[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Crossbred X Angus; TISSUE=Liver; NIH - Mammalian Gene Collection (MGC) project; Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC109798; AAI09799.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001068998.1; -.

UniGene

Bt.53371

3D structure databases

SMR

Q32L27; 168-329.

ModBase

Q32L27.

Family and domain databases

InterPro

IPR006575; RWD.
IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF05773; RWD; 1.
PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; FALSE_NEG.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q32L27.

Genome annotation databases

Ensembl

ENSBTAG00000004024; Bos taurus. [Contig view]

GeneID

511631; -.

KEGG

bta:511631; -.

Phylogenomic databases

HOVERGEN

Q32L27; -.

Other

ProtoNet

Q32L27.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Ligase; Nucleotide-binding; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 342`	`342`	`Ubiquitin-conjugating enzyme E2 Q2.`	`PRO_0000223878`
`COMPBIAS`	`41 55`	`15`	`Pro-rich.`
`COMPBIAS`	`102 145`	`44`	`Glu-rich.`
`ACT_SITE`	`271 271`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 342 AA [This is the length of the unprocessed precursor]

Molecular weight: 38717 Da [This is the MW of the unprocessed precursor]

CRC64: 5BB0CFBD4222BA51 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PPPAPPGSPH SPPPPLTLHC 

        70         80         90        100        110        120 
NITESYPSSS PIWFVDSDDP NLTSVLERLE DTKNNNSNGT TEEVTSEEEE EEEMAEDIED 

       130        140        150        160        170        180 
LDHYEMKEEE PISGKKSEDE GIEKENLAIL EKIRKTQRQD HLNGAVSGSV QASDRLMKEL 

       190        200        210        220        230        240 
RDIYRSQSYK TGIYSVELIN DSLYDWHVKL QKVDPDSPLH SDLQILKEKE GIEYILLNFS 

       250        260        270        280        290        300 
FKDNFPFDPP FVRVVLPVLS GGYVLGGGAL CMELLTKQGW SSAYSIESVI MQINATLVKG 

       310        320        330        340 
KARVQFGANK NQYNLARAQQ SYNSIVQIHE KNGWYTPPKE DG

Q32L27 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools