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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Cartilage;
PubMed=9858406 [NCBI, ExPASy, EBI, Israel, Japan]
Richardson D.W.,
Dodge G.R.;
"Molecular characteristics of equine stromelysin and the tissue inhibitor of metalloproteinase 1.";
Am. J. Vet. Res. 59:1557-1562(1998).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Cartilage;
PubMed=9442239 [NCBI, ExPASy, EBI, Israel, Japan]
Balkman C.E.,
Nixon A.J.;
"Molecular cloning and cartilage gene expression of equine stromelysin 1 (matrix metalloproteinase 3).";
Am. J. Vet. Res. 59:30-36(1998).
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[3]
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3D-STRUCTURE MODELING.
Mallena S.C.,
Sharma J.A.R.P.;
"Theoretical model of horse stromelysin.";
Submitted (MAR-2002) to the PDB data bank.
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- FUNCTION: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
- CATALYTIC ACTIVITY: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.
- COFACTOR: Binds 4 calcium ions per subunit (By similarity).
- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (Probable).
- DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
- SIMILARITY: Belongs to the peptidase M10A family [view classification].
- SIMILARITY: Contains 4 hemopexin-like domains.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 477 AA [This is the length of the unprocessed precursor] |
Molecular weight: 54190 Da [This is the MW of the unprocessed precursor] |
CRC64: 361CE1427E09A272 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MKNLPILLLL CVAACSAYPL DRSARDEDSN MDLLQDYLEK YYDLGKEMRQ YVRRKDSGPI
70 80 90 100 110 120
VKKIQEMQKF LGLKVTGKLD SDTVEVMHKS RCGVPDVGHF TTFPGMPKWS KTHLTYRIVN
130 140 150 160 170 180
YTQDLPRDAV DSDVEKALKI WEEVTPLTFS RIYEGEADIM ITFAVREHGD FFPFDGPGKV
190 200 210 220 230 240
LAHAYPPGPG MNGDAHFDDD EHWTKDASGI NFLLVAAHEL GHSLGLYHST NTEALMYPLY
250 260 270 280 290 300
NTLKGPARVR LSQDDVTGIQ SLYGPPPASP DSPVEPSEPE PPAPGTLAMC DPALSFDAIS
310 320 330 340 350 360
TLRGEILFFK DRYFWRKTFR TLVPEFHPIS SFWPSLPSGI DAAYEVTSRD SVFIFKGNKF
370 380 390 400 410 420
WAIRGNEEQA GYPRGIHTLG FPPTVRKIDA AIFDKEKQKT YFFVEDKYWR FDEKRQSMEP
430 440 450 460 470
GYPKQIAEDF PGIDSKLDAA FESFGFFYFF SGSSQFEFDP NAKKVTHVLK SNSWFNC
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Q28397 in FASTA format |
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ExPASy Home page
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