NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
STRAIN=Bristol N2;
DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan]
The C. elegans sequencing consortium;
"Genome sequence of the nematode C. elegans: a platform for investigating biology.";
Science 282:2012-2018(1998).

Comments

FUNCTION: This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA.
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	a
Isoform ID	Q27474-1
Note: No experimental confirmation available.
This is the isoform sequence displayed in this entry.

Name	b
Isoform ID	Q27474-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_013778, VSP_013779.

SIMILARITY: Belongs to the ATP-dependent DNA ligase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z73970; CAA98242.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z73970; CAD44112.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

T19544; T19544.

NP_741625.2; -.

3D structure databases

ModBase

Q27474.

Organism-specific databases

WormBase

WBGene00002985; lig-1.

WormPep

C29A12.3a; CE37480. [WormPep / WorfDB]
C29A12.3b; CE37481. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

Q27474; -.

Ontologies

GO:0000910;	Biological process: cytokinesis (inferred from mutant phenotype from WormBase).
GO:0009792;	Biological process: embryonic development ending in birth or egg hatching (inferred from mutant phenotype from WormBase).
QuickGo view.

Family and domain databases

InterPro

IPR000977; DNA_ligase.
IPR012309; DNA_ligase_A_C.
IPR012310; DNA_ligase_A_M.
IPR012308; DNA_ligase_A_N.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF04679; DNA_ligase_A_C; 1.
PF01068; DNA_ligase_A_M; 1.
PF04675; DNA_ligase_A_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q27474.

Genome annotation databases

Ensembl

C29A12.3; Caenorhabditis elegans. [Contig view]

GeneID

179502; -.

KEGG

cel:C29A12.3; -.

NMPDR

fig|6239.3.peg.19533; -.

Other

ProtoNet

Q27474.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 773`	`773`	`DNA ligase.`	`PRO_0000059583`
`COMPBIAS`	`638 648`	`11`	`Poly-Glu.`
`COMPBIAS`	`660 762`	`103`	`Lys-rich.`
`ACT_SITE`	`289 289`		`N6-AMP-lysine intermediate (By similarity).`
`VAR_SEQ`	`208 210`		`VRG -> RMP (in isoform b).`	`VSP_013778`
`VAR_SEQ`	`211 773`		`Missing (in isoform b).`	`VSP_013779`

Sequence information

Length: 773 AA [This is the length of the unprocessed precursor]

Molecular weight: 86310 Da [This is the MW of the unprocessed precursor]

CRC64: 458B676F9FCE0BA0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFFSKAGKAA VEWAKGSKVP YKEFALTLEK IEELSGKKKV DELAQFFTKV LDFSPDDLTA 

        70         80         90        100        110        120 
CVYMSVNQLG PSYEGLELGV AENSLIKAVA KATGRTEGKI KEDLRAKGDL GTVAQQSRSN 

       130        140        150        160        170        180 
QKMLAVPKAL TVPTVFNKLT EIAKLSGTSA MNKKVDAISA LLIACQGIEA RFLVRMLAGK 

       190        200        210        220        230        240 
MRIGLGEQSV LSALGHAFTL SKITDQKVRG DKLDSLKDAN VKRVKTAYCE CPNYNRLIEV 

       250        260        270        280        290        300 
ALTEGVEALV EKCKLSPGIP LKPMLAHPTK GIDEIMRRFR NQTMTCEWKY DGERGQIHKR 

       310        320        330        340        350        360 
EDGQIFIYSR NQENNTTKYP DIIEKISSCI GDGVTSFIVD AEVVAIDEAG LILPFQVLST 

       370        380        390        400        410        420 
RKRKNATDDN GVKVVVFLFD LLYFNGEPLV RKPLRKRREL LRTNFKKIDG SFYFATSVDT 

       430        440        450        460        470        480 
NDTDEINSFF DEAVQNKCEG LMIKTLDTEA TYEISRRSHS WLKMKKDYVD GVGDTLDLVV 

       490        500        510        520        530        540 
MGAYSGVGKR TGVYGGYLLG CYNPTTEEYE SVCKIGTGFT DEDLAEQYKI LQDKKIDKSP 

       550        560        570        580        590        600 
SYYQFDHTLK PDDTFSPYLV FEVKCADITI SPRHKAASGL TDDGKGISLR FPRFLRIRDD 

       610        620        630        640        650        660 
KNSDDATSSE QVLEMYKNQE AFANQKIEKA DAVDEDDEFE EKEDEEEELN MTNVSEGSSK 

       670        680        690        700        710        720 
ENPVKEEIKK ETPKSVSPKK FEKKPPVKSS PVNKSPVKSS PIKKEAEKKK GPVASIFSSS 

       730        740        750        760        770 
TKKNEKDVKV ESPSPIRKKK LPSDSDESDE ETSTNKKQPA KKRSRVAIDS DSD

Q27474 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools