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UniProtKB/Swiss-Prot entry Q38822


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IAA3_ARATH
Primary accession number Q38822
Secondary accession number O04741
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 67)
Name and origin of the protein
Protein name Auxin-responsive protein IAA3
Synonyms Indoleacetic acid-induced protein 3
Short hypocotyl
Suppressor of HY2
Gene name
Name: IAA3
Synonyms: SHY2
OrderedLocusNames: At1g04240
ORFNames: F19P19.32
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=cv. Columbia;
DOI=10.1006/jmbi.1995.0454; PubMed=7658471 [NCBI, ExPASy, EBI, Israel, Japan]
Abel S., Nguyen M.D., Theologis A.;
"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana.";
J. Mol. Biol. 251:533-549(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
MUTANTS SHY2-2 AND SHY2-3.
PubMed=9895319 [NCBI, ExPASy, EBI, Israel, Japan]
Tian Q., Reed J.W.;
"Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene.";
Development 126:711-721(1999).
[5]
PHOSPHORYLATION BY PHYTOCHROME A.
DOI=10.1104/pp.124.4.1728; PubMed=11115889 [NCBI, ExPASy, EBI, Israel, Japan]
Colon-Carmona A., Chen D.L., Yeh K.-C., Abel S.;
"Aux/IAA proteins are phosphorylated by phytochrome in vitro.";
Plant Physiol. 124:1728-1738(2000).
[6]
GENE FAMILY, NOMENCLATURE, AND FUNCTION.
DOI=10.1023/A:1015255030047; PubMed=12036262 [NCBI, ExPASy, EBI, Israel, Japan]
Liscum E., Reed J.W.;
"Genetics of Aux/IAA and ARF action in plant growth and development.";
Plant Mol. Biol. 49:387-400(2002).
[7]
TRANSCRIPTIONAL REPRESSION DOMAIN.
DOI=10.1105/tpc.017384; PubMed=14742873 [NCBI, ExPASy, EBI, Israel, Japan]
Tiwari S.B., Hagen G., Guilfoyle T.J.;
"Aux/IAA proteins contain a potent transcriptional repression domain.";
Plant Cell 16:533-543(2004).
Comments
  • FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that function as repressor of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.
  • SUBUNIT: Homo and heterodimers (By similarity).
  • INTERACTION:
    P14713:PHYB; NbExp=1; IntAct=EBI-307174, EBI-300727;
    Q570C0:TIR1; NbExp=1; IntAct=EBI-307174, EBI-307183;
  • SUBCELLULAR LOCATION: Nucleus (By similarity).
  • TISSUE SPECIFICITY: Highly expressed in stems and flowers.
  • INDUCTION: By auxin.
  • DOMAIN: The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.
  • PTM: Phosphorylated by phytochrome A in vitro.
  • SIMILARITY: Belongs to the Aux/IAA family.
  • SIMILARITY: Contains 1 Aux/IAA-ARF domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U18406; AAC49045.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC000104; AAB70452.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF332393; AAG48757.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY064007; AAL36363.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S58491; S58491.
RefSeq NP_171920.1; -.
UniGene At.20801
3D structure databases
ModBase Q38822.
Protein-protein interaction databases
IntAct Q38822; -.
Organism-specific databases
GeneFarm 3143; 342.
TAIR At1g04240; -.
Gene expression databases
ArrayExpress Q38822; -.
GermOnline AT1G04240; Arabidopsis thaliana.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR003311; AUX_IAA.
IPR011525; AuxIAA_ARF_dimer.
Graphical view of domain structure.
Pfam PF02309; AUX_IAA; 1.
Pfam graphical view of domain structure.
PROSITE PS50962; IAA_ARF; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q38822.
Proteomic databases
ProMEX Q38822; -.
Genome annotation databases
GeneID 839570; -.
GenomeReviews CT485782_GR; AT1G04240.
KEGG ath:AT1G04240; -.
NMPDR fig|3702.1.peg.570; -.
Other
LinkHub Q38822; -.
ProtoNet Q38822.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Auxin signaling pathway; Complete proteome; Nucleus; Phosphoprotein; Repressor; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   189  189     Auxin-responsive protein IAA3. PRO_0000112834
DOMAIN   93   180  88     Aux/IAA-ARF. 
MOTIF   12    16  5     EAR-like (transcriptional repression). 
COMPBIAS   33    36  4     Poly-Asn. 
MUTAGEN   67    67        G->E: In shy2-3; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis. 
MUTAGEN   69    69        P->S: In shy2-2; gain of function. Affects auxin-related developmental processes. Affects photomorphogenesis. 
Sequence information
Length: 189 AA [This is the length of the unprocessed precursor] Molecular weight: 21520 Da [This is the MW of the unprocessed precursor] CRC64: 8627A4173363EF27 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDEFVNLKET ELRLGLPGTD NVCEAKERVS CCNNNNKRVL STDTEKEIES SSRKTETSPP 

        70         80         90        100        110        120 
RKAQIVGWPP VRSYRKNNIQ SKKNESEHEG QGIYVKVSMD GAPYLRKIDL SCYKGYSELL 

       130        140        150        160        170        180 
KALEVMFKFS VGEYFERDGY KGSDFVPTYE DKDGDWMLIG DVPWEMFICT CKRLRIMKGS 


EAKGLGCGV 

Q38822 in FASTA format

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