[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Andor; TISSUE=Leaf; Witt U., Gruneberg-Seiler M., Abel W.O.; "A full-length cDNA coding for mitochondrial malate dehydrogenase from Brassica napus L."; (er) Plant Gene Register PGR95-068.

Comments

CATALYTIC ACTIVITY: (S)-malate + NAD⁺ = oxaloacetate + NADH.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix.
SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X89451; CAA61621.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S57958; S57958.

3D structure databases

HSSP

P00346; 1MLD. [HSSP ENTRY / PDB]

ModBase

Q43744.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11540:SF1; MDH_euk_g_bac; 1.

Pfam

PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000102; Lac_mal_DH; 1.

TIGRFAMs

TIGR01772; MDH_euk_gproteo; 1.

PROSITE

PS00068; MDH; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 22`	`22`	`Mitochondrion (Potential).`
`CHAIN`	`23 341`	`319`	`Malate dehydrogenase, mitochondrial.`	`PRO_0000018623`
`NP_BIND`	`36 42`	`7`	`NAD (By similarity).`
`NP_BIND`	`145 147`	`3`	`NAD (By similarity).`
`BINDING`	`62 62`		`NAD (By similarity).`
`BINDING`	`109 109`		`Substrate (By similarity).`
`BINDING`	`115 115`		`Substrate (By similarity).`
`BINDING`	`122 122`		`NAD (By similarity).`
`BINDING`	`147 147`		`Substrate (By similarity).`
`BINDING`	`181 181`		`Substrate (By similarity).`
`BINDING`	`256 256`		`NAD (By similarity).`

Sequence information

Length: 341 AA [This is the length of the unprocessed precursor]

Molecular weight: 35711 Da [This is the MW of the unprocessed precursor]

CRC64: F27AC7D2680601C6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFRSALVRSS ASAKQSLLRR SFSSGSVPER KVAILGAAGG IGQPLALLMK LNPLVSSLSL 

        70         80         90        100        110        120 
YDIANTPGVA ADVGHINTRS QVVGYMGDDN LAKALEGADL VIIPAGVPRK PGMTRDDLFN 

       130        140        150        160        170        180 
INAGIVKNLW SAIAKYCPHA LVNMISNPVN STVPIAAEIF KKAGMYDEKK LFGVTTLDVV 

       190        200        210        220        230        240 
RVKTSYAGKA NVPVAEVNVP AIVGHAGVTI LPLFSQATPQ AILSGDALTV TTKRTQDGGT 

       250        260        270        280        290        300 
EVEEAKAGKG SATLSMAYAG ALFADACLKG LNGVPDVVEC SYVQSTITEL PFFASKVRLG 

       310        320        330        340 
KNGVEEVLDL GPLSDFEKEG LEALRPGIKS TIEKGVKFAN Q

Q43744 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools