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UniProtKB/Swiss-Prot entry Q05753


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AKRP_ARATH
Primary accession number Q05753
Secondary accession numbers Q3E852 Q8GYN6 Q940Y0
Integrated into Swiss-Prot on October 1, 1994
Sequence was last modified on December 13, 2002 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 72)
Name and origin of the protein
Protein name Ankyrin repeat domain-containing protein, chloroplastic [Precursor]
Synonyms AKRP
Protein EMBRYO DEFECTIVE 2036
Gene name
Name: AKRP
Synonyms: AKR, EMB2036
OrderedLocusNames: At5g66055
ORFNames: K2A18.13
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION BY LIGHT.
STRAIN=cv. Landsberg erecta;
TISSUE=Leaf;
DOI=10.1105/tpc.4.12.1575; PubMed=1281700 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang H., Scheirer D.C., Fowle W.H., Goodman H.M.;
"Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis.";
Plant Cell 4:1575-1588(1992).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH EMB506, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
DOI=10.1111/j.1365-313X.2006.02922.x; PubMed=17092312 [NCBI, ExPASy, EBI, Israel, Japan]
Garcion C., Guilleminot J., Kroj T., Parcy F., Giraudat J., Devic M.;
"AKRP and EMB506 are two ankyrin repeat proteins essential for plastid differentiation and plant development in Arabidopsis.";
Plant J. 48:895-906(2006).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/5.2.131; PubMed=9679202 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones.";
DNA Res. 5:131-145(1998).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[6]
TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
STRAIN=cv. C24;
PubMed=12232406 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang H., Wang J., Goodman H.M.;
"Expression of the Arabidopsis gene Akr coincides with chloroplast development.";
Plant Physiol. 106:1261-1267(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M82883; AAA32812.1; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB011474; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AK117484; BAC42148.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY052363; AAK96554.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT001055; AAN46809.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00523216; -.
IPI00545683; -.
PIR JQ1729; JQ1729.
RefSeq NP_569027.2; -.
NP_975000.1; -.
UniGene At.68506
3D structure databases
HSSP P80144; 2MYO. [HSSP ENTRY / PDB]
ModBase Q05753.
Protein-protein interaction databases
IntAct Q05753; 1.
Organism-specific databases
GeneFarm 2586; 230.
TAIR At5g66055; -.
Gene expression databases
ArrayExpress Q05753; -.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from direct assay from TAIR).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR002110; ANK.
Graphical view of domain structure.
Gene3D G3DSA:1.25.40.20; ANK; 1.
Pfam PF00023; Ank; 5.
Pfam graphical view of domain structure.
SMART SM00248; ANK; 5.
SMART graphical view of domain structure.
PROSITE PS50297; ANK_REP_REGION; 1.
PS50088; ANK_REPEAT; 2.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 836737; -.
GenomeReviews BA000015_GR; AT5G66055.
KEGG ath:AT5G66055; -.
NMPDR fig|3702.1.peg.28700; -.
Phylogenomic databases
OMA Q05753; LLRNSAN.
Other
ProtoNet Q05753.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; ANK repeat; Chloroplast; Complete proteome; Plastid; Repeat; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    36  36     Chloroplast (Potential). 
CHAIN   37   435  399     Ankyrin repeat domain-containing protein, chloroplastic. PRO_0000001621
REPEAT   259   288  30     ANK 1. 
REPEAT   292   321  30     ANK 2. 
REPEAT   325   354  30     ANK 3. 
REPEAT   358   387  30     ANK 4. 
REPEAT   391   424  34     ANK 5. 
VAR_SEQ   358   358        D -> V (in isoform 2). VSP_030158
VAR_SEQ   360   435        Missing (in isoform 2). VSP_030159
CONFLICT   16    16        R -> G (in Ref. 1; AAA32812). 
CONFLICT   30    30        A -> V (in Ref. 1; AAA32812). 
CONFLICT   73    73        D -> E (in Ref. 1; AAA32812). 
CONFLICT   105   105        L -> P (in Ref. 4; BAC42148). 
CONFLICT   146   146        P -> Q (in Ref. 4; BAC42148). 
CONFLICT   181   181        S -> F (in Ref. 1; AAA32812). 
CONFLICT   296   296        V -> A (in Ref. 1; AAA32812). 
CONFLICT   406   406        I -> T (in Ref. 1; AAA32812). 
Sequence information
Length: 435 AA [This is the length of the unprocessed precursor] Molecular weight: 48878 Da [This is the MW of the unprocessed precursor] CRC64: DE306D487CC894EC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MQSLSTPHTI SLLLPRTSPS RLSPSLHSLA FPTRLRSLSY SSQTSILPDA GDDFIVGDCL 

        70         80         90        100        110        120 
VYEDGVFEDP YLDKEVTQVA KQERKKNRRG GAKRLDESEI EPENLVPEEW RDIQAEVNLT 

       130        140        150        160        170        180 
KKDKRKIAQE MEFGVRVEKK RQGLIPLRKV DLNDFLTYKE AKLAQLRPVI LDKPGNFSDD 

       190        200        210        220        230        240 
SGASSDGETA VSSPSERVAP KNPRWAVYGK GFDHVAKFFN SDKYDPSDKK SDGPRKLLSK 

       250        260        270        280        290        300 
EEKFMLNSRN PDLAVATSKK WLPLHTLAAC GEFYLVDSLL KHNLDINATD VGGLTVLHRA 

       310        320        330        340        350        360 
IIGKKQAITN YLLRESANPF VLDDEGATLM HYAVQTASAP TIKLLLLYNA DINAQDRDGW 

       370        380        390        400        410        420 
TPLHVAVQAR RSDIVKLLLI KGADIEVKNK DGLTPLGLCL YLGREIRTYE VMKLLKEFPL 

       430 
SRHKKRLVTT DEDIE 

Q05753 in FASTA format

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