NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1016/S0140-6736(06)68231-7; PubMed=16517273 [NCBI, ExPASy, EBI, Israel, Japan]
Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.;
"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus.";
Lancet 367:731-739(2006).

Comments

FUNCTION: Member of the two-component regulatory system arlS/arlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to arlR (By similarity).
CATALYTIC ACTIVITY: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
PTM: Autophosphorylated (By similarity).
SIMILARITY: Contains 1 HAMP domain.
SIMILARITY: Contains 1 histidine kinase domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000255; ABD21101.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_494004.1; -.

3D structure databases

ModBase

Q2FH24.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0000155;	Molecular function: two-component sensor activity (inferred from electronic annotation from InterPro).
GO:0009405;	Biological process: pathogenesis (inferred from electronic annotation from UniProtKB-KW).
GO:0018106;	Biological process: peptidyl-histidine phosphorylation (inferred from electronic annotation from InterPro).
GO:0000160;	Biological process: two-component signal transduction system (phosphorelay) (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR003594; ATP_bd_ATPase.
IPR004358; Sig_transdc_His_kin-like_C.
IPR003661; Sig_transdc_His_kin_sub1_dim/P.
IPR005467; Sig_transdc_His_kinase_core.
IPR003660; Sig_transdc_His_kinase_HAMP.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.565.10; ATP_bd_ATPase; 1.

Pfam

PF00672; HAMP; 1.
PF02518; HATPase_c; 1.
PF00512; HisKA; 1.
Pfam graphical view of domain structure.

PRINTS

PR00344; BCTRLSENSOR.

SMART

SM00304; HAMP; 1.
SM00387; HATPase_c; 1.
SM00388; HisKA; 1.
SMART graphical view of domain structure.

PROSITE

PS50885; HAMP; 1.
PS50109; HIS_KIN; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q2FH24.

Genome annotation databases

GeneID

3913274; -.

GenomeReviews

CP000255_GR; SAUSA300_1307.

KEGG

saa:SAUSA300_1307; -.

Phylogenomic databases

HOGENOM

Q2FH24; -.

Genome annotation databases

CMR

Q2FH24; SAUSA300_1307.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Kinase; Membrane; Phosphoprotein; Transferase; Transmembrane; Two-component regulatory system; Virulence.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 451`	`451`	`Signal transduction histidine-protein kinase arlS.`	`PRO_0000293448`
`TRANSMEM`	`11 31`	`21`	`Potential.`
`TRANSMEM`	`156 176`	`21`	`Potential.`
`DOMAIN`	`178 231`	`54`	`HAMP.`
`DOMAIN`	`239 451`	`213`	`Histidine kinase.`
`MOD_RES`	`242 242`		`Phosphohistidine; by autocatalysis (By similarity).`

Sequence information

Length: 451 AA [This is the length of the unprocessed precursor]

Molecular weight: 52400 Da [This is the MW of the unprocessed precursor]

CRC64: 6308576067A22438 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTKRKLRNNW IIVTTMITFV TIFLFCLIII FFLKDTLHNS ELDDAERSSS DINNLFHSKP 

        70         80         90        100        110        120 
VKDISALDLN ASLGNFQEII IYDEHNNKLF ETSNDNTVRV EPGYEHRYFD RVIKKRYKGI 

       130        140        150        160        170        180 
EYLIIKEPIT TQDFKGYSLL IHSLENYDNI VKSLYIIALA FGVIATIITA TISYVFSTQI 

       190        200        210        220        230        240 
TKPLVSLSNK MIEIRRDGFQ NKLQLNTNYE EIDNLANTFN EMMSQIEESF NQQRQFVEDA 

       250        260        270        280        290        300 
SHELRTPLQI IQGHLNLIQR WGKKDPAVLE ESLNISIEEM NRIIKLVEEL LELTKGDVND 

       310        320        330        340        350        360 
ISSEAQTVHI NDEIRSRIHS LKQLHPDYQF DTDLTSKNLE IKMKPHQFEQ LFLIFIDNAI 

       370        380        390        400        410        420 
KYDVKNKKIK VKTRLKNKQK IIEITDHGIG IPEEDQDFIF DRFYRVDKSR SRSQGGNGLG 

       430        440        450 
LSIAQKIIQL NGGSIKIKSE INKGTTFKII F

Q2FH24 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools