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UniProtKB/Swiss-Prot entry Q13094


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LCP2_HUMAN
Primary accession number Q13094
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 82)
Name and origin of the protein
Protein name Lymphocyte cytosolic protein 2
Synonyms SH2 domain-containing leukocyte protein of 76 kDa
SLP-76 tyrosine phosphoprotein
SLP76
Gene name
Name: LCP2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Leukemia;
DOI=10.1074/jbc.270.13.7029; PubMed=7706237 [NCBI, ExPASy, EBI, Israel, Japan]
Jackman J.K., Motto D.G., Sun Q., Tanemoto M., Turck C.W., Peltz G.A., Koretzky G.A., Findell P.R.;
"Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells.";
J. Biol. Chem. 270:7029-7032(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
INTERACTION WITH PRAM1.
DOI=10.1074/jbc.M011683200; PubMed=11301322 [NCBI, ExPASy, EBI, Israel, Japan]
Moog-Lutz C., Peterson E.J., Lutz P.G., Eliason S., Cave-Riant F., Singer A., Di Gioia Y., Dmovski S., Kamens J., Cayre Y.E., Koretzky G.;
"PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells.";
J. Biol. Chem. 276:22375-22381(2001).
[4]
INTERACTION WITH SHB.
PubMed=12084069 [NCBI, ExPASy, EBI, Israel, Japan]
Lindholm C.K., Henriksson M.L., Hallberg B., Welsh M.;
"Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells.";
Eur. J. Biochem. 269:3279-3288(2002).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, AND MASS SPECTROMETRY.
TISSUE=T-cell;
DOI=10.1021/ac035352d; PubMed=15144186 [NCBI, ExPASy, EBI, Israel, Japan]
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.;
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.";
Anal. Chem. 76:2763-2772(2004).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-173 AND SER-207, AND MASS SPECTROMETRY.
TISSUE=T-cell;
DOI=10.1038/nmeth776; PubMed=16094384 [NCBI, ExPASy, EBI, Israel, Japan]
Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R.;
"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry.";
Nat. Methods 2:591-598(2005).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, AND MASS SPECTROMETRY.
TISSUE=Platelet;
DOI=10.1021/pr0704130; PubMed=18088087 [NCBI, ExPASy, EBI, Israel, Japan]
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.;
"Phosphoproteome of resting human platelets.";
J. Proteome Res. 7:526-534(2008).
Comments
  • FUNCTION: Involved in T-cell antigen receptor mediated signaling.
  • SUBUNIT: Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.
  • INTERACTION:
    P08631:HCK; NbExp=1; IntAct=EBI-346946, EBI-346340;
  • SUBCELLULAR LOCATION: Cytoplasm (Probable).
  • TISSUE SPECIFICITY: Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.
  • DOMAIN: The SH2 domain mediates interaction with SHB.
  • PTM: Phosphorylated after T-cell receptor activation by ZAP-70.
  • SIMILARITY: Contains 1 SH2 domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U20158; AAC50135.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC016618; AAH16618.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A56110; A56110.
RefSeq NP_005556.1; -.
UniGene Hs.304475
3D structure databases
PDB
1H3H; NMR; -; B=232-241.[ExPASy / RCSB / EBI]
1YWO; X-ray; 1.81 A; P=185-194.[ExPASy / RCSB / EBI]
2EAP; NMR; -; A=1-83.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1H3H; -.
1YWO; -.
2EAP; -.
ModBase Q13094.
Protein-protein interaction databases
IntAct Q13094; -.
PTM databases
PhosphoSite Q13094; -.
Organism-specific databases
H-InvDB HIX0005402; -.
HIX0005403; -.
HGNC HGNC:6529; LCP2.
GenAtlas LCP2.
HPA CAB004574; -.
MIM 601603; gene. [NCBI / EBI]
PharmGKB PA30313; -.
GeneCards Q13094.
Gene expression databases
ArrayExpress Q13094; -.
CleanEx HS_LCP2; -.
GermOnline ENSG00000043462; Homo sapiens.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from experiment from Reactome).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006955; Biological process: immune response (traceable author statement from ProtInc).
GO:0007169; Biological process: transmembrane receptor protein tyrosine kinase signaling pathway (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR001660; SAM.
IPR011510; SAM_2.
IPR000980; SH2.
Graphical view of domain structure.
Gene3D G3DSA:3.30.505.10; SH2; 1.
Pfam PF07647; SAM_2; 1.
PF00017; SH2; 1.
Pfam graphical view of domain structure.
PRINTS PR00401; SH2DOMAIN.
ProDom PD000093; SH2; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00454; SAM; 1.
SM00252; SH2; 1.
SMART graphical view of domain structure.
PROSITE PS50001; SH2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q13094.
Genome annotation databases
Ensembl ENSG00000043462; Homo sapiens. [Contig view]
GeneID 3937; -.
KEGG hsa:3937; -.
Phylogenomic databases
HOGENOM Q13094; -.
HOVERGEN Q13094; -.
Other
SOURCE LCP2; Homo sapiens.
ProtoNet Q13094.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cytoplasm; Direct protein sequencing; Phosphoprotein; SH2 domain.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   533  533     Lymphocyte cytosolic protein 2. PRO_0000084368
DOMAIN   422   530  109     SH2. 
COMPBIAS   133   136  4     Poly-Glu. 
COMPBIAS   198   201  4     Poly-Pro. 
MOD_RES   23    23        Phosphotyrosine (By similarity). 
MOD_RES   173   173        Phosphotyrosine. 
MOD_RES   207   207        Phosphoserine. 
STRAND   1     4  4      
HELIX   9    12  4      
TURN   17    19  3      
HELIX   20    26  7      
HELIX   30    38  9      
HELIX   43    47  5      
HELIX   51    54  4      
TURN   59    61  3      
HELIX   62    73  12      
HELIX   237   239  3      
Sequence information
Length: 533 AA [This is the length of the unprocessed precursor] Molecular weight: 60188 Da [This is the MW of the unprocessed precursor] CRC64: C5D22F31D36200C8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MALRNVPFRS EVLGWDPDSL ADYFKKLNYK DCEKAVKKYH IDGARFLNLT ENDIQKFPKL 

        70         80         90        100        110        120 
RVPILSKLSQ EINKNEERRS IFTRKPQVPR FPEETESHEE DNGGWSSFEE DDYESPNDDQ 

       130        140        150        160        170        180 
DGEDDGDYES PNEEEEAPVE DDADYEPPPS NDEEALQNSI LPAKPFPNSN SMYIDRPPSG 

       190        200        210        220        230        240 
KTPQQPPVPP QRPMAALPPP PAGRNHSPLP PPQTNHEEPS RSRNHKTAKL PAPSIDRSTK 

       250        260        270        280        290        300 
PPLDRSLAPF DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL 

       310        320        330        340        350        360 
PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS PMNPLPSSHM 

       370        380        390        400        410        420 
PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL PLPNKPRPPS PAEEENSLNE 

       430        440        450        460        470        480 
EWYVSYITRP EAEAALRKIN QDGTFLVRDS SKKTTTNPYV LMVLYKDKVY NIQIRYQKES 

       490        500        510        520        530 
QVYLLGTGLR GKEDFLSVSD IIDYFRKMPL LLIDGKNRGS RYQCTLTHAA GYP 

Q13094 in FASTA format

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