NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.188.2.642-658.2006; PubMed=16385054 [NCBI, ExPASy, EBI, Israel, Japan]
Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K.;
"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis.";
J. Bacteriol. 188:642-658(2006).

Comments

FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000102; ABC56671.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_447314.1; -.

3D structure databases

ModBase

Q2NHG2.

Enzyme and pathway databases

BioCyc

MSTA339860:MSP_0255-MON; -.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

TIGRFAMs

TIGR00354; polC; 1.

BLOCKS

Q2NHG2.

Genome annotation databases

GeneID

3855689; -.

GenomeReviews

CP000102_GR; Msp_0255.

KEGG

mst:Msp_0255; -.

NMPDR

fig|339860.6.peg.251; -.

Phylogenomic databases

HOGENOM

Q2NHG2; -.

Genome annotation databases

CMR

Q2NHG2; Msp_0255.

Other

ProtoNet

Q2NHG2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 1117`	`1117`		`DNA polymerase II large subunit.`	`PRO_0000294693`

Sequence information

Length: 1117 AA [This is the length of the unprocessed precursor]

Molecular weight: 124988 Da [This is the MW of the unprocessed precursor]

CRC64: DE1855748F768A1F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDYFGMLEEK TKELYAIARE ARKQGKDLEL EPEIPLAKDL AERVEGLVGP EGVAKRIKEL 

        70         80         90        100        110        120 
EKSMSREEVA FQIAKEIATK DDVEGQPNDY EVQEANADSA IRTALAILTE GVVAAPLEGI 

       130        140        150        160        170        180 
AKVKIKDNSD GTKCFGVYFA GPIRSAGGTA AALAVLLGDY IRMSQGLDRY KPTDDEIERY 

       190        200        210        220        230        240 
VEEVELYESE VTNLQYSPTP DEVRLAIRGI PVEVTGEQTD PVEVQNRDLP RVETNNLRGG 

       250        260        270        280        290        300 
ALLAVAEGVI QKSRKIVKYA KTLKIDGWDW LEYFTAPKST KEEEKKKEES SENKPKKKAK 

       310        320        330        340        350        360 
YMEDIIGGRP VMSYPGAKGG FRLRYGRTRD SGLASMAIHP ATMEIVEFLA IGTQMKIEKP 

       370        380        390        400        410        420 
GKGNCVVPCD SIEGPIVKLK NGDVIQVNDV SKAISIRRDV NEIIFLGDML VAFGEYLRGN 

       430        440        450        460        470        480 
IPLDVSAWCE EWWAQEIEAS EYFKETHDTF GIDFNENMEL NALLKLDIDA KKAFDIAKKT 

       490        500        510        520        530        540 
NTPLHPKFTF YYNDVTKEEL NDLHNYLYSL IDSPEDVFKS DMNRIPIDYH KRIIEVLGIP 

       550        560        570        580        590        600 
HHVNNKSLIM SNDNLYTLMS VLNKSLSPDE DMETIEAVNM ISPVSIKSKA PTYIGGRVGR 

       610        620        630        640        650        660 
PEKTKERLMK PAPHSLFPIG NYAGNIRNIV EAAKKGTIKV TLAKCKCTNP DCKVSSFKAL 

       670        680        690        700        710        720 
CPVCGSRTEL ESSAAKNIKL DKLLMDAFEN VNVRRLDEVK GVKGLISEDK YPEPLEKGIL 

       730        740        750        760        770        780 
RARNDVFTYR DGTIRHDSTD LPLTHFIPRE VGVPYEKILE MGYTEDIYGK PITNDNQIIE 

       790        800        810        820        830        840 
IKIQDIVVSE SCGDYLLKVS KFIDDLLIRY YHMEPFYNAE NRVDLVGHLI AGLAPHTSAG 

       850        860        870        880        890        900 
VLGRIVGFTK ASCCYAHPYF HSAKRRNCDS DEDAVMLLLD ALLNFGKTYL PSTRGGSMDA 

       910        920        930        940        950        960 
PLVLSIRIDP EEIDDESHNI DCMKRIPLEI YHKTEEGGVK PSDVNDLVDN VESRLGTDNQ 

       970        980        990       1000       1010       1020 
YHGLMYSHPT SSIHAGPKIC LYKTLQTMTD KVESQIALAE LLRAVDQKGV VEGVLNSHFL 

      1030       1040       1050       1060       1070       1080 
PDMAGNIRAF SRQKVRCTKC GAKYRRIPLS GECTCGNNLI LSISKGSVLK YLDISKDLSH 

      1090       1100       1110 
RYPINPYVVE RIEILETGIN SLFESDKSKQ SSLDAFF

Q2NHG2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools