AAPFLGVSYL VGIFMISLCS HDMLVYHPLI AKGLREVLRE NRTWGLKFKG 

       850        860        870        880        890        900 
AHQVKTDIKG EMVDETNLRR LMMNVPRILG GYVSFNLYGL MMRGFPDPVS LAYSQLFSWG 

       910        920        930        940        950        960 
VPTGGSDQRD YMLRWLKPIF MPERSMRLLV EDVSSVNLLA PVTPTAGLRR VVEQYLTDGR 

       970        980        990       1000       1010       1020 
VIKNAEFRDL MMSRDNELED VISEHLCSGE HLHIRLIHDI MESTIFGYVK SITSKVTKSS 

      1030       1040       1050       1060       1070       1080 
TIVSLAIGKA KGDPLTRLMS DEENYFRFFM WRSVINPHYD IPACPTDLAK QVRRIGWGKE 

      1090       1100       1110       1120       1130       1140 
LIGVTVAYPW SFLKKTDCYE SGCLCTCDDG FISLFLPDSP VTPQEWDRSI GKNPPYLGSM 

      1150       1160       1170       1180       1190       1200 
TKEKVVISTG SKVYSGEPLV RRPINLMRVI GWFVPEESET AKIIMSCVSA VSDVNPLIFK 

      1210       1220       1230       1240       1250       1260 
GMTEGTSGSE IHRFRDTSLK HGALCSSNYL YSTRYHVSTD TFTRYAKGAQ NYDMLFQANL 

      1270       1280       1290       1300       1310       1320 
CAIVETTHQY VLKTNQSKEP QRKTHHYKQT CYSCINPLDE SFYDVKSSKL SQLIPSKKTN 

      1330       1340       1350       1360       1370       1380 
KYLYVPEAKI SMTLEHVPAK SWEFGTLSSD GFDQLSVNLR LQWLTDAVAD NVVIDILNPA 

      1390       1400       1410       1420       1430       1440 
GEESYTTTSL MDIKEHNRLF YLTIRPRDFY DQLCNRILIL AEWRCMSLSD WKTPTTEAIS 

      1450       1460       1470       1480       1490       1500 
RAAEAIIGDT PISRWYGVTG FFSWPSSMER YYVYPEIQEP DSIPVTALSA CRSVRNSLLG 

      1510       1520       1530       1540       1550       1560 
LLGSSRKFRG RNTRIFSEDA KASKLSLKLM VYDWVKKKKK CRACHREIGI MSAHQLSSTL 

      1570       1580       1590       1600       1610       1620 
PNSIICPKGH YVTQGLKDLD IMRSRVTLDS LRKCCSSDEI PTEPMEKKIT EWAPLTSTTC 

      1630       1640       1650       1660       1670       1680 
RTLFDSSSMR SELIPYSPTG IESSINVQPI PKSDLYKLIS LPTNAMYKYM EVISRNIEGI 

      1690       1700       1710       1720       1730       1740 
MNCKTAFVTG NGLGGTSKVL SNMWPGRIIT STLLDTGDAI PQVYPNCDKG SSSYARGTVI 

      1750       1760       1770       1780       1790       1800 
SDLMVTRVND VNHLLWGEDW KPVFQSYETD LCISDIEING ELNGESRQTM IATVTMAHDW 

      1810       1820       1830       1840       1850       1860 
KMVIMKDYIY NMREMENRLS ILLPVFKSLE LITCNSRQRV MPEVWWIMKA RRSSGKLLGY 

      1870       1880       1890       1900       1910       1920 
HRSVIRQIWD GVKEGINTAD WAMESVFSEI NRTIASTADM IAMTIRLKAF FSLPIVGSVL 

      1930       1940       1950       1960       1970       1980 
PYKGSYTRLL GYLQRGKKPE DISLITSDDG KRLYLSDYEK VRSVLFGLAV GMCSSATERD 

      1990       2000       2010       2020       2030       2040 
RMLDESEYWA IDWIPSGPHI WLPYLFKGVE RSTLIHVYDY IPMLTLIMKR ERLLFKSSSD 

      2050       2060 
IIEFKYTNNR DSCCFPITKT AKIKFNIK

Q4W382 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools