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UniProtKB/Swiss-Prot entry Q08686


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name THTR_YEAST
Primary accession number Q08686
Secondary accession numbers None
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 66)
Name and origin of the protein
Protein name Putative thiosulfate sulfurtransferase
Synonym EC 2.8.1.1
Gene name
OrderedLocusNames: YOR251C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
DOI=10.1002/(SICI)1097-0061(199704)13:5<483::AID-YEA105>3.3.CO;2-L; PubMed=9153759 [NCBI, ExPASy, EBI, Israel, Japan]
Poirey R., Jauniaux J.-C.;
"Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1.";
Yeast 13:483-487(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[4]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02026; PubMed=14562095 [NCBI, ExPASy, EBI, Israel, Japan]
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 0:0-0(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z75159; CAA99473.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY692732; AAT92751.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S67148; S67148.
RefSeq NP_014894.1; -.
3D structure databases
HSSP P00586; 1RHS. [HSSP ENTRY / PDB]
ModBase Q08686.
Protein-protein interaction databases
DIP DIP:4457N; -.
IntAct Q08686; -.
Organism-specific databases
CYGD YOR251c; -.
SGD S000005777; YOR251C.
Yeast-GFP YOR251C.
Gene expression databases
ArrayExpress Q08686; -.
GermOnline YOR251C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001763; Rhodanese-like.
IPR001307; Thiosulphate_sulphurtransf_CS.
Graphical view of domain structure.
Gene3D G3DSA:3.40.250.10; Rhodanese-like; 2.
Pfam PF00581; Rhodanese; 1.
Pfam graphical view of domain structure.
SMART SM00450; RHOD; 2.
SMART graphical view of domain structure.
PROSITE PS00683; RHODANESE_2; 1.
PS50206; RHODANESE_3; 2.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q08686.
Proteomic databases
PeptideAtlas Q08686; -.
Genome annotation databases
Ensembl YOR251C; Saccharomyces cerevisiae. [Contig view]
GeneID 854425; -.
GenomeReviews Y13140_GR; YOR251C.
KEGG sce:YOR251C; -.
NMPDR fig|4932.3.peg.6006; -.
Phylogenomic databases
HOGENOM Q08686; -.
Other
LinkHub Q08686; -.
ProtoNet Q08686.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Mitochondrion; Phosphoprotein; Repeat; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   304  304     Putative thiosulfate sulfurtransferase. PRO_0000139402
DOMAIN   20   137  118     Rhodanese 1. 
DOMAIN   177   299  123     Rhodanese 2. 
ACT_SITE   259   259        Cysteine persulfide intermediate (By similarity). 
BINDING   191   191        Substrate (By similarity). 
MOD_RES   264   264        Phosphoserine. 
Sequence information
Length: 304 AA [This is the length of the unprocessed precursor] Molecular weight: 34219 Da [This is the MW of the unprocessed precursor] CRC64: F8082DF7CC279E82 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPLFDLISPK AFVKLVASEK VHRIVPVDAT WYLPSWKLDN KVDFLTKPRI PNSIFFDIDA 

        70         80         90        100        110        120 
ISDKKSPYPH MFPTKKVFDD AMSNLGVQKD DILVVYDRVG NFSSPRCAWT LGVMGHPKVY 

       130        140        150        160        170        180 
LLNNFNQYRE FKYPLDSSKV AAFSPYPKSH YESSESFQDK EIVDYEEMFQ LVKSGELAKK 

       190        200        210        220        230        240 
FNAFDARSLG RFEGTEPEPR SDIPSGHIPG TQPLPYGSLL DPETKTYPEA GEAIHATLEK 

       250        260        270        280        290        300 
ALKDFHCTLD PSKPTICSCG TGVSGVIIKT ALELAGVPNV RLYDGSWTEW VLKSGPEWIA 


ENRD 

Q08686 in FASTA format

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