[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Parietal cortex; International consortium for macaque cDNA sequencing and analysis; "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."; Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in females. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation (By similarity).
CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)⁺ = estrone + NAD(P)H.
PATHWAY: Steroid metabolism; estrogen biosynthesis .
PATHWAY: Lipid metabolism; fatty acid biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
DOMAIN: The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins (By similarity).
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB169576; BAE01658.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q4R5G7.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004303;	Molecular function: estradiol 17-beta-dehydrogenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

Q4R5G7.

Phylogenomic databases

HOVERGEN

Q4R5G7; -.

Other

ProtoNet

Q4R5G7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 312`	`312`	`Estradiol 17-beta-dehydrogenase 12.`	`PRO_0000248369`
`TRANSMEM`	`4 24`	`21`	`Potential.`
`TRANSMEM`	`182 202`	`21`	`Potential.`
`TRANSMEM`	`271 291`	`21`	`Potential.`
`NP_BIND`	`50 79`	`30`	`NADP (By similarity).`
`MOTIF`	`308 312`	`5`	`Di-lysine motif (By similarity).`
`ACT_SITE`	`202 202`		`Proton acceptor (By similarity).`
`BINDING`	`189 189`		`Substrate (By similarity).`

Sequence information

Length: 312 AA [This is the length of the unprocessed precursor]

Molecular weight: 34555 Da [This is the MW of the unprocessed precursor]

CRC64: B7FB5351FC23724C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MESALPAAGF LYWVGAGTVA YLALRISYSL FTALRVWGVG NEAGVGPGLG EWAVVTGGTD 

        70         80         90        100        110        120 
GIGKSYAEEL AKRGMKVVLI SRSQDKLDQV SSEIKEKFKV ETRTIAVDFT LEDIYDKIKT 

       130        140        150        160        170        180 
GLAGLEIGIL VNNVGMSYEY PEYFLDVPDL DNVIKKMINI NILSVCKMTQ LVLPGMVERS 

       190        200        210        220        230        240 
KGAILNISSG SGMFPVPLLT IYSATKTFVD FFSQCLHEEY RSKGIFVQSV LPYFVATKLA 

       250        260        270        280        290        300 
KIRKPTLDKP TPETFVKSAI KTVGLQSRTN GYLIHVLMGW IISNLPSWIY LKIAMNMNKA 

       310 
TRVHYLKKIK KN

Q4R5G7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools