EC 2.4.1.69
GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
Alpha(1,2)FT 2
Fucosyltransferase 2
Secretor blood group alpha-2-fucosyltransferase
Secretor factor
SE2
Se

Gene name

	Name:	FUT2
	Synonyms:	SEC2

From

Homo sapiens (Human)

[TaxID: 9606]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG). DOI=10.1074/jbc.270.9.4640; PubMed=7876235 [NCBI, ExPASy, EBI, Israel, Japan] Kelly R.J., Rouquier S., Giorgi D., Lennon G.G., Lowe J.B.; "Sequence and expression of a candidate for the human Secretor blood group alpha(1,2)fucosyltransferase gene (FUT2). Homozygosity for an enzyme-inactivating nonsense mutation commonly correlates with the non-secretor phenotype."; J. Biol. Chem. 270:4640-4649(1995).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), AND VARIANT PHE-140. DOI=10.1074/jbc.271.16.9830; PubMed=8621666 [NCBI, ExPASy, EBI, Israel, Japan] Kudo T., Iwasaki H., Nishihara S., Shinya N., Ando T., Narimatsu I., Narimatsu H.; "Molecular genetic analysis of the human Lewis histo-blood group system. II. Secretor gene inactivation by a novel single missense mutation A385T in Japanese nonsecretor individuals."; J. Biol. Chem. 271:9830-9837(1996).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), AND VARIANT PHE-140. PubMed=9219535 [NCBI, ExPASy, EBI, Israel, Japan] Koda Y., Soejima M., Wang B., Kimura H.; "Structure and expression of the gene encoding secretor-type galactoside 2-alpha-L-fucosyltransferase (FUT2)."; Eur. J. Biochem. 246:750-755(1997).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS VAL-25; CYS-138; ASN-172 AND SER-258. SeattleSNPs program for genomic applications; Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
[5]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-343. PubMed=8755920 [NCBI, ExPASy, EBI, Israel, Japan] Koda Y., Soejima M., Liu Y., Kimura H.; "Molecular basis for secretor type alpha(1,2)-fucosyltransferase gene deficiency in a Japanese population: a fusion gene generated by unequal crossover responsible for the enzyme deficiency."; Am. J. Hum. Genet. 59:343-350(1996).
[6]	VARIANTS VAL-25; CYS-138 AND ASN-172. DOI=10.1007/s004390050808; PubMed=9760207 [NCBI, ExPASy, EBI, Israel, Japan] Liu Y., Koda Y., Soejima M., Pang H., Schlaphoff T., du Toit E.D., Kimura H.; "Extensive polymorphism of the FUT2 gene in an African (Xhosa) population of South Africa."; Hum. Genet. 103:204-210(1998).

Comments

FUNCTION: Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values.
CATALYTIC ACTIVITY: GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
PATHWAY: Protein modification; protein glycosylation .
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative initiation.

Name Long

Isoform ID Q10981-1

This is the isoform sequence displayed in this entry.

Name Short

Isoform ID Q10981-2

Features which should be applied to build the isoform sequence: VSP_018736.
TISSUE SPECIFICITY: Small intestine, colon and lung.
POLYMORPHISM: Three alleles have been identified in Japanese: Se1, Se2, and Sej.
MISCELLANEOUS: There are two genes (FUT1 and FUT2) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner with expression restricted to cells of mesodermal or endodermal origin respectively.
SIMILARITY: Belongs to the glycosyltransferase 11 family.
WEB RESOURCE: Name=BGMUT; Note=Blood group antigen gene mutation database"; URL="http://www.ncbi.nlm.nih.gov/projects/mhc/xslcgi.fcgi?cmd=bgmut/systems_info&system=hh";.
WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/";.
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/fut2/";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U17894; AAC24453.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D89327; BAA13944.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D87942; BAA21684.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY937240; AAX14047.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D82933; BAA11638.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A56098; A56098.

RefSeq

NP_000502.4; -.
NP_001091107.1; -.

UniGene

Hs.579928

3D structure databases

ModBase

Q10981.

Organism-specific databases

HIX0023049; -.

