[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PDE4B1 AND PDE4B2). TISSUE=Brain; PubMed=8413254 [NCBI, ExPASy, EBI, Israel, Japan] Bolger G., Michaeli T., Martins T., St John T., Steiner B., Rodgers L., Riggs M., Wigler M., Ferguson K.; "A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs."; Mol. Cell. Biol. 13:6558-6571(1993).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4B3). PubMed=9371714 [NCBI, ExPASy, EBI, Israel, Japan] Huston E., Lumb S., Russell A., Catterall C., Ross A.H., Steele M.R., Bolger G.B., Perry M.J., Owens R.J., Houslay M.D.; "Molecular cloning and transient expression in COS7 cells of a novel human PDE4B cAMP-specific phosphodiesterase, HSPDE4B3."; Biochem. J. 328:549-558(1997).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE4B2). TISSUE=Brain; PubMed=8384210 [NCBI, ExPASy, EBI, Israel, Japan] McLaughlin M.M., Cieslinski L.B., Burman M., Torphy T.J., Livi G.P.; "A low-Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain. Cloning and expression of cDNA, biochemical characterization of recombinant protein, and tissue distribution of mRNA."; J. Biol. Chem. 268:6470-6476(1993).

Comments

FUNCTION: May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H₂O = adenosine 5'-phosphate.
ENZYME REGULATION: Inhibited by rolipram.
PATHWAY: Purine metabolism; cAMP degradation; AMP from cAMP: step 1/1 .

ALTERNATIVE PRODUCTS: 3 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.

Name	PDE4B1
Isoform ID	Q07343-1
This is the isoform sequence displayed in this entry.

Name	PDE4B2
Isoform ID	Q07343-2
Features which should be applied to build the isoform sequence: VSP_004572.

Name	PDE4B3
Isoform ID	Q07343-3
Features which should be applied to build the isoform sequence: VSP_004571.

TISSUE SPECIFICITY: Expressed in brain, heart, lung and skeletal muscle.
SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L20966; AAA03589.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L20971; AAA03593.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M97515; AAA36426.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U85048; AAB96381.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I61354; I61354.

RefSeq

NP_001032416.1; -.
NP_001032418.1; -.
NP_002591.2; -.

UniGene

Hs.198072

3D structure databases

PDB

1F0J; X-ray; 1.77 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1JP1; Model; -; A=324-700.	[ExPASy / RCSB / EBI]
1JP2; Model; -; A=324-700.	[ExPASy / RCSB / EBI]
1RO6; X-ray; 2.00 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1RO9; X-ray; 2.13 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1ROR; X-ray; 2.00 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1TB5; X-ray; 2.15 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XLX; X-ray; 2.19 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XLZ; X-ray; 2.06 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XM4; X-ray; 2.31 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XM6; X-ray; 1.92 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XMU; X-ray; 2.30 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XMY; X-ray; 2.40 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XN0; X-ray; 2.31 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1XOS; X-ray; 2.28 A; A=324-700.	[ExPASy / RCSB / EBI]
1XOT; X-ray; 2.34 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1Y2H; X-ray; 2.40 A; A/B=324-700.	[ExPASy / RCSB / EBI]
1Y2J; X-ray; 2.55 A; A/B=324-700.	[ExPASy / RCSB / EBI]
2CHM; X-ray; 1.60 A; A=339-651.	[ExPASy / RCSB / EBI]
2QYL; X-ray; 1.95 A; A=324-659.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1F0J; -.
1JP1; -.
1JP2; -.
1RO6; -.
1RO9; -.
1ROR; -.
1TB5; -.
1XLX; -.
1XLZ; -.
1XM4; -.
1XM6; -.
1XMU; -.
1XMY; -.
1XN0; -.
1XOS; -.
1XOT; -.
1Y2H; -.
1Y2J; -.
2CHM; -.
2QYL; -.

ModBase

Q07343.

Protein-protein interaction databases

IntAct

Q07343; -.

PTM databases

PhosphoSite

Q07343; -.

Organism-specific databases

HIX0000677; -.

HGNC:8781; PDE4B.

HPA003005; -.

600127; gene. [NCBI / EBI]

PharmGKB

PA33129; -.

GeneCards

Q07343.

Gene expression databases

ArrayExpress

Q07343; -.

CleanEx

HS_PDE4B; -.

GermOnline

ENSG00000184588; Homo sapiens.

Ontologies

GO:0005626;	Cellular component: insoluble fraction (traceable author statement from ProtInc).
GO:0005625;	Cellular component: soluble fraction (traceable author statement from ProtInc).
GO:0004115;	Molecular function: 3',5'-cyclic-AMP phosphodiesterase activity (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR003607; Met-dep_phosphohydro_HD.
IPR002073; PDEase.
Graphical view of domain structure.

Pfam

PF00233; PDEase_I; 1.
Pfam graphical view of domain structure.

PRINTS

PR00387; PDIESTERASE1.

SMART

SM00471; HDc; 1.
SMART graphical view of domain structure.

PROSITE

PS00126; PDEASE_I; 1.

BLOCKS

Q07343.

Genome annotation databases

Ensembl

ENSG00000184588; Homo sapiens. [Contig view]

GeneID

5142; -.

KEGG

hsa:5142; -.

Phylogenomic databases

HOGENOM

Q07343; -.

HOVERGEN

Q07343; -.

