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UniProtKB/Swiss-Prot entry Q46E19

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DP2L_METBF
Primary accession number Q46E19
Secondary accession numbers None
Integrated into Swiss-Prot on July 10, 2007
Sequence was last modified on September 13, 2005 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 20)
Name and origin of the protein
Protein name DNA polymerase II large subunit
Synonyms Pol II
EC 2.7.7.7
Gene name
Name: polC
OrderedLocusNames: Mbar_A0899
From
Methanosarcina barkeri (strain Fusaro / DSM 804) [TaxID: 269797] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.00810-06; PubMed=16980466 [NCBI, ExPASy, EBI, Israel, Japan]
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes.";
J. Bacteriol. 188:7922-7931(2006).
Comments
  • FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
  • CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
  • CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
  • SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
  • SIMILARITY: Belongs to the archaeal DNA polymerase II family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CP000099; AAZ69873.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq YP_304453.1; -.
3D structure databases
ModBase Q46E19.
Enzyme and pathway databases
BioCyc MBAR269797:MBAR_A0899-MON; -.
Ontologies
GO
GO:0008408; Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887; Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738; Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00324; -; 1.
PBIL [Tree]
InterPro IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.
Pfam PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF016275; PolC_DP2; 1.
TIGRFAMs TIGR00354; polC; 1.
BLOCKS Q46E19.
Genome annotation databases
GeneID 3625944; -.
GenomeReviews CP000099_GR; Mbar_A0899.
KEGG mba:Mbar_A0899; -.
NMPDR fig|269797.3.peg.460; -.
Phylogenomic databases
HOGENOM Q46E19; -.
Genome annotation databases
CMR Q46E19; Mbar_A0899.
Other
ProtoNet Q46E19.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   1146  1146     DNA polymerase II large subunit. PRO_0000294692
Sequence information
Length: 1146 AA [This is the length of the unprocessed precursor] Molecular weight: 128094 Da [This is the MW of the unprocessed precursor] CRC64: 867140FA2D6E386A [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGETIASKEM HEYFDELEAR LKKAIEIANT ARARGGDPKP VVEIPLAKDL ADRVENLIGV 

        70         80         90        100        110        120 
EGVAAKIRVL EEKMSREECA LEIGRQVAEG EVGNFATKKD AVEAAIRVSM AVITEGVVAA 

       130        140        150        160        170        180 
PIEGINKVEL GKNDDGSEYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPRPE 

       190        200        210        220        230        240 
EVERYVEEIV LYKRVASLQY MPSEDEIRLI VKNCPVCIDG DPTEEAEVEG HRNLERIGTN 

       250        260        270        280        290        300 
RIRGGMCLVL AEGLALKAPK VKKHVNKLNM DGWDWLDILI GGAKTGGDEE DEKKNKIKPK 

       310        320        330        340        350        360 
DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFAAAGI NPSTMVLLDD FITNGTQLKV 

       370        380        390        400        410        420 
ERPGKAAAMS AVDSIEGPTV RLYSGDLVRI DDIKEAYELR SQVEVIVDIG EILINYGDFL 

       430        440        450        460        470        480 
ENNHPLMPSP YVFEWWRYDY EAACSEIIPE DELKDPSSAL ALRLAEEYDV PLHPKFTYLW 

       490        500        510        520        530        540 
HDINRTEFEA LRKFVVGRGN FSVEDEILRL PLKACIENGV KLILEKLLVL HRVKADTILI 

       550        560        570        580        590        600 
KEALPFILCL GLDCHLKEKA PMPDTDNMVN ATAFLSGFKV LPRAPSRIGA RMGRPEKANL 

       610        620        630        640        650        660 
RKMSPAAQVL FPIGNAGGLT RNLVSASNYS VSMNGKIGEI EVEMGIRECP ACGKETYFWR 

       670        680        690        700        710        720 
CDCGEYTRPK LFCPRCKIEV RNSETCPKCG RKPTSVSNVK LDFRSIYKRA FENVGEREKM 

       730        740        750        760        770        780 
DLIKGVKRLM NGQMTPEPLE KGILRAKHDV YIFKDGTVRY DMSDIPLTHV RADEIGITAA 

       790        800        810        820        830        840 
KLRELGYVED IYGNPLERDD QIVCLKVQDL VISYDGGGYM LRTAQYVDDL LVKYYGVEPY 

       850        860        870        880        890        900 
YNAKTIQDLV GVLLIGLAPH TSAGVLGRLI GFTRASVGYA HPFFHASKRR NCDGDEDCVM 

       910        920        930        940        950        960 
LLMDGILNFS RAYLPEKRGG KMDAPLVLTT RIDPKEVDKE AHNIDLPARY PLEFYRATQE 

       970        980        990       1000       1010       1020 
IKNPTELEGI MDLVSSRLGT PEQYEHFMFT HDTSDIAAGP LNSSYKTLGS MIDKMEAQLS 

      1030       1040       1050       1060       1070       1080 
LANKIRAVDA PDVAERVLKS HFLPDLLGNL RSFSRQRMRC IKCGAKFRRP PLTGACPKCG 

      1090       1100       1110       1120       1130       1140 
GNVVLTVHEG AVRKYLEISK EIGKRYGVSS YTQQRIELLD KDICSLFENH RVKQLGISDF 


MSGSAR
Q46E19 in FASTA format

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