HGNC:4013; FUT2.

HPA014402; -.

182100; gene+phenotype. [NCBI / EBI]

PharmGKB

PA28429; -.

GeneCards

Q10981.

Gene expression databases

ArrayExpress

Q10981; -.

CleanEx

HS_FUT2; -.

GermOnline

ENSG00000176920; Homo sapiens.

Ontologies

GO:0030173;	Cellular component: integral to Golgi membrane (non-traceable author statement from UniProtKB).
GO:0008107;	Molecular function: galactoside 2-alpha-L-fucosyltransferase activity (traceable author statement from UniProtKB).
GO:0042355;	Biological process: L-fucose catabolic process (non-traceable author statement from UniProtKB).
GO:0006486;	Biological process: protein amino acid glycosylation (traceable author statement from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR002516; Glyco_trans_11.
Graphical view of domain structure.

PANTHER

PTHR11927; Glyco_trans_11; 1.

Pfam

PF01531; Glyco_transf_11; 1.
Pfam graphical view of domain structure.

BLOCKS

Q10981.

Genome annotation databases

Ensembl

ENSG00000176920; Homo sapiens. [Contig view]

GeneID

2524; -.

KEGG

hsa:2524; -.

Phylogenomic databases

HOGENOM

Q10981; -.

HOVERGEN

Q10981; -.

Other

FUT2; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative initiation; Blood group antigen; Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane; Polymorphism; Signal-anchor; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 343`	`343`	`Galactoside 2-alpha-L-fucosyltransferase 2.`	`PRO_0000012140`
`TOPO_DOM`	`1 14`	`14`	`Cytoplasmic (Potential).`
`TRANSMEM`	`15 28`	`14`	`Signal-anchor for type II membrane protein (Potential).`
`TOPO_DOM`	`29 343`	`315`	`Lumenal (Potential).`
`CARBOHYD`	`188 188`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`282 282`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`308 308`		`N-linked (GlcNAc...) (Potential).`
`VAR_SEQ`	`1 11`		`Missing (in isoform Short).`	`VSP_018736`
`VARIANT`	`25 25`	`1`	`I -> V (polymorphism found in Xhosa population; dbSNP:rs1800021 [NCBI]).`	`VAR_003422`
`VARIANT`	`138 138`	`1`	`R -> C (polymorphism found in Xhosa population; dbSNP:rs1800022 [NCBI]).`	`VAR_003423`
`VARIANT`	`140 140`	`1`	`I -> F (in allele Sej; non-secretor phenotype).`	`VAR_003424`
`VARIANT`	`172 172`	`1`	`D -> N (polymorphism found in Xhosa population; dbSNP:rs1800025 [NCBI]).`	`VAR_003425`
`VARIANT`	`258 258`	`1`	`G -> S.`	`VAR_022187`

Sequence information

Length: 343 AA [This is the length of the unprocessed precursor]

Molecular weight: 39017 Da [This is the MW of the unprocessed precursor]

CRC64: 12066D9CF175E13A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLVVQMPFSF PMAHFILFVF TVSTIFHVQQ RLAKIQAMWE LPVQIPVLAS TSKALGPSQL 

        70         80         90        100        110        120 
RGMWTINAIG RLGNQMGEYA TLYALAKMNG RPAFIPAQMH STLAPIFRIT LPVLHSATAS 

       130        140        150        160        170        180 
RIPWQNYHLN DWMEEEYRHI PGEYVRFTGY PCSWTFYHHL RQEILQEFTL HDHVREEAQK 

       190        200        210        220        230        240 
FLRGLQVNGS RPGTFVGVHV RRGDYVHVMP KVWKGVVADR RYLQQALDWF RARYSSLIFV 

       250        260        270        280        290        300 
VTSNGMAWCR ENIDTSHGDV VFAGDGIEGS PAKDFALLTQ CNHTIMTIGT FGIWAAYLTG 

       310        320        330        340 
GDTIYLANYT LPDSPFLKIF KPEAAFLPEW TGIAADLSPL LKH

Q10981 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools