FUNCTION: Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (By similarity).
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 2 BRCT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CH445341; EAT81919.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

XP_001800794.1; -.

3D structure databases

ModBase

Q0UCI9.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012309; DNA_ligase_A_C.
IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF00533; BRCT; 1.
PF04679; DNA_ligase_A_C; 1.
PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 2.
PS00697; DNA_LIGASE_A1; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q0UCI9.

Genome annotation databases

GeneID

5977701; -.

Other

ProtoNet

Q0UCI9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 990`	`990`	`DNA ligase 4.`	`PRO_0000278385`
`DOMAIN`	`728 821`	`94`	`BRCT 1.`
`DOMAIN`	`900 989`	`90`	`BRCT 2.`
`ACT_SITE`	`326 326`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 990 AA [This is the length of the unprocessed precursor]

Molecular weight: 113591 Da [This is the MW of the unprocessed precursor]

CRC64: 8B9386A5E66CAA50 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEDTEMPDA EAVRENTLMY GHDDELDEKF PTRPMNLHKS PPFHTLFTDL FDPLMETQKK 

        70         80         90        100        110        120 
GRQPPGPRRK AGPHGHSNLS PHEAKRNIIE RFIASWRKTV GNDFYPAMRL IIPDKDRDRA 

       130        140        150        160        170        180 
MYGLKEKAIA KVLIKLTKIS KDSDDAKQML NWKLPGQLHK ASASTAGDFA GRCYQVLSSR 

       190        200        210        220        230        240 
QLNTKLGDMS IAEVNNALDK LSQLGSEDEQ VKIFQRFNRR MNAEEMTWLI RIILRQMKIG 

       250        260        270        280        290        300 
ATEKTFLDIW HPDAETLFNI SSNLRRVCWE LYDPKVRLEG EDTGLSLMQC FQPQLAAFQD 

       310        320        330        340        350        360 
KGGSFEKIVA RLQANSDDDS FWIEEKLDGE RMQLHMIEDP EAPGGKLFGF WSRKAKDYAY 

       370        380        390        400        410        420 
LYGKHLQGDE AGALTRFITD AFGKNVRNII LDGEMITWDM DVDHIVGFGT LKTAAISEKE 

       430        440        450        460        470        480 
NKTDKSTGQR PLFRVFDCVY LNDKLLTPYT LRDRRRALES AVKDVKRRLE IHPYIEAHSH 

       490        500        510        520        530        540 
TEIEPALRKV VAESSEGLVL KNPRSMYRLN DRNADWMKVK PEYMSEFGES LDCIVVGGYF 

       550        560        570        580        590        600 
GSGHRGGAHS SFLCALLLNK DAKPGDADYE KCWSFFKVGG GFSREDYAAI RGRTEGKWKD 

       610        620        630        640        650        660 
WDPRRPPPII ELGGHEQNRQ HERPDQWIHP SDSVVLECKA ASVEGSDKFR FNLTLRFPRF 

       670        680        690        700        710        720 
RMLRTDKRWD QALSREEFYE IKANVEYKRE EKEKEFEIEK SRRKRTRTTK KPIVVAGSET 

       730        740        750        760        770        780 
ITTPYAGPQS KIFDGLSFYI MTEQLHPTKK SKVDLEALVK ANGGRVVQRD SMEPNLVIVA 

       790        800        810        820        830        840 
DKRLIKVASL EKRDTNNIVK PVWIQDTIQQ NEVDNGALPY LLPFEPNRHM FYLLGDNQLD 

       850        860        870        880        890        900 
YEANADDYGD SYARDIADVE EMRKILGATD PPQKRTKFDR ESFLDQLEDH GDSLSHLKTY 

       910        920        930        940        950        960 
MFSSTKVAFR TSDDPVWTLR AQLAGNYIRF GGGQITEDED EEGVTHTVVP DGQTASVSRV 

       970        980        990 
ADLSRVVGVG WVQKCWDEST RLDEERYQWG

Q0UCI9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools