[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
PubMed=8290273 [NCBI, ExPASy, EBI, Israel, Japan]
Toksoz D.,
Williams D.A.;
"Novel human oncogene lbc detected by transfection with distinct homology regions to signal transduction products.";
Oncogene 9:621-628(1994).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ESR1; THRA AND PPARA, AND VARIANT SER-2457.
TISSUE=Testis;
DOI=10.1038/sj.onc.1201783; PubMed=9627117 [NCBI, ExPASy, EBI, Israel, Japan]
Rubino D.,
Driggers P.,
Arbit D.,
Kemp L.,
Miller B.,
Coso O.,
Pagliai K.,
Gray K.,
Gutkind S.,
Segars J.;
"Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action.";
Oncogene 16:2513-2526(1998).
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[3]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 7), ALTERNATIVE SPLICING (ISOFORM 6), FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND TISSUE SPECIFICITY.
TISSUE=Skeletal muscle;
PubMed=9891067 [NCBI, ExPASy, EBI, Israel, Japan]
Sterpetti P.,
Hack A.A.,
Bashar M.P.,
Park B.,
Cheng S.-D.,
Knoll J.H.M.,
Urano T.,
Feig L.A.,
Toksoz D.;
"Activation of the Lbc Rho exchange factor proto-oncogene by truncation of an extended C terminus that regulates transformation and targeting.";
Mol. Cell. Biol. 19:1334-1345(1999).
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[4]
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ERRATUM.
Sterpetti P.,
Hack A.A.,
Bashar M.P.,
Park B.,
Cheng S.D.,
Knoll J.H.,
Urano T.,
Feig L.A.,
Toksoz D.;
Mol. Cell. Biol. 19:3930-3930(1999).
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[5]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH RHOA, AND VARIANTS CYS-574 AND SER-2457.
DOI=10.1016/S0014-5793(01)02995-7; PubMed=11696353 [NCBI, ExPASy, EBI, Israel, Japan]
Klussmann E.,
Edemir B.,
Pepperle B.,
Tamma G.,
Henn V.,
Klauschenz E.,
Hundsrucker C.,
Maric K.,
Rosenthal W.;
"Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling.";
FEBS Lett. 507:264-268(2001).
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[6]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RHOA; RHOB AND RHOC, MUTAGENESIS OF ALA-1251; ILE-1260 AND TYR-2153, AND VARIANTS VAL-624; MET-897 AND SER-2457.
DOI=10.1074/jbc.M106629200; PubMed=11546812 [NCBI, ExPASy, EBI, Israel, Japan]
Diviani D.,
Soderling J.,
Scott J.D.;
"AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated stress fiber formation.";
J. Biol. Chem. 276:44247-44257(2001).
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[7]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANTS THR-452; ARG-494; CYS-574; LYS-689; ALA-845; MET-897; ALA-1062; ASN-1086 AND THR-1216.
TISSUE=Lung;
Miyamoto M.,
Ono Y.;
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
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[8]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[9]
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NUCLEOTIDE SEQUENCE [MRNA] OF 754-1768, PKA AND RII BINDING, AND MUTAGENESIS OF ALA-1265.
TISSUE=Thyroid;
PubMed=1618839 [NCBI, ExPASy, EBI, Israel, Japan]
Carr D.W.,
Hausken Z.E.,
Fraser I.D.C.,
Stofko-Hahn R.E.,
Scott J.D.;
"Association of the type II cAMP-dependent protein kinase with a human thyroid RII-anchoring protein. Cloning and characterization of the RII-binding domain.";
J. Biol. Chem. 267:13376-13382(1992).
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[10]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1486-2813 (ISOFORM 4), AND VARIANT SER-2457.
TISSUE=Brain;
Totoki Y.,
Toyoda A.,
Takeda T.,
Sakaki Y.,
Tanaka A.,
Yokoyama S.,
Ohara O.,
Nagase T.,
Kikuno R.F.;
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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[11]
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FUNCTION, AND INTERACTION WITH ESR2.
DOI=10.1074/jbc.M106927200; PubMed=11579095 [NCBI, ExPASy, EBI, Israel, Japan]
Driggers P.H.,
Segars J.H.,
Rubino D.M.;
"The proto-oncoprotein Brx activates estrogen receptor beta by a p38 mitogen-activated protein kinase pathway.";
J. Biol. Chem. 276:46792-46797(2001).
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[12]
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INTERACTION WITH NME2.
DOI=10.1016/j.bbrc.2004.06.067; PubMed=15249197 [NCBI, ExPASy, EBI, Israel, Japan]
Iwashita S.,
Fujii M.,
Mukai H.,
Ono Y.,
Miyamoto M.;
"Lbc proto-oncogene product binds to and could be negatively regulated by metastasis suppressor nm23-H2.";
Biochem. Biophys. Res. Commun. 320:1063-1068(2004).
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[13]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1929 AND SER-1932, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V.,
Blagoev B.,
Gnad F.,
Macek B.,
Kumar C.,
Mortensen P.,
Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
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[14]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-983 AND SER-2728, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1038/nbt1240; PubMed=16964243 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A.,
Villen J.,
Gerber S.A.,
Rush J.,
Gygi S.P.;
"A probability-based approach for high-throughput protein phosphorylation analysis and site localization.";
Nat. Biotechnol. 24:1285-1292(2006).
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[15]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-983, AND MASS SPECTROMETRY.
DOI=10.2116/analsci.24.161; PubMed=18187866 [NCBI, ExPASy, EBI, Israel, Japan]
Imami K.,
Sugiyama N.,
Kyono Y.,
Tomita M.,
Ishihama Y.;
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Anal. Sci. 24:161-166(2008).
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[16]
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STRUCTURE BY NMR OF 493-516.
DOI=10.1093/emboj/20.7.1651; PubMed=11285229 [NCBI, ExPASy, EBI, Israel, Japan]
Newlon M.G.,
Roy M.,
Morikis D.,
Carr D.W.,
Westphal R.,
Scott J.D.,
Jennings P.A.;
"A novel mechanism of PKA anchoring revealed by solution structures of anchoring complexes.";
EMBO J. 20:1651-1662(2001).
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- FUNCTION: Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
- SUBUNIT: Binds cAMP-dependent protein kinase (PKA) and to the RII-alpha regulatory subunit of PKA. Interacts with ESR1, ESR2, THRA, PPARA, RHOA and NME2.
- INTERACTION:
Q16611:BAK1; NbExp=1; IntAct=EBI-1373806, EBI-519866;
P21980:TGM2; NbExp=3; IntAct=EBI-1373806, EBI-727668;
P31946:YWHAB; NbExp=1; IntAct=EBI-1373806, EBI-359815;
- SUBCELLULAR LOCATION: Isoform 3: Cytoplasm. Nucleus.
- SUBCELLULAR LOCATION: Isoform 2: Cytoplasm.
- SUBCELLULAR LOCATION: Isoform 6: Cytoplasm.
- SUBCELLULAR LOCATION: Isoform 7: Membrane.
- ALTERNATIVE PRODUCTS:
7 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Isoform ID | Q12802-2 |
| Features which should be applied to build the isoform sequence: VSP_023490. |
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- TISSUE SPECIFICITY: Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
- DOMAIN: Both the DH and PH domains are required for transforming activity.
- SIMILARITY: Contains 1 DH (DBL-homology) domain.
- SIMILARITY: Contains 1 PH domain.
- SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger.
- SEQUENCE CAUTION:
- Sequence=AAD21311.1; Type=Frameshift; Positions=2614, 2647;
- Sequence=AAL40923.1; Type=Frameshift; Positions=2614, 2647;
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