ID   IAA12_ARATH             Reviewed;         239 AA.
AC   Q38830; Q8LA42;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   22-JUL-2008, entry version 61.
DE   RecName: Full=Auxin-responsive protein IAA12;
DE   AltName: Full=Indoleacetic acid-induced protein 12;
DE   AltName: Full=Protein BODENLOS;
GN   Name=IAA12; Synonyms=BDL; OrderedLocusNames=At1g04550;
GN   ORFNames=T1G11.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC   STRAIN=cv. Columbia;
RX   MEDLINE=95387393; PubMed=7658471; DOI=10.1006/jmbi.1995.0454;
RA   Abel S., Nguyen M.D., Theologis A.;
RT   "The PS-IAA4/5-like family of early auxin-inducible mRNAs in
RT   Arabidopsis thaliana.";
RL   J. Mol. Biol. 251:533-549(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   MUTANT BDL, AND INTERACTION WITH ARF5.
RX   PubMed=12101120; DOI=10.1101/gad.229402;
RA   Hamann T., Benkova E., Baeurle I., Kientz M., Juergens G.;
RT   "The Arabidopsis BODENLOS gene encodes an auxin response protein
RT   inhibiting MONOPTEROS-mediated embryo patterning.";
RL   Genes Dev. 16:1610-1615(2002).
RN   [6]
RP   GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX   MEDLINE=22031748; PubMed=12036262; DOI=10.1023/A:1015255030047;
RA   Liscum E., Reed J.W.;
RT   "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL   Plant Mol. Biol. 49:387-400(2002).
RN   [7]
RP   TRANSCRIPTIONAL REPRESSION DOMAIN.
RX   PubMed=14742873; DOI=10.1105/tpc.017384;
RA   Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT   "Aux/IAA proteins contain a potent transcriptional repression
RT   domain.";
RL   Plant Cell 16:533-543(2004).
CC   -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors
CC       that function as repressor of early auxin response genes at low
CC       auxin concentrations. Repression is thought to result from the
CC       interaction with auxin response factors (ARFs), proteins that bind
CC       to the auxin-responsive promoter element (AuxRE). Formation of
CC       heterodimers with ARF proteins may alter their ability to modulate
CC       early auxin response genes expression.
CC   -!- SUBUNIT: Homo and heterodimers. Interacts with the auxin response
CC       factor ARF5.
CC   -!- INTERACTION:
CC       Q9LPW7:AFB3; NbExp=1; IntAct=EBI-617608, EBI-617501;
CC       Q9ZR12:GRH1; NbExp=1; IntAct=EBI-617608, EBI-617479;
CC       Q570C0:TIR1; NbExp=1; IntAct=EBI-617608, EBI-307183;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=Q38830-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Preferentially expressed in stems, leaves and
CC       flowers.
CC   -!- INDUCTION: By auxin.
CC   -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC       domains I and II. Domain I includes a slightly degenerated ERF-
CC       associated amphiphilic repression (EAR) motif which seems to be
CC       involved in the activity of transcriptional repression. Domain II
CC       is required for the correct degradation of the protein through the
CC       SCF-mediated ubiquitin-proteasome pathway. Interactions between
CC       Aux/IAA proteins and auxin response factors (ARFs) occur through
CC       their C-terminal dimerization domains III and IV.
CC   -!- SIMILARITY: Belongs to the Aux/IAA family.
CC   -!- SIMILARITY: Contains 1 Aux/IAA-ARF domain.
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DR   EMBL; U18414; AAC49053.1; -; mRNA.
DR   EMBL; AC002376; AAB80631.1; -; Genomic_DNA.
DR   EMBL; AF332398; AAG48762.1; -; mRNA.
DR   EMBL; AY065240; AAL38716.1; -; mRNA.
DR   EMBL; AY096535; AAM20185.1; -; mRNA.
DR   EMBL; AY088042; AAM65588.1; -; mRNA.
DR   PIR; S58498; S58498.
DR   RefSeq; NP_171949.1; -.
DR   UniGene; At.10466; -.
DR   IntAct; Q38830; -.
DR   GeneID; 839495; -.
DR   GenomeReviews; CT485782_GR; AT1G04550.
DR   KEGG; ath:AT1G04550; -.
DR   NMPDR; fig|3702.1.peg.601; -.
DR   GeneFarm; 3159; 342.
DR   TAIR; At1g04550; -.
DR   ArrayExpress; Q38830; -.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR003311; AUX_IAA.
DR   InterPro; IPR011525; AuxIAA_ARF_dimer.
DR   Pfam; PF02309; AUX_IAA; 1.
DR   PROSITE; PS50962; IAA_ARF; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Auxin signaling pathway; Complete proteome;
KW   Nucleus; Repressor; Transcription; Transcription regulation.
FT   CHAIN         1    239       Auxin-responsive protein IAA12.
FT                                /FTId=PRO_0000112843.
FT   DOMAIN      125    218       Aux/IAA-ARF.
FT   MOTIF        20     24       EAR-like (transcriptional repression).
FT   MUTAGEN      74     74       P->S: In bdl; gain of function. Affects
FT                                auxin-related developmental processes.
FT   CONFLICT    119    119       P -> A (in Ref. 4; AAM65588).
SQ   SEQUENCE   239 AA;  26286 MW;  BBAADA3E98B222CA CRC64;
     MRGVSELEVG KSNLPAESEL ELGLGLSLGG GAWKERGRIL TAKDFPSVGS KRSAESSSHQ
     GASPPRSSQV VGWPPIGLHR MNSLVNNQAM KAARAEEGDG EKKVVKNDEL KDVSMKVNPK
     VQGLGFVKVN MDGVGIGRKV DMRAHSSYEN LAQTLEEMFF GMTGTTCREK VKPLRLLDGS
     SDFVLTYEDK EGDWMLVGDV PWRMFINSVK RLRIMGTSEA SGLAPRRQEQ KDRQRNNPV
//