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UniProtKB/Swiss-Prot entry Q10295


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PAP_SCHPO
Primary accession number Q10295
Secondary accession number Q9UU09
Integrated into Swiss-Prot on December 6, 2002
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 59)
Name and origin of the protein
Protein name Poly(A) polymerase pla1
Synonyms PAP
EC 2.7.7.19
Polynucleotide adenylyltransferase
Gene name
Name: pla1
ORFNames: SPBC646.04
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
DOI=10.1093/nar/24.13.2585; PubMed=8692700 [NCBI, ExPASy, EBI, Israel, Japan]
Ohnacker M., Minivielle-Sebastia L., Keller W.;
"The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue.";
Nucleic Acids Res. 24:2585-2591(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 233-436, AND SUBCELLULAR LOCATION.
STRAIN=ATCC 38364 / 968;
DOI=10.1046/j.1365-2443.2000.00317.x; PubMed=10759889 [NCBI, ExPASy, EBI, Israel, Japan]
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.;
"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library.";
Genes Cells 5:169-190(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X79705; CAA56141.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329671; CAA22808.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB027883; BAA87187.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JC6058; JC6058.
RefSeq NP_595362.1; -.
3D structure databases
HSSP P29468; 1FA0. [HSSP ENTRY / PDB]
ModBase Q10295.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-003438-MON; -.
Organism-specific databases
GeneDB_Spombe SPBC646.04; -.
Gene expression databases
ArrayExpress Q10295; -.
Ontologies
GO
GO:0005847; Cellular component: mRNA cleavage and polyadenylation specificity factor complex (inferred from genetic interaction from GeneDB_SPombe).
GO:0004652; Molecular function: polynucleotide adenylyltransferase activity (inferred from electronic annotation from InterPro).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0006378; Biological process: mRNA polyadenylation (inferred from genetic interaction from GeneDB_SPombe).
GO:0006350; Biological process: transcription (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002934; Nucleotidyltransferase.
IPR007012; PolA_pol_centr.
IPR007010; PolA_pol_RNA-bd.
IPR014492; PolyA_polymerase.
Graphical view of domain structure.
Gene3D G3DSA:3.30.70.590; PolA_pol_RNA-bd; 1.
Pfam PF01909; NTP_transf_2; 1.
PF04928; PAP_central; 1.
PF04926; PAP_RNA-bind; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF018425; PolyA_polymerase; 1.
ProtoNet Q10295.
Genome annotation databases
GeneID 2541099; -.
KEGG spo:SPBC646.04; -.
NMPDR fig|4896.1.peg.1228; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; mRNA processing; Nucleus; RNA-binding; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   566  566     Poly(A) polymerase pla1. PRO_0000051620
ACT_SITE   99    99        By similarity. 
ACT_SITE   101   101        By similarity. 
ACT_SITE   153   153        By similarity. 
Sequence information
Length: 566 AA [This is the length of the unprocessed precursor] Molecular weight: 64109 Da [This is the MW of the unprocessed precursor] CRC64: E8E0E4165AAFD3D5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTTKQWGITP PISTAPATEQ ENALNTALIN ELKNQNLFES PAESEKRVKV LDELQQITTE 

        70         80         90        100        110        120 
FVKKVSLAKH MNEKMANEAG GKIFTYGSYR LGVYGPGSDI DTLVVVPKHV SRDNFFQDLE 

       130        140        150        160        170        180 
PMLREREEVT DLAAVPDAYV PIIKFKFLGI SIDLIFARLS VPRVPRDLEL SDNNLLKGVE 

       190        200        210        220        230        240 
ERCVLSLNGT RVTDQILQLV PNRAVFKHAL RAIKFWAQRR AIYANVVGFP GGVAWAMMVA 

       250        260        270        280        290        300 
RICQLYPNAV SSVIVAKFFR ILHQWNWPQP ILLKPIEDGP LQVRIWNPKL YPSDKAHRMP 

       310        320        330        340        350        360 
IITPAYPSMC ATHNITLSTQ TIILREMVRA GEIADQIMVK ALPWSALFQK HDFFHRYKHY 

       370        380        390        400        410        420 
LTITAAAKTA EAQLKWAGLV ESKLRHLVTR LELVDAIALA HPFNKGFDKV YNCSSEEEAQ 

       430        440        450        460        470        480 
QVASGVTLEV AYESTDHEKL ANDTVNEEKA DNTESKADGS ENGEKQIFPV YTTTCYIGLE 

       490        500        510        520        530        540 
LEKKKGHPIK RLDISWPTQE FYELCKKWDK YDDTLMNVFI KNTKNTALPD EVFEPGEERP 

       550        560 
KATKKRSTAD TAHSTEQLKR QKVSTA 

Q10295 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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