NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Wando;
TISSUE=Leaf;
DOI=10.1104/pp.105.1.377; PubMed=8029359 [NCBI, ExPASy, EBI, Israel, Japan]
Williamson C.L., Slocum R.D.;
"Molecular cloning and characterization of the pyrB1 and pyrB2 genes encoding aspartate transcarbamoylase in pea (Pisum sativum L.).";
Plant Physiol. 105:377-384(1994).

Comments

CATALYTIC ACTIVITY: Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
ENZYME REGULATION: Allosterically regulated by UMP.
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6 .
SUBUNIT: Homotrimer (Potential).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the ATCase/OTCase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L15798; AAA62444.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T10181; T10181.

3D structure databases

HSSP

P00479; 1GQ3. [HSSP ENTRY / SWISS-3DIMAGE / PDB]

ModBase

Q43087.

Family and domain databases

InterPro

IPR006130; Asp/Orn_carbamoyltranf.
IPR006132; Asp/Orn_carbamoyltranf_P_bd.
IPR006131; Asp_carbamoyltransf_Asp/Orn_bd.
IPR002082; Aspartate_carbamoyltransf_euk.
Graphical view of domain structure.

Pfam

PF00185; OTCace; 1.
PF02729; OTCace_N; 1.
Pfam graphical view of domain structure.

PRINTS

PR00100; AOTCASE.
PR00101; ATCASE.

TIGRFAMs

TIGR00670; asp_carb_tr; 1.

PROSITE

PS00097; CARBAMOYLTRANSFERASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Allosteric enzyme; Chloroplast; Plastid; Pyrimidine biosynthesis; Transferase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 63`	`63`	`Chloroplast (Potential).`
`CHAIN`	`64 385`	`322`	`Aspartate carbamoyltransferase 2, chloroplastic.`	`PRO_0000020350`

Sequence information

Length: 385 AA [This is the length of the unprocessed precursor]

Molecular weight: 43745 Da [This is the MW of the unprocessed precursor]

CRC64: 1EC989FF0845EEB9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTASSSLFSC SMHMEVLTPK ISKWPKNFVS CHSKISYVET NYLKSTCYPI SRFFCINNLK 

        70         80         90        100        110        120 
KTRQRDGIHC FSEGQKFQLD DVVEAQQFDR DILNAIFEVA RDMEKIERNS PESQILKGYL 

       130        140        150        160        170        180 
MATLFYEPST RTRLSFESAM RRLGGEVLTT ENAREFSSAA KGETLEDTIR TVEGYSDLIV 

       190        200        210        220        230        240 
LRHFESGAAR RAATIAGIPI VNAGDGPGQH PSQALLDVYT IEREIGKLDG IKVGLVGDLA 

       250        260        270        280        290        300 
NGRTVRSLTY LLAKYKDVKI YFVSPEVVKM KDDIKDYLTS KGVDWEESSD LVEVASECDV 

       310        320        330        340        350        360 
VYQTRIQKER FGERLDLYEK ARGKFIVNQN ILNAMQRHAV IMHPLPRLDE ITVDVDADPR 

       370        380 
AAYFRQAKYG LYIRMALLKL LLVGW

Q43087 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools