FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000300; ABE53274.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_567024.1; -.

3D structure databases

ModBase

Q12TF2.

Enzyme and pathway databases

BioCyc

MBUR259564:MBUR_2423-MON; -.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

TIGRFAMs

TIGR00354; polC; 1.

BLOCKS

Q12TF2.

Genome annotation databases

GeneID

3999013; -.

GenomeReviews

CP000300_GR; Mbur_2423.

KEGG

mbu:Mbur_2423; -.

NMPDR

fig|259564.8.peg.2327; -.

Phylogenomic databases

HOGENOM

Q12TF2; -.

Genome annotation databases

CMR

Q12TF2; Mbur_2423.

Other

ProtoNet

Q12TF2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 1145`	`1145`		`DNA polymerase II large subunit.`	`PRO_0000294685`

Sequence information

Length: 1145 AA [This is the length of the unprocessed precursor]

Molecular weight: 127582 Da [This is the MW of the unprocessed precursor]

CRC64: 2E4E696ABF9D18E4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEIVASDEM NEYFNTLEGT LKKEIDIVND ARSRGKDPKP HVEIPLAKDL ADRVENLIGV 

        70         80         90        100        110        120 
KGVAELIRKL EETMSREEAA LALGREVAQG KVGEFDSKSE AIEAAIRVSV AMLTEGVVAA 

       130        140        150        160        170        180 
PIEGIDRASI GKNDDGSEYV SIFYAGPIRS AGGTAQALSV LVGDYVRRGV GIDRYKPRKE 

       190        200        210        220        230        240 
EVERYIEEIM LYKRVASLQY TPSEEEIRLI VENCPICIDG EPTEAEEVEG HRNLERIDTN 

       250        260        270        280        290        300 
RVRGGMALVL AEGLALKAPK IQKHVKNLKI DGWEWLEQLI SGVKSSSESD EDEETDGKPK 

       310        320        330        340        350        360 
IKPKDKYMRD LIAGRPVFSH PSRPGGFRLR YGRSRNTSFA AAGISPAGMI VMDDFIAPGT 

       370        380        390        400        410        420 
QLKVERPGKA AGMAPVDSIE GPTVRLNNGD VIRIDTIDEA YALRSEVEEI IDIGEILINY 

       430        440        450        460        470        480 
GDFLENNHPL APSPYCFEWW IQEYRKAAPD TETNEAELKE PTQEVALDLC KELNIPLHPK 

       490        500        510        520        530        540 
FTYLWHDIDN SQYTALADLI SKDGLLEADG SFLKLPLQRT IDTGMKKVLE DLLVQHKIQG 

       550        560        570        580        590        600 
QALTIEEPLP LIHSLGLDEE LSKGWDSLGH EELLENINEI AGFVVRPRAP TRIGARMGRP 

       610        620        630        640        650        660 
EKSDKRKMTP APHALFPIAE AGGNTRSLEK AANFKVNTNS KAGTIPVEIG NRICPACGVE 

       670        680        690        700        710        720 
GFEFRCECGE YTLPKLFCPR CGISVNKEKC PKCNSKTTCT SMRKIDFKSI YQKAFESIGE 

       730        740        750        760        770        780 
RDHLDSFKGV KKMMSKHMTP EPLEKGILRA KHGLFTFKDG TVRYDMSDIP LTHIRPAEIG 

       790        800        810        820        830        840 
VSCERMIELG YLKDIYGKPL IDSEQVLCLK VQDLVISYDA ADYILRITQY IDDLLVKYYK 

       850        860        870        880        890        900 
VAPYYNAKHI DDIVGVLLMG LAPHTSAGVL GRLIGFTTAS VGYAHPYFHA AKRRNCDGDE 

       910        920        930        940        950        960 
DCVMLLMDGL LNFSRDYLPD KRGGQMDAPL VLTTRLDPSE VDKEAHNIDM CASYPLEFYE 

       970        980        990       1000       1010       1020 
ATQNIANPKD FEGTMDLVSG RLGTTLQYEE FMFTHDTSNI AAGPLKSAYK TLGTMVEKMD 

      1030       1040       1050       1060       1070       1080 
AQLELAKKIR AVDAPDVAER VLTSHFLPDM FGNLRAFSRQ RTRCVKCAAK FRRPPLTGSC 

      1090       1100       1110       1120       1130       1140 
PKCGGRVILT VHEGAVKKYL QVSIKIAEEY NVSSYTKQRI ELIGYDMKSL FENDKSKQMG 


LSDFM

Q12TF2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools