[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Tuebingen; The Danio rerio sequencing project at the Sanger Institute; Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the mRNA cap methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AL928820; CAK03687.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001038465.1; -.

UniGene

Dr.68392

3D structure databases

ModBase

Q1MTD3.

Organism-specific databases

ZFIN

ZDB-GENE-041008-25; si:ch211-105d11.3.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from sequence or structural similarity from UniProtKB).
GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0003723;	Molecular function: RNA binding (inferred from sequence or structural similarity from UniProtKB).
GO:0006370;	Biological process: mRNA capping (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR016899; mRNA_G-N7_MeTrfase.
IPR004971; Pox_MCEL.
Graphical view of domain structure.

Pfam

PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF028762; ABD1; 1.

BLOCKS

Q1MTD3.

Genome annotation databases

Ensembl

ENSDARG00000061926; Danio rerio. [Contig view]
ENSDARG00000070553; Danio rerio. [Contig view]

GeneID

562801; -.

KEGG

dre:562801; -.

NMPDR

fig|7955.3.peg.10345; -.

Phylogenomic databases

HOVERGEN

Q1MTD3; -.

Other

ProtoNet

Q1MTD3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; mRNA capping; mRNA processing; Nucleus; RNA-binding; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 400`	`400`	`mRNA cap guanine-N7 methyltransferase.`	`PRO_0000248325`
`REGION`	`100 104`	`5`	`mRNA cap-binding (By similarity).`
`REGION`	`185 186`	`2`	`S-adenosyl-L-methionine-binding (By similarity).`
`REGION`	`208 210`	`3`	`S-adenosyl-L-methionine-binding (By similarity).`
`BINDING`	`104 104`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`128 128`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`
`BINDING`	`137 137`		`mRNA cap (By similarity).`
`BINDING`	`150 150`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`162 162`		`mRNA cap (By similarity).`
`BINDING`	`391 391`		`mRNA cap (By similarity).`

Sequence information

Length: 400 AA [This is the length of the unprocessed precursor]

Molecular weight: 45961 Da [This is the MW of the unprocessed precursor]

CRC64: 271B592DBCF1663B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMAQNSRLFE MDSPYADAKV DKDSIGSSFD SSQTSTSSSS SSVKRRRDGD EEDDDHSPSK 

        70         80         90        100        110        120 
KLVTEDSLHS QKVATHYNKI KECGLAERNK SRIVYMRNFN NWLKSVLIAE ILDKVRQKRR 

       130        140        150        160        170        180 
EVTVLDLGCG KGGDLLKWKK GRIDKLVCAD IAAVSIEQCQ QRYNDVRRRG HPNDRTFSAE 

       190        200        210        220        230        240 
FITADCSREL LSEKLQDPEL QFDVCSCQFV YHYSFESESQ ADTMLRNACE RLRPGGFFIG 

       250        260        270        280        290        300 
TTPDAYELVK RLEESDSNSF GNEVFSVTFQ KKGEYPLFGC QYDFSLEGVV NVPEFLVYFP 

       310        320        330        340        350        360 
LFVEMAKKYN MRLVYKKTFK EFFEEKVKDG KNKDLMQWMQ ALEQYPPDER GQLSSSGPGE 

       370        380        390        400 
YDHAKRKAAD PAVRRPLGTL SKSEWEATSI YLVYVFEKMS

Q1MTD3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools