[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. DOI=10.1186/1471-2164-6-166; PubMed=16305752 [NCBI, ExPASy, EBI, Israel, Japan] Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.; "Characterization of 954 bovine full-CDS cDNA sequences."; BMC Genomics 6:166-166(2005).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Hypothalamus; NIH - Mammalian Gene Collection (MGC) project; Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids (By similarity).
CATALYTIC ACTIVITY: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cytoplasmic vesicle (By similarity). Microsome (By similarity). Endoplasmic reticulum (By similarity).
SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
SEQUENCE CAUTION:
- Sequence=AAX46711.1; Type=Frameshift; Positions=705;

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BT021864; AAX46711.1; ALT_FRAME; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC112858; AAI12859.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001019719.1; -.

UniGene

Bt.37833

3D structure databases

ModBase

Q2KHW5.

Ontologies

GO:0031410;	Cellular component: cytoplasmic vesicle (inferred from electronic annotation from UniProtKB-KW).
GO:0005783;	Cellular component: endoplasmic reticulum (inferred from electronic annotation from UniProtKB-KW).
GO:0005792;	Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0004467;	Molecular function: long-chain-fatty-acid-CoA ligase activity (inferred from electronic annotation from EC).
GO:0006631;	Biological process: fatty acid metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.

Pfam

PF00501; AMP-binding; 1.
Pfam graphical view of domain structure.

PRINTS

PR00154; AMPBINDING.

PROSITE

PS00455; AMP_BINDING; 1.

ProtoNet

Q2KHW5.

Genome annotation databases

Ensembl

ENSBTAG00000006231; Bos taurus. [Contig view]

GeneID

515577; -.

KEGG

bta:515577; -.

Phylogenomic databases

HOVERGEN

Q2KHW5; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Microsome; Phosphoprotein.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 726`	`726`	`Long-chain-fatty-acid--CoA ligase ACSBG1.`	`PRO_0000315807`
`MOD_RES`	`118 118`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`121 121`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`122 122`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`641 641`		`Phosphotyrosine (By similarity).`

Sequence information

Length: 726 AA [This is the length of the unprocessed precursor]

Molecular weight: 80585 Da [This is the MW of the unprocessed precursor]

CRC64: 22EC0E08E143FB72 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPDSRAAPQE SLLDASLGTT QENVGTSSLT DGQTLSKEPL SHALKLSTPE KVKDAQLNPP 

        70         80         90        100        110        120 
EEALWTTRAD GRVRLRIDPI CSQTPRTVHQ MFSTTLDKYG DLSAMGFKRQ GTWEHISYTQ 

       130        140        150        160        170        180 
YYLLARKAAK GFLKLGLERA HSVAILAFNS PEWFFSAVGA VFGGGIITGI YTTSSPEACQ 

       190        200        210        220        230        240 
YIAYDCRANI IVVDTQKQLE KILKIWKHLP HLKAVVIYRE APPMRMPSVY TMEELMELGN 

       250        260        270        280        290        300 
EVPEEALDVI INAQKPNQCC ALVYTSGTTG NPKGVMLSQD NITWTARYGS QAGDIQPAEI 

       310        320        330        340        350        360 
QQEVVVSYLP LSHIAAQIYD LWTGIQWGAQ VCFAEPDALK GSLVNTLREV EPTSHMGVPR 

       370        380        390        400        410        420 
VWEKIMEQIQ EVAAQSGFIW RKMLLWAMSV TLEQNLTCPS SDLKPFTTRL ADYLVLAKVR 

       430        440        450        460        470        480 
QALGFAKCQK NFYGAAPMTA ETQHFFLGLN IRLYAGYGLS ETSGPHFMSS PYNYRLYSSG 

       490        500        510        520        530        540 
KVVPGCQVKL VNEDAEGIGE ICLWGRTIFM GYLNMEDKTC EAIDAEGWLH TGDTGRLDAD 

       550        560        570        580        590        600 
GFLYITGRLK ELIITAGGEN VPPVPIEEAV KTELPIIRNA MLIGDQRKFL SMLLTLKCTL 

       610        620        630        640        650        660 
DPDTFEPTDN LTEQAVEFCQ RVGSKATTVS EVVGKKDEAV YQAIEEGIQR VNMNAAARPY 

       670        680        690        700        710        720 
HIQKWAILEK DFSISGGELG PTMKLKRLAV LEKYKDVIDS FYQEQKSKQG SSLPGFSLRW 


QTGASS

Q2KHW5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools