ID   PYRC_ANADE              Reviewed;         433 AA.
AC   Q2IIB0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   02-SEP-2008, entry version 22.
DE   RecName: Full=Dihydroorotase;
DE            Short=DHOase;
DE            EC=3.5.2.3;
GN   Name=pyrC; OrderedLocusNames=Adeh_1619;
OS   Anaeromyxobacter dehalogenans (strain 2CP-C).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Anaeromyxobacter.
OX   NCBI_TaxID=290397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 3/6.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
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DR   EMBL; CP000251; ABC81392.1; -; Genomic_DNA.
DR   RefSeq; YP_464829.1; -.
DR   GeneID; 3888118; -.
DR   GenomeReviews; CP000251_GR; Adeh_1619.
DR   KEGG; ade:Adeh_1619; -.
DR   HOGENOM; Q2IIB0; -.
DR   BioCyc; ADEH290397:ADEH_1619-MON; -.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00220; -; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOmult.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    433       Dihydroorotase.
FT                                /FTId=PRO_0000325580.
FT   METAL        63     63       Zinc 1 (By similarity).
FT   METAL        65     65       Zinc 1 (By similarity).
FT   METAL       145    145       Zinc 1; via carbamate group (By
FT                                similarity).
FT   METAL       145    145       Zinc 2; via carbamate group (By
FT                                similarity).
FT   METAL       182    182       Zinc 2 (By similarity).
FT   METAL       235    235       Zinc 2 (By similarity).
FT   METAL       310    310       Zinc 1 (By similarity).
FT   MOD_RES     145    145       N6-carboxylysine (By similarity).
SQ   SEQUENCE   433 AA;  45437 MW;  36351670669BD114 CRC64;
     MSDVLFIEGG RVIDPAGGVD GVRTVVIRDG KVAEVAERVE RPRDARVLDA RNRWVTPGFV
     DLHVHLREPG QEYKETVATG ARAAVAGGFT AVCAMPNTKP VNDCAAVTEL VLARAAAAGL
     ARVYPVGAIS KGSGGEELAE YGELKASGCV ALSDDGRPVM SSALMRRALE YARAFGLPLT
     VHEEDLHLVG KGVMHEGAAA TRLGLKGIPS QAEDVMVLRD IALVELTGGR LHVAHVSTAG
     AVRAIREAKR RGLPVTGEVT PHHLALTDDD VAASGYSTDF KMNPPLRSAD DVRACREGLA
     DGTLDAIATD HAPHSAVEKD VEFDAAANGI VGLETAFSVC LGLVREGALT ERRLVEALTA
     GPARVFGLPA GTLARGAAAD VAVLDAAAEW TLDPARLQSK GRNTPWKGRR LAGRCTHTIV
     GGRIVHEEGK ADR
//