[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; International consortium for macaque cDNA sequencing and analysis; "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."; Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the mRNA cap methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB168817; BAE00921.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q4R7K1.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from sequence or structural similarity from UniProtKB).
GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0003723;	Molecular function: RNA binding (inferred from sequence or structural similarity from UniProtKB).
GO:0006370;	Biological process: mRNA capping (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR016899; mRNA_G-N7_MeTrfase.
IPR004971; Pox_MCEL.
Graphical view of domain structure.

Pfam

PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF028762; ABD1; 1.

BLOCKS

Q4R7K1.

Phylogenomic databases

HOVERGEN

Q4R7K1; -.

Other

ProtoNet

Q4R7K1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; mRNA capping; mRNA processing; Nucleus; Phosphoprotein; RNA-binding; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 476`	`476`	`mRNA cap guanine-N7 methyltransferase.`	`PRO_0000248322`
`REGION`	`176 177`	`2`	`mRNA cap binding (By similarity).`
`MOTIF`	`126 128`	`3`	`Nuclear localization signal (By similarity).`
`BINDING`	`180 180`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`205 205`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`
`BINDING`	`208 208`		`mRNA cap (By similarity).`
`BINDING`	`214 214`		`mRNA cap (By similarity).`
`BINDING`	`227 227`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`239 239`		`mRNA cap (By similarity).`
`BINDING`	`261 261`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`284 284`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`
`BINDING`	`288 288`		`mRNA cap (By similarity).`
`BINDING`	`370 370`		`mRNA cap (By similarity).`
`BINDING`	`467 467`		`mRNA cap (By similarity).`
`MOD_RES`	`24 24`		`Phosphoserine (By similarity).`
`MOD_RES`	`28 28`		`Phosphoserine (By similarity).`

Sequence information

Length: 476 AA [This is the length of the unprocessed precursor]

Molecular weight: 54797 Da [This is the MW of the unprocessed precursor]

CRC64: D22D4B4CE5A3A1E0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MANSTKAEEY EKMSVEQAKA SVNSEAESSF SINENTTASG TGLSGKTSVC RQVDTARKRK 

        70         80         90        100        110        120 
EFEDDLVKES SSCGEGTPSK KRKLDPEIVP EEKDCGDDEG NSKKRKRETE DVPKDEYSTG 

       130        140        150        160        170        180 
DGTQNKRKIA LEDVPEKQKN LEEGHSSAVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK 

       190        200        210        220        230        240 
SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSIKQCQQRY 

       250        260        270        280        290        300 
EDMKNRRDSE YIFSAEFITA DCSKELLIEK FRDPQMCFDI CSCQFVCHYS FESYEQADMM 

       310        320        330        340        350        360 
LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF 

       370        380        390        400        410        420 
NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP 

       430        440        450        460        470 
YPANESSKLV SERVDDYEHA AKYMKNSQVK LPLGTLSKSE WEATSIYLVF AFEKQQ

Q4R7K1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools