[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Crossbred X Angus; TISSUE=Liver; NIH - Mammalian Gene Collection (MGC) project; Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type IV membrane protein (By similarity).
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC111185; AAI11186.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001033132.1; -.

UniGene

Bt.32465

3D structure databases

SMR

Q2TA03; 11-171.

ModBase

Q2TA03.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; FALSE_NEG.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q2TA03.

Genome annotation databases

Ensembl

ENSBTAG00000015882; Bos taurus. [Contig view]

GeneID

506103; -.

KEGG

bta:506103; -.

Phylogenomic databases

HOVERGEN

Q2TA03; -.

Other

ProtoNet

Q2TA03.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Ligase; Membrane; Transmembrane; Ubl conjugation pathway.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 259`	`259`	`Ubiquitin-conjugating enzyme E2 J2.`	`PRO_0000245041`
`TOPO_DOM`	`1 226`	`226`	`Cytoplasmic (Potential).`
`TRANSMEM`	`227 247`	`21`	`Anchor for type IV membrane protein (Potential).`
`TOPO_DOM`	`248 259`	`12`	`Lumenal (Potential).`
`ACT_SITE`	`94 94`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 28848 Da [This is the MW of the unprocessed precursor]

CRC64: AFF8D77BD15BF8A4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSNSVKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 

        70         80         90        100        110        120 
YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 

       130        140        150        160        170        180 
FMVEKGPTLG SIETSDFTKR QLAAQSLVFN LKDKVFCELF PEVVEEIKQK QKAQDELSSR 

       190        200        210        220        230        240 
PQALPLPDVV PDGETHHGQH GLPLLNGHAP GAGPHLAGLQ QANRHHGLLG GALANLFVIV 

       250 
GFAAFAYTVK YVLRSIAQE

Q2TA03 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools