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UniProtKB/Swiss-Prot entry Q4G163


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FBX43_HUMAN
Primary accession number Q4G163
Secondary accession numbers None
Integrated into Swiss-Prot on July 25, 2006
Sequence was last modified on July 10, 2007 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 33)
Name and origin of the protein
Protein name F-box only protein 43
Synonym Endogenous meiotic inhibitor 2
Gene name
Name: FBXO43
Synonyms: EMI2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04406; PubMed=16421571 [NCBI, ExPASy, EBI, Israel, Japan]
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.;
"DNA sequence and analysis of human chromosome 8.";
Nature 439:331-335(2006).
[2]
NOMENCLATURE.
DOI=10.1101/gad.1255304; PubMed=15520277 [NCBI, ExPASy, EBI, Israel, Japan]
Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W.;
"Systematic analysis and nomenclature of mammalian F-box proteins.";
Genes Dev. 18:2573-2580(2004).
[3]
UBIQUITINATION, PHOSPHORYLATION AT SER-76 AND SER-334, AND MUTAGENESIS OF 75-ASP-SER-76 AND 333-ASP-SER-334.
DOI=10.1073/pnas.0501108102; PubMed=15753281 [NCBI, ExPASy, EBI, Israel, Japan]
Tung J.J., Hansen D.V., Ban K.H., Loktev A.V., Summers M.K., Adler J.R. III, Jackson P.K.;
"A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.";
Proc. Natl. Acad. Sci. U.S.A. 102:4318-4323(2005).
[4]
PHOSPHORYLATION AT THR-234.
DOI=10.1073/pnas.0509549102; PubMed=16407128 [NCBI, ExPASy, EBI, Israel, Japan]
Hansen D.V., Tung J.J., Jackson P.K.;
"CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit.";
Proc. Natl. Acad. Sci. U.S.A. 103:608-613(2006).
Comments
  • FUNCTION: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable).
  • PATHWAY: Protein modification; protein ubiquitination.
  • SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20 (By similarity).
  • PTM: Phosphorylated on Ser-76, Thr-234 and Ser-334 in reponse to calcium, which is a prerequisite for ubiquitination and proteasomal degradation (Probable).
  • PTM: Ubiquitinated in response to calcium, which promotes proteasomal degradation.
  • SIMILARITY: Contains 1 F-box domain.
  • SIMILARITY: Contains 1 IBR-type zinc finger.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC021590; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
IPI IPI00175439; -.
RefSeq NP_001025031.2; -.
UniGene Hs.339577
3D structure databases
ModBase Q4G163.
PTM databases
PhosphoSite Q4G163; -.
Organism-specific databases
GeneCards GC08M101214; -.
HGNC HGNC:28521; FBXO43.
GenAtlas FBXO43.
MIM 609110; gene. [NCBI / EBI]
PharmGKB PA134913061; -.
Gene expression databases
ArrayExpress Q4G163; -.
Bgee Q4G163; -.
CleanEx HS_FBXO43; -.
GermOnline ENSG00000156509; Homo sapiens.
Ontologies
GO
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0007126; Biological process: meiosis (inferred from electronic annotation from UniProtKB-KW).
GO:0019941; Biological process: modification-dependent protein catabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001810; F-box.
IPR002867; Znf_C6HC.
Graphical view of domain structure.
Pfam PF00646; F-box; 1.
PF01485; IBR; 1.
Pfam graphical view of domain structure.
SMART SM00647; IBR; 1.
SMART graphical view of domain structure.
PROSITE PS50181; FBOX; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE Q4G163; -.
Genome annotation databases
Ensembl ENSG00000156509; Homo sapiens. [Contig view]
GeneID 286151; -.
KEGG hsa:286151; -.
Phylogenomic databases
HOGENOM Q4G163; -.
HOVERGEN Q4G163; -.
Other
NextBio 96042; -.
SOURCE FBXO43; Homo sapiens.
ProtoNet Q4G163.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Meiosis; Metal-binding; Phosphoprotein; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   708  708     F-box only protein 43. PRO_0000247233
DOMAIN   490   547  58     F-box. 
ZN_FING   622   681  60     IBR-type. 
MOD_RES   76    76        Phosphoserine (Probable). 
MOD_RES   234   234        Phosphothreonine. 
MOD_RES   334   334        Phosphoserine (Probable). 
MOD_RES   503   503        Phosphotyrosine (By similarity). 
MUTAGEN   75    76        DS->AA: Impairs ubiquitination and degradation in response to calcium. 
MUTAGEN   333   334        DS->AA: Impairs ubiquitination and degradation in response to calcium. 
Sequence information
Length: 708 AA [This is the length of the unprocessed precursor] Molecular weight: 78402 Da [This is the MW of the unprocessed precursor] CRC64: E42A358AE7BD29D1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSFKDKDERI SCLEAYVTLT SKSSRFTDET EILKMSQRHS GQAGTEAGNG ADSPPIVNSK 

        70         80         90        100        110        120 
YSTFRDFCST SSFQDSGYNE LKSCSFDNID KEYLGKKEKG PTLLYEHPET SGLGLTHPLE 

       130        140        150        160        170        180 
SPTQKKKCIL PRKEKDKTPE LCETPKISGK KCLPRRRLNV SFALLKGDFE SQNSSLESSI 

       190        200        210        220        230        240 
SQVINLEKNI PSSASGFSRA NNFSPLVTST LKTEEVTSCS QKLRLNFSQQ KTSTIDDSKD 

       250        260        270        280        290        300 
DCSLFEVECI SPIQGNNFKD SITHDFSDSS LCINDENACP ELLGSSVSGT TCGTDEDIFV 

       310        320        330        340        350        360 
TPISNLVANI RFNASQILSP SPEVRGSIST PEDSGFNSLS LEKSEDSLSD QEGSFQELLQ 

       370        380        390        400        410        420 
KHKGTPKVGD TIRKTRHLGR SRRLSTLREQ SSQSETEEEK QIVHPDSEKR AAAASAISEG 

       430        440        450        460        470        480 
QLSSDESGDL TFSLKNLSKT PALQLVHELF MKSKRKRLQE NSGHEFLEQG DGEKIAVLQC 

       490        500        510        520        530        540 
ILAGLIGKKM GIEKLDILTE LKYRNLKHIL AMVLESLTAE SLCSVWKVSR NWREIVVQDK 

       550        560        570        580        590        600 
NANRRRKFYI TQLKTDSEGA VLNVEDAATR LQLLNRSALR SVQAQARIPG SQREQGSTLS 

       610        620        630        640        650        660 
PWGEVLTPLA SSSVTHLSSK QEEYVKVAKT LFTDEALKPC PRCQSPAKYQ PYKKRGLCSR 

       670        680        690        700 
TACGFDFCVL CLCAYHGSEE CSRGAAKPRN RKDALPGSAQ SKRNLKRL 

Q4G163 in FASTA format

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