Other

DrugBank

DB00131; Adenosine monophosphate.
DB01427; Amrinone.
DB00201; Caffeine.
DB01166; Cilostazol.
DB00651; Dyphylline.
DB00824; Enprofylline.
DB01113; Papaverine.
DB00806; Pentoxifylline.
DB00277; Theophylline.

PDE4B; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; cAMP; Hydrolase; Phosphoprotein; Polymorphism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 736`	`736`	`cAMP-specific 3',5'-cyclic phosphodiesterase 4B.`	`PRO_0000198809`
`MOD_RES`	`184 184`		`Phosphothreonine (By similarity).`
`MOD_RES`	`272 272`		`Phosphoserine (By similarity).`
`MOD_RES`	`274 274`		`Phosphotyrosine (By similarity).`
`VAR_SEQ`	`1 211`		`MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLW` `RGRRCCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLT` `TLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSAGLVL` `HATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQH` `GDDLIVTPFAQVLASLRSVRNNFTILTNLHGTSNKRSPAA` `SQPPVSRVNPQ -> MKKEHGGTFSSTGISGGSGDSAMDSLQPLQPNYMPVCLFA (in isoform PDE4B2).`	`VSP_004572`
`VAR_SEQ`	`1 93`		`MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLW` `RGRRCCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLT` `TLPSIAITTVSQE -> MTAKDSSKELTASEPEVCIKTFKEQMHLELELPRLPGNRP` `TSPKISPRSSPRNSPCFFRKLLVNKSIRQRRRFTVAHT (in isoform PDE4B3).`	`VSP_004571`
`VARIANT`	`703 703`	`1`	`S -> C (in dbSNP:rs2227297 [NCBI]).`	`VAR_034373`
`TURN`	`325 329`	`5`
`HELIX`	`332 341`	`10`
`HELIX`	`342 344`	`3`
`HELIX`	`352 358`	`7`
`HELIX`	`363 374`	`12`
`HELIX`	`377 380`	`4`
`HELIX`	`385 397`	`13`
`STRAND`	`403 407`	`5`
`HELIX`	`408 422`	`15`
`HELIX`	`425 427`	`3`
`TURN`	`428 430`	`3`
`HELIX`	`433 445`	`13`
`TURN`	`446 449`	`4`
`HELIX`	`455 460`	`6`
`HELIX`	`464 468`	`5`
`TURN`	`469 471`	`3`
`HELIX`	`474 485`	`12`
`HELIX`	`486 488`	`3`
`TURN`	`494 497`	`4`
`HELIX`	`500 515`	`16`
`HELIX`	`519 521`	`3`
`HELIX`	`522 534`	`13`
`HELIX`	`549 564`	`16`
`HELIX`	`567 569`	`3`
`HELIX`	`572 595`	`24`
`TURN`	`606 608`	`3`
`HELIX`	`611 621`	`11`
`HELIX`	`623 634`	`12`
`TURN`	`635 638`	`4`
`HELIX`	`639 655`	`17`
`HELIX`	`671 677`	`7`

Sequence information

Length: 736 AA [This is the length of the unprocessed precursor]

Molecular weight: 83343 Da [This is the MW of the unprocessed precursor]

CRC64: 208FCE9CD40EF5EB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKSRSVMTV MADDNVKDYF ECSLSKSYSS SSNTLGIDLW RGRRCCSGNL QLPPLSQRQS 

        70         80         90        100        110        120 
ERARTPEGDG ISRPTTLPLT TLPSIAITTV SQECFDVENG PSPGRSPLDP QASSSAGLVL 

       130        140        150        160        170        180 
HATFPGHSQR RESFLYRSDS DYDLSPKAMS RNSSLPSEQH GDDLIVTPFA QVLASLRSVR 

       190        200        210        220        230        240 
NNFTILTNLH GTSNKRSPAA SQPPVSRVNP QEESYQKLAM ETLEELDWCL DQLETIQTYR 

       250        260        270        280        290        300 
SVSEMASNKF KRMLNRELTH LSEMSRSGNQ VSEYISNTFL DKQNDVEIPS PTQKDREKKK 

       310        320        330        340        350        360 
KQQLMTQISG VKKLMHSSSL NNTSISRFGV NTENEDHLAK ELEDLNKWGL NIFNVAGYSH 

       370        380        390        400        410        420 
NRPLTCIMYA IFQERDLLKT FRISSDTFIT YMMTLEDHYH SDVAYHNSLH AADVAQSTHV 

       430        440        450        460        470        480 
LLSTPALDAV FTDLEILAAI FAAAIHDVDH PGVSNQFLIN TNSELALMYN DESVLENHHL 

       490        500        510        520        530        540 
AVGFKLLQEE HCDIFMNLTK KQRQTLRKMV IDMVLATDMS KHMSLLADLK TMVETKKVTS 

       550        560        570        580        590        600 
SGVLLLDNYT DRIQVLRNMV HCADLSNPTK SLELYRQWTD RIMEEFFQQG DKERERGMEI 

       610        620        630        640        650        660 
SPMCDKHTAS VEKSQVGFID YIVHPLWETW ADLVQPDAQD ILDTLEDNRN WYQSMIPQSP 

       670        680        690        700        710        720 
SPPLDEQNRD CQGLMEKFQF ELTLDEEDSE GPEKEGEGHS YFSSTKTLCV IDPENRDSLG 

       730 
ETDIDIATED KSPVDT

Q07343 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools